Tissue nitrate (NO3) concentration (TNC) in leafy greens generally decreases with increasing light intensity and photoperiod in controlled environment studies. Harvesting late in the day has been recommended as a way to produce leafy greens with lower TNC, although data from field research do not support this recommendation. This study investigated the effect of time of day of harvest on TNC in lettuce (Lactuca sativa L.) and spinach (Spinacia oleracea L.) grown in the field during the summer at Pullman, WA (lat. 46° N) and Fairbanks, AK (lat. 64° N). Whole plants were sampled every 2 h on three separate, 24-h harvest dates at each latitude. Plants were dried, ground, and analyzed for NO3-N. At the high-latitude location, TNC decreased linearly during the day (1000 to 2300 hr) on all three dates for spinach and one for lettuce. At the low-latitude location, TNC decreased linearly during the day (1000 to 1900 hr) on one date and increased linearly during the night (2000 to 0400 hr) on two dates for lettuce. The TNC (average 287 to 607 mg NO3-N/kg fresh weight for lettuce and 141 to 189 mg NO3-N/kg fresh weight for spinach) and magnitude of diurnal fluctuation (generally less than 25%) should not pose a human health risk regardless of when plants are harvested.