The use of light-emitting diodes (LEDs) for plant production is a new field of research that has great promise to optimize wavelength-specific lighting systems for precise management of plant physiological responses and important secondary metabolite production. In our experiment, hydroponically cultured kale plants (Brassica oleracea L. var. acephala D.C.) were grown under specific LED wavelength treatments of 730, 640, 525, 440, and 400 nm to determine changes in the accumulation of chlorophylls, carotenoids, and glucosinolates. Maximum accumulation, on a fresh mass basis, of chlorophyll a and b and lutein occurred at the wavelength of 640 nm, whereas β-carotene accumulation peaked under the 440-nm treatment. However, when lutein was measured on a dry mass basis, maximum accumulation was shifted to 440 nm. Sinigrin was the only glucosinolate to respond to wavelength treatments. Wavelength control using LED technology can affect the production of secondary metabolites such as carotenoids and glucosinolates with irradiance levels also a factor in kale. Management of irradiance and wavelength may hold promise to maximize nutritional potential of vegetable crops grown in controlled environments.
Mark G. Lefsrud, Dean A. Kopsell and Carl E. Sams
Dean A. Kopsell, Carl E. Sams and Robert C. Morrow
Light is one of the most important environmental stimuli impacting plant growth and development. Plants have evolved specialized pigment-protein complexes, commonly referred to as photoreceptors, to capture light energy to drive photosynthetic processes, as well as to respond to changes in light quality and quantity. Blue light can act as a powerful environmental signal regulating phototropisms, suppression of stem elongation, chloroplast movements, stomatal regulation, and cell membrane transport activity. An emerging application of light-emitting diode (LED) technology is for horticultural plant production in controlled environments. Work by our research group is measuring important plant responses to different wavelengths of light from LEDs. We have demonstrated positive impacts of blue wavelengths on primary and secondary metabolism in microgreen and baby leafy green brassica crops. Results show significant increases in shoot tissue pigments, glucosinolates, and essential mineral elements following exposure to higher percentages of blue wavelengths from LED lighting. The perception of energy-rich blue light by specialized plant photoreceptors appears to trigger a cascade of metabolic responses, which is supported by current research showing stimulation of primary and secondary metabolite biosynthesis following exposure to blue wavelengths. Management of the light environment may be a viable means to improve concentrations of nutritionally important primary and secondary metabolites in specialty vegetable crops.
John M. Kauffman, John C. Sorochan and Dean A. Kopsell
Thatch-mat and organic matter (OM) accumulation near the putting green soil surface impacts soil physical properties and turf performance. Excessive thatch and OM can encumber infiltration of water and oxygen into the soil profile and slow drainage of excess water away from the putting surface. Proper sampling of thatch-mat depths and OM contents is vital for management of putting green turf; therefore, a study was performed in Knoxville, TN, to derive proper sampling procedures of these important parameters using ‘TifEagle’ and ‘Champion’ bermudagrass (Cynodon dactylon × C. transvaalensis), ‘SeaDwarf’ seashore paspalum (Paspalum vaginatum), and ‘Diamond’ zoysiagrass (Zoysia matrella). ‘TifEagle’ and ‘Champion’ accumulated thatch-mat to a greater depth than ‘SeaDwarf’ and ‘Diamond’. However, ‘SeaDwarf’ had a higher OM content than ‘Diamond’ and both had higher OM contents than ‘TifEagle’ and ‘Champion’. Data generated from sampling procedures indicate that previous studies often undersampled plots for thatch-mat depth; however, previous sampling procedures have not traditionally undersampled plots for OM. Data in this study provide a range of confidence and minimum detectable difference levels which may allow future researchers to more accurately sample ‘TifEagle’, ‘Champion’, ‘SeaDwarf’, and ‘Diamond’ putting green plots for thatch-mat depth and OM content.
Vincent Martineau, Mark Lefsrud, Most Tahera Naznin and Dean A. Kopsell
Recent irradiance level improvements in light-emitting diode (LED) technology has allowed this equipment to compete as suitable replacements to traditional high-pressure sodium (HPS) lamps in hydroponics growth environments. The current study compares LED and HPS lighting technologies for supplemental lighting in a greenhouse at HydroSerre Mirabel (Mirabel, Quebec, Canada) for the growth of Boston lettuce (Lactuca sativa var. capitata). The light treatments were applied for 2 hours before sunset and 8.5 hours after sunset to extend the photoperiod to 18 hours. An average total light irradiance (natural and supplemental) of 71.3 mol·m−2 for HPS and 35.8 mol·m−2 for LED were recorded over the 4 weeks of each experimental run. Wet and dry biomass of the shoots was recorded. On average, HPS light treatments produced significantly similar shoot biomass compared with LED light treatment, although the LED lamps provided roughly half the amount of supplemental light compared with the HPS lamps during the 4 weeks of the experimental treatment. Analysis of the lettuce samples showed no significant difference in concentrations of β-carotene, chlorophyll a, chlorophyll b, neoxanthin, lutein, and antheraxanthin among the light treatments; however, violaxanthin concentrations showed a statistical difference resulting from light treatment. When measured on an energy basis, the LED lamps provide an energy savings of at least 33.8% and the minimal “regular” HPS provided an energy savings of 77.8% over the HPS treatment.
Dean A. Kopsell, David E. Kopsell, Mark G. Lefsrud, Joanne Curran-Celentano and Laura E. Dukach
Green leafy vegetables are important sources of dietary carotenoids, and members of Brassica oleracea L. var. acephala rank highest for reported levels of lutein and β-carotene. Twenty-three leafy B. oleracea cultigens were field grown under similar fertility over two separate years and evaluated for leaf lutein and β-carotene accumulation. Choice of B. oleracea cultigen and year significantly affected carotenoid levels. Lutein concentrations ranged from a high of 13.43 mg per 100 g fresh weight (FW) for B. oleracea var. acephala `Toscano' to a low of 4.84 mg/100 g FW for B. oleracea var. acephala 343-93G1. β-carotene accumulations ranged from a high of 10.00 mg/100 g FW for B. oleracea var. acephala `Toscano' to a low of 3.82 mg/100 g FW for B. oleracea var. acephala 30343-93G1. Carotenoid concentrations were significantly higher in year 2 than in year 1, but rank order among the cultigens for both lutein and ß-carotene did not change between the years. During each year, there were high correlations between leaf carotenoid and chlorophyll pigments. Under similar growing conditions, choice of B. oleracea cultigen will influence carotenoid accumulation, and this may affect the health benefits of consuming these leafy green vegetable crops.
Dean A. Kopsell, J. Scott McElroy, Carl E. Sams and David E. Kopsell
Vegetable crops can be significant sources of nutritionally important dietary carotenoids, and Brassica are sources that also exhibit antioxidant and anticarcinogenic activity. The family Brassicaceae contains a diverse group of plant species commercially important in many parts of the world. The six economically important Brassica species are closely related genetically. Three diploid species (B. nigra, B. rapa, B. oleracea) are the natural progenitors of the amphidiploid species (B. juncea, B. napus, B. carinata). The objective of this study was to characterize the accumulation of important dietary carotenoid pigments among the genetically related Brassica species. High-performance liquid chromatographic quantification revealed significant differences in carotenoid and chlorophyll pigment concentrations among the Brassica species. Brassica rapa accumulated the highest concentrations of antheraxanthin [0.79 mg/100 g fresh weight (FW)], lutein (8.89 mg/100 g FW), and zeaxanthin (0.75 mg/100 g FW). The highest concentrations of β-carotene (4.41 mg/100 g FW) and total chlorophyll (125.9 mg/100 g FW) were found in B. juncea. Brassica nigra accumulated the highest concentrations of 5,6-epoxylutein (0.41 mg/100 g FW) and violaxanthin (2.28 mg/100 g FW), whereas B. oleracea accumulated the highest concentrations of neoxanthin (2.10 mg/100 g FW). For many of the pigments analyzed, the amphidiploids B. carinata and B. napus accumulated significantly less carotenoid concentrations than the diploid species and B. juneca. Brassica convey unique health attributes when consumed in the diet. Identification of genetic relationships among the Brassica species would be beneficial information for improvement programs designed to increase carotenoid values.
Mark G. Lefsrud, Dean A. Kopsell, David E. Kopsell and Joanne Curran-Celentano
Crop plants are adversely affected by a variety of environmental factors, with air temperature being one of the most influential. Plants have developed a number of methods in the adaptation to air temperature variations. However, there is limited research to determine what impact air temperature has on the production of secondary plant compounds, such as carotenoid pigments. Kale (Brassica oleracea L.) and spinach (Spinacia oleracea L.) have high concentrations of lutein and β-carotene carotenoids. The objectives of this study were to determine the effects of different growing air temperatures on plant biomass production and the accumulation of elemental nutrients, lutein, β-carotene, and chlorophyll pigments in the leaves of kale and spinach. Plants were grown in nutrient solutions in growth chambers at air temperatures of 15, 20, 25, and 30 °C for `Winterbor' kale and 10, 15, 20, and 25 °C for `Melody' spinach. Maximum tissue lutein and β-carotene concentration occurred at 30 °C for kale and 10 °C for spinach. Highest carotenoid accumulations were 16.1 and 11.2 mg/100 g fresh mass for lutein and 13.0 and 10.9 mg/100 g fresh mass for β-carotene for the kale and spinach, respectively. Lutein and β-carotene concentration increased linearly with increasing air temperatures for kale, but the same pigments showed a linear decrease in concentration for increasing air temperatures for spinach. Quantifying the effects of air temperature on carotenoid accumulation in kale and spinach, expressed on a fresh mass basis, is important for growers producing these crops for fresh markets.
Dean A. Kopsell, Carl E. Sams, T. Casey Barickman, Dennis E. Deyton and David E. Kopsell
Selenium (Se) is an essential mammalian micronutrient. Adult humans have a daily requirement of 55 to 70 μg/day Se depending on sex and pregnancy/lactation for females. In addition, recent studies have shown health benefits with dietary Se supplementation of 100 to 200 μg/day Se. However, daily intakes in humans greater than 900 μg Se will result in toxicity called selenosis. Although not essential in plant nutrition, some species can bioaccumulate Se. Brassica and Allium species became prime candidates for Se enrichment because of their ability to accumulate and tolerate high concentrations of Se in edible tissues; however, there is now concern that these species are too efficient at selenization and overconsumption of their selenized tissues could result in selenosis. Herbal crop species are consumed regularly in the diet for their culinary flavor attributes. Basil (Ocimum basilicum L.) and cilantro (Coridandrum sativum L.) are not classified as Se accumulators. Therefore, a study was undertaken to determine the potential to selenize basil and cilantro through foliar Se applications to consistently supplement diets with nutritionally beneficial levels of Se. Plants of each species were grown in both growth chamber and field environments and treated with foliar applications (5 mL per plant) of selenate-Se and selenite-Se at concentrations of 0, 2, 4, 8, 16, and 32 mg·L−1 Se. Crops received three separate foliar applications at ≈5-day intervals beginning 24 to 28 days after planting for the growth chamber plants and 50 days after planning for the field environment. Selenium accumulation in both basil and cilantro leaf tissues increased linearly under both selenate-Se (P ≤ 0.001) and selenite-Se (P ≤ 0.001) foliar treatments in growth chamber and field evaluations. Maximum Se leaf tissue concentrations for basil and cilantro ranged from 13 to 55 μg·g−1 Se dry weight. Selenization of basil and cilantro is possible through foliar Se applications, and Se fortification of herbal crops may provide alternative delivery systems in human diets.
Dean A. Kopsell, Kimberly J. Whitlock, Carl E. Sams and David E. Kopsell
Purslane (Portulaca oleracea) is a succulent weedy annual in much of the United States. In other parts of the world, purslane is grown as a specialty crop, valued for its nutritional quality. As a leafy crop, purslane contributes carotenoid phytochemicals in the typical Mediterranean diet. Nitrogen (N) influences plant growth and alters pigment composition and accumulation. However, little is known about the impact N fertility may have on pigment concentrations in purslane shoot tissues. The objective of this study was to evaluate the influence of N fertility levels on biomass and concentrations of nutritionally important carotenoid and chlorophyll pigments in purslane. Green Leaf and Golden Leaf purslane cultivars were grown in nutrient solution culture under N concentrations of 13, 26, 52, or 105 mg·L−1. Plants were harvested at 45 days after planting (DAP), and measured for concentrations of shoot pigments using high-performance liquid chromatography (HPLC) methodology. There was no influence of N treatment concentration on purslane shoot tissue fresh weight (FW) accumulation. Nitrogen treatment significantly influenced shoot tissue β-carotene (BC), lutein (LUT), neoxanthin (NEO), total carotenoids, chlorophyll a, chlorophyll b, total chlorophyll, and the chlorophyll a to b ratio in purslane shoot tissues. Concentrations of LUT, NEO, violaxanthin (VIO), chlorophyll b, total xanthophyll cycle pigments, and the chlorophyll a to b ratio differed between the purslane cultivars. Increases in N concentrations acted to increase concentrations of nutritionally important shoot tissue carotenoid pigments in only the Green Leaf purslane cultivar. Therefore, N fertility management and cultivar selection should be considered when producing purslane as a nutritious specialty vegetable crop.
Mark G. Lefsrud, Dean A. Kopsell, Robert M. Augé and A.J. Both
Consumption of fruit and vegetable crops rich in lutein and β-carotene carotenoids is associated with reduced risk of cancers and aging eye diseases. Kale (Brassica oleracea L. var. acephala D.C.) ranks highest for lutein concentrations and is an excellent source of dietary carotenoids. Kale plants were grown under varied photoperiods to determine changes in the accumulation of fresh and dry biomass, chlorophyll a and b, and lutein and β-carotene carotenoids. The plants were cultured in a controlled environment using nutrient solutions under photoperiod treatments of 6, 12, 16, or 24 hours (continuous). Fresh and dry mass production increased linearly as photoperiod increased, reaching a maximum under the 24-hour photoperiod. Maximum accumulation of lutein, β-carotene, and chlorophyll b occurred under the 24-h photoperiod at 13.5, 10.4, and 58.6 mg/100 g fresh mass, respectively. However, maximum chlorophyll a (235.1 mg/100 g fresh mass) occurred under the 12-hour photoperiod. When β-carotene and lutein were measured on a dry mass basis, the maximum accumulation was shifted to the 16-hour photoperiod. An increase in photoperiod resulted in increased pigment accumulation, but maximum concentrations of pigments were not correlated with maximum biomass production.