Search Results

You are looking at 11 - 15 of 15 items for

  • Author or Editor: Yan Ma x
Clear All Modify Search

The pathogenic fungus Colletotrichum musae infects developing green bananas (Musa spp. AAA group), but remains latent until the fruit ripens. The aim of this research was to determine whether the appearance of disease symptoms is regulated by chitinase gene expression following treatment of fruit with benzothiadiazole (BTH) and methyl jasmonate (MeJA), and with physical (heat) and chemical (H2O2 and Ca2+-related) treatments. In bananas inoculated with C. musae, BTH and MeJA lowered disease severity and stimulated higher gene expression compared with the untreated controls during ripening. However, in naturally infected bananas, BTH and MeJA treatments slightly reduced transcription of the chitinase gene in green bananas, but they prolonged gene expression in ripe bananas and significantly reduced disease severity. The combination of H2O2 and the NADPH oxidase inhibitor, diphenylene iodonium, down-regulated chitinase gene expression and compromised disease resistance compared with H2O2 alone. Heat treatment (HT) or the combination of HT followed by CaCl2 reduced disease, but only the latter significantly upregulated chitinase gene expression. The combination of HT and a calcium ionophore (A23187) resulted in different disease indicies and different levels of gene expression depending upon the order of application: HT followed by A23187 induced higher gene expression and lower disease. The results suggest that disease resistance of green bananas could be related to high and prolonged levels of chitinase gene expression, and chitinase could be involved in harvested banana's anthracnose resistance activated by different defense pathway signals, such as BTH, MeJA, H2O2, and Ca2+.

Free access

Acer truncatum seeds are an excellent source of beneficial natural compounds, including high levels of unsaturated fatty acids (UFAs), that promote health. Recently, A. truncatum has emerged as an oil crop. Therefore, the transcriptomes of A. truncatum seeds at 70, 85, 100, 115, 145, 180 days after flowering (DAF) were analyzed to gain a better understanding of the transcriptional and translational regulation of seed development and oil biosynthesis. A total of 28,438 genes were identified, and 3069/2636, 3288/3438, 1319/2750, and 5724/5815 upregulated/downregulated genes were identified when comparing different samples with 85 DAF seeds. Sixteen lipid metabolism pathways with 754 differentially expressed genes (DEGs) were identified, including 34 DEGs associated with UFA biosynthesis. A phylogenetic analysis revealed that six putative fatty acid desaturase (FAD) genes clustered into five FAD groups. A quantitative real-time polymerase chain reaction analysis indicated that the temporal expression patterns of oil biosynthesis genes and transcription factors were largely similar to the RNA sequencing results. The results of this study will enhance the current understanding of oil metabolism in A. truncatum seeds and allow new methods of improving oil quality and seed yield in the future.

Open Access

This study aimed to clarify the relationship between grape (Vitis vinifera L.) quality and environmental factors (climate and soil), and to elucidate the theoretical basis and provide technical guidance for the rational planning of the cultivation area and the precise regulation of the cultivation mode in the future. The fruits of three different grape cultivars, Pinot Noir, Merlot, and Vidal, as well as soil samples and meteorological data were collected from three wine grape growing areas (Wuwei, Zhangye, and Jiayuguan) in the Hexi Corridor, Northwest China. Principal-component analysis (PCA) and correlation analysis were conducted to understand the relationship of grape quality with weather conditions and soil properties. The results showed that the titratable acid content of grape berries was significantly negatively correlated with average temperature from August to September, average minimum temperatures from August to September, and from April to October, mean annual minimum temperature, growing degree days from August to September, and soil total nitrogen content, and significantly positively correlated with average rainfall from April to October, annual mean rainfall, and soil available potassium content. In addition, the pH of grape juice was significantly negatively correlated with annual mean rainfall and soil available potassium content. However, it was significantly positively correlated with average temperature and average minimum temperature from August to September, average minimum temperature from April to October, growing degree days from August to September, as well as soil total nitrogen content. In addition, the results of PCA showed that the fruit quality scores of ‘Vidal’ in Jiayuguan, ‘Merlot’ in Zhangye, and ‘Pinot Noir’ in Wuwei were the highest, respectively. In conclusion, the contents of titratable acid, pH, and Brix° are greatly affected by climate and soil factors. ‘Vidal’, ‘Merlot’, and ‘Pinot Noir’ were suitable for planting in Jiayuguan, Zhangye, and Wuwei, respectively. Nowadays, few reports focus on the relationship between grape quality and soil and climate conditions. Based on the investigation and analysis of the differences of climate, soil, and grape berries quality in the three production areas of Hexi Corridor, the relationship among climate, soil, and grape quality by using statistical methods was studied, which could provide references for clarifying the reasons why environmental factors affect grape quality and select the suitable area for wine grape cultivation.

Open Access

To our knowledge, there has been no published technique to produce consistently high-quality slides of somatic chromosomes of roses (Rosa sp.). Therefore, various pretreatments, fixatives, digestions, stains, and maceration and squashing methods were tested to identify a procedure to produce clear, well-spread chromosomes from shoot tips. The best results were obtained after pretreatment in a mixture of 0.1% colchicine and 0.001 m 8-hydroxyquinoline for 4 h, and fixation in 2 acetone: 1 acetic acid (v/v) with 2% (w/v) polyvinylpyrrolidone. The darkest-stained chromosomes were obtained with carbol-fuchsin staining of air-dried cell suspensions that had been spread in 3 ethanol: 1 acetic acid (v/v).

Free access