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Greenhouse rose (Rosa × spp. L.) production is facing the use of poor-quality irrigation waters and regulatory pressures to recycle runoff and drainage effluents. Two experiments (were conducted to evaluate the yield and quality and ion accumulation responses of roses grafted on various rootstocks to increasing salinity stress. In Expt. 1, the scion ‘Bridal White’ grafted on ‘Manetti’, R. odorata (Andr.), ‘Natal Briar’, and ‘Dr. Huey’ were irrigated over four flowering cycles with complete nutrient solutions supplemented with NaCl at 0, 5, and 30 mm. In Expt. 2, plants of ‘Red France’ on ‘Manetti’ and ‘Natal Briar’ were irrigated over six flowering cycles with complete nutrient solutions supplemented with NaCl + CaCl₂ (2:1 m ratio) at 0, 1.5, 3, 6, 12, and 24 mm. Salt concentration increases significantly and negatively affected the biomass, cut flower production, and foliage quality of the roses in both experiments, but the responses were modulated by rootstock selection. ‘Manetti’ plants in general sustained better absolute and relative biomass and flower yields, accumulated less Na⁺ and Cl⁻ in its tissues, and showed less toxicity symptoms with increasing salinity than the others. ‘Natal Briar’ also had similar absolute productivity responses as ‘Manetti’ but were afflicted by a significantly different mineral nutrient profile, including higher accumulations and toxicities with Na⁺ and Cl⁻ that led to lower foliage visual ratings. Conversely, the relative yields of plants on ‘Dr. Huey’ and R. odorata were similarly reduced by increasing salinity, but the former had lower Na⁺ and Cl⁻ concentrations in its tissues and better visual scores than the latter, which fared as the worst. A combined analysis of the results suggests that on a productivity basis (biomass and flower yields), greenhouse roses could withstand overall maximum electrical conductivities (i.e., osmotic effects) of applied fertigation solutions of 3.0 ± 0.5 dS·m⁻¹. On the other hand, and considering the aesthetic responses (visual scores) of on-plant and harvested foliage (cut flower shoots), greenhouse rose tolerance to applied Na⁺ and Cl⁻ concentrations (ion-specific effects) could range up to 10 ± 2 mm.

Abstract

There was an accumulation of total free amino acids (calculated as the sum of the individual amino acids) in corollas from cut ‘Sonia’ roses (Rosa hybrida) stored at 2C (cold-stored flowers) but not in those kept at 20C (control flowers). In cold-stored flowers, senescence was retarded, as indicated by only a slight opening of the corolla and no subsequent petal abscission. Hence, there appeared to be no direct correlation between senescence of cut roses and accumulation of total amino acids in corollas; neither was there a relationship between individual or total free amino acids and protease activity in the corollas from either cold-stored or control flowers. Changes in the contents of all free amino acids, except alanine and lysine, were affected by cold storage. The effect on aspartic acid was statistically significant, but not spectacular. Cold storage delayed the decrease in contents of glutamic acid, asparagine, tyrosine, glycine, leucine, isoleucine and valine, and prevented accumulation of phenylalanine, proline, and histidine. We detected only one theoretically expected interconversion between two amino acids; i.e., glutamic acid to proline, that occurred in corollas from control flowers during the first 6 days of storage. We suggest that the patterns of changes in the contents of tyrosine, valine, isoleucine (or isoleucine plus leucine), and phenylalanine are not restricted to the cultivar Sonia.

Antioxidants, antioxidant capacity, and the expression of isoprenoid metabolism–related genes and two pigmentation-related transcription factors were studied in four native and four hybrid tomato (Solanum lycopersicum) genotypes with different-colored fruit. Red fruit genotypes were associated with greater lycopene, β-carotene, lipophilic antioxidant capacity, and greater chromoplast-specific lycopene β-cyclase (CYC-B) transcript levels. Orange fruit genotypes had greater concentrations of tocopherols and greater transcript levels of homogentisate phytyl transferase (VTE-2), 1-deoxy-D-xylulose phosphate synthase (DXS), and 4-hydroxyphenylpyruvate dioxygenase (HPPD). The yellow fruit genotype was greater in total polyphenol and hydrophilic antioxidant capacity with greater expression of geranylgeranyl reductase (GGDR), phytol kinase (VTE-5), phytoene synthase (PSY) 2, lycopene β-cyclase (LCY-B), SlNAC1, and SINAC4. Greater levels of individual antioxidants were associated with specific coloration of tomato fruit. Moreover, the negative correlations between the expression of PSY1 and VTE-5, and between lycopene and chlorophyll, suggest a balance between carotenoids, tocopherols, and chlorophylls. The results of this study support either the direct commercialization of tomatoes with different color fruit or use of their genotypes in breeding programs to increase antioxidant levels among existing cultivars.

The differences in growth and yield in the Junebearing strawberry (Fragaria ×ananassa Duch.) `Elsanta' and the everbearing `Bolero' and `Everest' were evaluated under field conditions. The seasonal patterns of radiation use efficiency and assimilate partitioning were also studied. Growth, development and yield showed considerable differences among cultivars. `Elsanta' showed the highest and `Bolero' and `Everest' the lowest values for almost all the vegetative parameters (leaf area, leaf dry weight, runner number). `Elsanta' produced large leaves and few crowns per plant in contrast to the everbearing cultivars which had more but smaller leaves and a larger number of crowns per plant. The production of flowers by `Elsanta' was concentrated in June with fruit production following in July. `Bolero' and `Everest' produced more than one flush of flowers during the season and fruited until October. As a result, yields of `Bolero' and `Everest' were greater than `Elsanta'. The higher yields of `Bolero' and `Everest' also reflected the greater number of crowns produced by these cultivars. The maximum intercepted and absorbed photosynthetically active radiation (PAR) occurred between July and August when the three cultivars showed the greatest increase in vegetative growth. Harvest index clearly differed among cultivars and this was related to the duration of cropping. The greatest harvest indexes were found for `Bolero' and `Everest'.

Mulch (black plastic, wheat straw, or bare ground) and irrigation (drip or overhead sprinkler) treatments were evaluated for their effect on center rot of onion (Allium cepa L.), caused by the bacterium Pantoea ananatis, over the course of two seasons. Irrigation type had no effect on center rot incidence or severity in either year. In contrast, center rot development was delayed by 7 to 14 days on onions grown in straw mulch or bare ground compared to those in black plastic. Straw mulch resulted in later harvest dates and was associated with reduced levels of center rot. In contrast, black plastic increased disease incidence and hastened the onset of the epidemic. The spatial distribution of disease incidence in both years indicated the presence of a primary disease gradient. At harvest, infected plants were segregated by treatment and by duration of infection [based on disease ratings taken from the time of first symptom expression (beginning at 110 to 120 days after transplanting and then every 5 to 10 days until harvest)]. Early-vs. late-infected plants had no significant effect on yield (bulb weight). However, symptom expression in terms of the number of days after planting was significantly correlated with a disease severity index. Amount of rot in bulbs from plants displaying their first symptoms only 1 to 2 days before harvest (late-season infection) was not significant from rot levels in control bulbs at harvest. However, at 4 weeks after harvest, onions from plants with late-season infections exhibited significantly more rot in storage compared to the control.

Several studies were undertaken in commercial nonirrigated `Hass' avocado orchards under the subhumid semiwarm subtropical climate of the state of Nayarit, Mexico, with the following objectives: 1) to determine the frequency and intensity of vegetative shoot flushes and their contribution to the production of floral shoots, 2) to quantify the effect of tree fruit load on the occurrence of vegetative shoot flushes during the year and the relationship between vegetative and reproductive shoot number during flowering, and 3) to determine the time when apical buds borne on the major vegetative shoot flushes reached irreversible commitment to flowering (floral determination) through the use of shoot defoliation and girdling. Data trees were selected in two orchards based on their current crop load. Four to five branches per tree were tagged, and the number and intensity of vegetative flushes that developed during 2 years, as well as the type of growth produced by apical buds of shoots of different ages, were recorded at the end of the winter bloom periods for two separate years, 1999 and 2001. In a separate experiment using a different set of trees, winter and summer flush shoots were defoliated (year 1) or defoliated and girdled (year 2) at different stages of bud development from September to January in each case. Four vegetative flushes occurred each year. The winter flush that emerged in Feb. 1998 made the greatest contribution to the 1999 winter bloom—76.5% of the shoots produced floral shoots. Contributions of the summer 1 (late July 1998), summer 2 (early Aug. 1998), and summer 3 (late Aug. 1998) flushes to flowering were intermediate. A total of 30.6%, 36.4%, and 19% of the shoots produced floral shoots respectively. All four vegetative flushes produced a similar number of vegetative shoots during winter bloom. Evaluation of the 2001 winter bloom for trees with high (>95 kg fruit/tree) and low (<70 kg fruit/tree) crops showed no effect of tree fruit load on the production of vegetative or floral shoots by winter or summer vegetative flushes. Irrespective of time of treatment (shoot defoliation and girdling) or shoot age, irreversible commitment to flowering of apical buds occurred by 15 Oct., and this stage was associated with an average of 27.5 chilling days (temperature, ≤19 °C) for both years. Buds irreversibly committed to flowering were closed and pointed, with partial senescence of bud scales. Anatomically, the buds showed a convex primary axis meristem and four secondary axis floral shoot meristems.

Colored shade nets may affect plant growth and fruit yield of horticultural crops. The understanding of how colored shade nets influence plants, however, is far from complete. The objective of this study was to determine the effects of colored shade nets on bell pepper fruit yield, postharvest transpiration, color, chemical composition, and antioxidant capacity. The experiment was conducted in Tifton, GA, during the spring of 2015 and 2016. The experimental design was a randomized complete block with four replications and five colored shade net treatments (black, red, silver, and white nets, and an unshaded control). The nets were placed on the top of wooden rectangular structures (15 m wide × 6 m long × 5 m high), leaving the sides of the structures uncovered. Results showed that in both 2015 and 2016, marketable and total fruit number, yield, and individual fruit weight were reduced under the unshaded treatment. There were inconsistent differences in marketable and total fruit number, yield, and individual fruit weight among colored shade nets. Postharvest fruit transpiration and skin permeance were also reduced in unshaded conditions, and no differences were found among colored shade nets. Fruit color L* and b* values were highest, and a* value was lowest in unshaded conditions. Fruit soluble solids, total phenols, flavonoids, and antioxidant capacity [Cupric Reducing Antioxidant Capacity (CUPRAC) and Trolox Equivalent Antioxidant Capacity (TEAC)] responded differently among colored shade nets in the 2 years. Total phenols, flavonoids, and TEAC, however, were among the highest in unshaded conditions. In conclusion, results of the present study support previous findings that shade nets increase fruit yield and quality in bell pepper compared with fruit produced in unshaded conditions. Nevertheless, there were no consistent differences in fruit total and marketable yield and postharvest fruit transpiration and chemical composition of fruit produced under colored shade nets.

Mulch is often applied in landscape planting beds for weed control, but little research has focused specifically on mulch and preemergence (PRE) herbicide combinations. The objectives of this research were to determine the efficacy of herbicide + mulch combinations and which factors significantly affected weed control, including herbicide formulation and posttreatment irrigation volumes. Additional objectives were to determine efficacy derived from mulch or herbicides used alone under herbicide + mulch combinations and to identify differences in the additive (herbicide + mulch combinations) or singular (herbicide or mulch) effects compared with the use of herbicides or mulch only. Large crabgrass (Digitaria sanguinalis), garden spurge (Euphorbia hirta), and eclipta (Eclipta prostrata) were used as bioassay species for prodiamine, dimethenamid-P + pendimethalin, and indaziflam efficacy, respectively. The experiment consisted of a factorial treatment arrangement of two herbicide formulations (granular or spray applied), three mulch types [hardwood chips (HWs), pine bark (PB), and pine straw (PS)], two mulch depths (1 and 2 inches), and three levels of one-time, posttreatment irrigation volumes (0.5, 1, and 2 inches). Three sets of controls were used: the first set included three mulch types applied at two depths receiving only 0.5-inch irrigation volume, the second set included only two herbicide formulations and three one-time irrigation volumes, whereas the last set received no treatment (no herbicide or mulch) and only 0.5-inch irrigation volume. High levels of large crabgrass and garden spurge control (88% to 100%) were observed with all herbicide + mulch combinations evaluated at mulch depths of 1 inch or greater. When comparing mulch types, the best eclipta control was achieved with hardwood at 2 inches depth. The spray formulation of indaziflam outperformed the granular formulation in most cases when used alone or in combination with mulch. Overall, the results showed that spray formulations of prodiamine and dimethenamid-P + pendimethalin were more effective than granular formulations when applied alone, whereas indaziflam was more effective as a spray formulation when used both alone and in combination with mulch. Increasing irrigation volume was not a significant factor for any of the herbicide + mulch combinations when evaluating overall weed control.

Field trials were conducted in two locations in Spain to determine the effect of methyl bromide (MBr) alternatives on soilborne diseases and nematodes, and strawberry (Fragaria ×ananassa) yields under high-tunnel conditions. Fumigant treatments were applied to the same plots each year. Treatments were MBr + chloropicrin (Pic) (50:50, v/v) at a rate of 400 kg·ha⁻¹; 1,3-dichloropropene (1,3-D) + Pic (65:35, v/v) at 300 kg·ha⁻¹; Pic at 300 kg·ha⁻¹; dimethyl disulfide (DMDS) + Pic (50:50, v/v) at 500 kg·ha⁻¹; propylene oxide at 550 kg·ha⁻¹; dazomet at 400 kg·ha⁻¹; and calcium cyanamide (Ca-cyanamide) at 700 kg·ha⁻¹. A nontreated control was also included. Fumigation with MBr + Pic, 1,3-D + Pic, Pic, and DMDS + Pic consistently improved early and total marketable strawberry yields in both locations. This response was caused by successful soilborne fungus and nematode control, improving strawberry growth and development, which resulted in increased plant canopy diameters and higher strawberry early and total yield.