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Abstract
Seven enzyme systems were examined in 69 apricot [Prunus armeniaca L. and P. mandshurica (Maxim.) Koehne] clones. Three enzymes (6-phosphogluconate dehydrogenase, phosphoglucose isomerase, and phosphoglucomutase) were polymorphic at five loci. Only seven clones were characterized uniquely by their isozyme phenotypes and 56% fell into two of the 15 phenotypic groups found. Isozyme variability in apricot was greater than in peach, but less than that reported in plum or almond.
Abstract
Twenty-nine Japanese-type plum clones were assayed for isozymic variability for eight enzyme systems. Glutamate dehydrogenase (GDH), leucine amino-peptidase (LAP), malate dehydrogenase (MDH), phosphoglucose isomerase (PGI), phosphoglucomutase (PGM), and peroxidase (PX) showed variability among the plums surveyed. 6-phosphogluconate dehydrogenase (6PGD) and triosephosphate isomerase (TPI) were not variable. Isozymic characterization uniquely identified 38% of the clones. The remainder separated into groups of two to three clones that were distinguishable using vegetative morphological characteristics. Reported parentage of five out of nine plums examined was not consistent with their isozymic genotypes.
Mild winter weather conditions reduce fruit yield and quality of many peach cultivars grown in the Medium Chill Region of the United States. Peach fruit shape instability limits marketing options for growers in this region. The Stonefruit Breeding Program at Texas A&M University evaluated a wide range of peach cultivars and breeder selections from throughout the world during the mild winters of 1988-198 9 and 1989-1990. Fruit shape response was highly variable among genotypes with similar chilling requirements. The implication of this is that the potential is high for eliminating fruit shape instability due to highly variable winter conditions in the Medium Chill Peach Production Region.
`TexPrince' is being released by Texas A&M University to provide a medium chilling, commercially acceptable peach that ripens after `Texstar' and with or a few days after `Juneprince' and `TexRoyal'. This large, freestone, attractive, yellow-flesh peach ripens early June in the medium chill zones of the United States. `TexPrince' is a replacement for `Juneprince' where `Juneprince' fruit inconsistently due to problems with insufficient chilling.
Carbohydrate source of peach [Prunus persica (L.) Batsch] embryo culture media affects embryo growth and survival. The first objective of this study was to determine the effect of five carbohydrates (fructose, glucose, maltose, sorbitol, and sucrose) in Woody Plant Medium (WPM) on the germination and survival of peach embryos in vitro. Fructose, glucose, maltose, and sucrose in WPM resulted in better embryo germination and survival than sorbitol. Fructose (2% and 3%) produced greater survival than all other carbohydrates tested in smaller embryos (<10% ovule dry weight). However, sucrose was better than all other carbohydrates tested in the larger embryos (≥10% ovule dry weight). In addition, large embryos (>10% ovule dry weight) on fructose at 1% combined with glucose, maltose, sorbitol, or sucrose at 1% had equivalent or higher survival than did those on either 1% or 2% sucrose in conjunction with the same carbohydrates. Embryo survival on different carbohydrates varied with genotype. The second objective of this study was to determine the effect of three levels of MES buffer (0.0 mm, 4.5 mm, and 9.0 mm) on medium pH stability and embryo survival. MES buffer at 0.0 mm and 4.5 mm concentration produced significantly better embryo survival than 9.0 mm. The pH stability was better at MES 9.0 mm, however survival decreased significantly. Chemical name used: [2-(N-morpholino)-ethane sulphonic acid] (MES)
Isozyme analysis has been used for cultivar identification, but little has been done with the genus Rosa. One hundred and sixty rose accessions (species, cultivars, and hybrids) were characterized for isozyme phenotypes using starch gel electrophoresis. Six enzyme systems were stained on three electrode buffer systems. ACP, MDH, and 6PGD were run on morpholine citrate (pH 6.1) and histidine (pH 5.7), PGI and PGM were run on histidine (pH 5.7) and lithium borate (pH 8.3), and SKDH was run on morpholine citrate (PH 6.1) and lithium borate (PH 8.3). The most variable isozymes were MDH and 6PGD. MDH and 6PGD revealed 10 and 9 bands, respectively. This study showed that isozyme variability exists in roses and can be useful in their classification into species groups.
Six controlled crosses of cultivated and advanced selection Japanese-type plums adapted to southeast and southwest regions of the United States were made in 1990 and 1991. Over 800 seedlings from these crosses along with open pollinated seedlings of the parents were established in Suiting nurseries. The long range objective of this study is to determine linkage relationships between RAPD markers and commercially important traits (soluble solid, resistance to bacterial leaf spot, chilling requirement, fruit development period). The first step in the projects to characterize RAPD genotypes in the progenies. Eighty oligodecamers have been screened and 57 yielded successful reactions with an average of two to three bands per primer. The variability and inheritance of the RAPD markers in these plum populations will be described.