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Xiaojie Zhao, Guihong Bi, Richard L. Harkess and Eugene K. Blythe

The form of nitrogen (N) in fertilizer can influence plant growth, nutrient uptake, and physiological processes in the plant. However, few studies have been conducted on the effects of N form on tall bearded (TB) iris (Iris germanica L.). In this study, five NH4:NO3 ratios (0:100, 25:75, 50:50, 75:25, and 100:0) were applied to investigate the response of TB iris to different N form ratios. NH4:NO3 ratios in fertilizer did not affect the leaf, root, and rhizome dry weight, or total plant dry weight. Plant height and SPAD reading were affected by NH4:NO3 ratios in some months, but not over the whole growing season. Neither spring nor fall flowering was influenced by NH4:NO3 ratios. Across the whole growing season, leachate pH was increased by higher NH4:NO3 ratios. At the end of the growing season, concentrations of phosphorous (P), iron (Fe), manganese (Mn), zinc (Zn), copper (Cu) in leaf; calcium (Ca), magnesium (Mg), Mn, boron (B) in root; and N, P, Mg, Fe, Mn, and Zn in rhizome tissues were affected by NH4:NO3 ratios. Greater NH4:NO3 ratios increased the uptake of Fe, Mn, and Zn. The net uptake of N was unaffected by NH4:NO3 ratios, which indicates TB iris may not have a preference for either ammonium or nitrate N.

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Eugene K. Blythe, Jeff L. Sibley and Ken M. Tilt

Stem cuttings of Hydrangea paniculata Sieb., Rosa L. `Red Cascade', Salvia leucantha Cav., and Solenostemon scutellarioides (L.) Codd `Roseo' were inserted into six rooting substrates: monolithic slag [(MgFe)2Al4Si5O18], sand, perlite, vermiculite, Fafard 3B, or fine pine bark. Rooting, initial shoot growth, and ease of dislodging substrate particles from root systems upon bare-rooting by shaking and washing cuttings rooted in monolithic slag were compared to cuttings rooted in the five other substrates. Rooting percentage, number of primary roots per rooted cutting, and total root length per rooted cutting for cuttings rooted in monolithic slag were generally similar to the five other substrates. Particles of monolithic slag were dislodged more readily from root systems by shaking than were the other substrates. Gentle washing removed almost all particles of monolithic slag and sand from the root systems of all taxa and removed almost all particles of pine bark from all taxa except S. scutellarioides `Roseo'. Monolithic slag had a bulk density similar to sand, retained less water than the other substrates, and was similar to perlite, vermiculite, and pine bark in particle size distribution. Our studies indicate that monolithic slag, where regionally available, could provide a viable material for producing bare-root cuttings.

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Xiaojie Zhao, Guihong Bi, Richard L. Harkess, Jac J. Varco and Eugene K. Blythe

This study investigated how spring nitrogen (N) application affects N uptake and growth performance in tall bearded (TB) iris ‘Immortality’ (Iris germanica L.). Container-grown iris plants were treated with 0, 5, 10, 15, or 20 mm N from 15NH4 15NO3 through fertigation using a modified Hoagland’s solution twice a week for 6 weeks in Spring 2013. Increasing N rate increased plant height, total plant dry weight (DW), and N content. Total N content was closely related to total plant DW. The allocation of N to different tissues followed a similar trend as the allocation of DW. In leaves, roots, and rhizomes, increasing N rate increased N uptake and decreased carbon (C) to N ratio (C/N ratio). Leaves were the major sink for N derived from fertilizer (NDFF). As N supply increased, DW accumulation in leaves increased, whereas DW accumulation in roots and rhizomes was unchanged. This indicates increasing N rate contributed more to leaf growth in spring. Nitrogen uptake efficiency (NupE) had a quadratic relationship with increasing N rate and was highest in the 10 mm N treatment, which indicates 10 mm was the optimal N rate for improving NupE in this study.

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Eugene K. Blythe, Jeff L. Sibley, Ken M. Tilt and John M. Ruter

In five experiments, singlenode cuttings of `Red Cascade' miniature rose (Rosa) were treated with a basal quick-dip (prior to insertion into the rooting substrate) or sprayed to the drip point with a single foliar application (after insertion) of Dip `N Grow [indole-3-butyric acid (IBA) + 1-naphthaleneacetic acid (NAA)], the potassium salt of indole-3-butyric acid (K-IBA), or the potassium salt of 1-naphthaleneacetic acid (K-NAA); a single foliar spray application of Dip `N Grow with and without Kinetic surfactant; or multiple foliar spray applications of Dip `N Grow. Spray treatments were compared with their respective basal quick-dip controls {4920.4 μm [1000 mg·L-1 (ppm)] IBA + 2685.2 μm (500 mg·L-1) NAA, 4144.2 μm (1000 mg·L-1) K-IBA, or 4458.3 μm (1000 mg·L-1) K-NAA}. Cuttings sprayed with 0 to 246.0 μm (50 mg·L-1) IBA + 134.3 μm (25 mg·L-1) NAA, 0 to 207.2 μm (50 mg·L-1) K-IBA, or 0 to 222.9 μm (50 mg·L-1) K-NAA resulted in rooting percentages, total root length, percent rooted cuttings with shoots, and shoot length similar to or less than control cuttings. Exceptions were cuttings sprayed with 0 to 2.23 μm

(0.5 mg·L-1) K-NAA, which exhibited shoot length greater than the control cuttings. Addition of 1.0 mL·L-1 (1000 ppm) Kinetic organosilicone surfactant to spray treatments resulted in greater total root length and shoot length. Repeated sprays (daily up to seven consecutive days) had no or negative effects on root and shoot development.

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Christian M. Baldwin, Eugene K. Blythe, A. Douglas Brede, Jami J. Mayer and R. Golembiewski

The use of glyphosate-tolerant perennial ryegrass (Lolium perenne L.) (PRG) cultivars JS501 and Replay provides turfgrass managers a unique option for annual bluegrass (Poa annua L.) (ABG) control. Both cultivars can tolerate a maximum glyphosate rate of 0.81 kg·ha−1 acid equivalent (a.e.) after establishment under optimal growing temperatures (16 to 24 °C). However, tolerance to applications made immediately after germination and during low air temperatures has received limited investigation. Therefore, objectives of this research were to determine the seedling tolerance and low-temperature response after a fall season glyphosate application to both cultivars. Field trials were conducted in Idaho and Oregon. For the fall application response trial in Idaho, glyphosate was applied at 0, 0.15, 0.29, 0.58, 1.16, 1.74, 2.32, and 3.48 kg·ha−1 a.e. In Oregon, glyphosate was applied at 0, 0.15, 0.29, 0.44, 0.58, 1.16, and 3.48 kg·ha−1 a.e. At both sites, applications were made between late September and early October. To determine seedling tolerance, both cultivars were sprayed with glyphosate at the one-leaf stage (LS), two LS, three LS, or four LS at rates of 0, 0.15, 0.29, and 0.58 kg·ha−1 a.e. Across all trials, ratings included PRG color, cover, and injury. At both trial locations, regression analysis revealed a rate of ≈0.27 kg·ha−1 a.e. was required to cause 20% leaf firing in the fall application response trial. In the seedling tolerance trial, glyphosate applied at 0.58 kg·ha−1 a.e. at the one LS, two LS, and three LS had color ratings 8.0 or greater; however, color ratings dropped to 4.6 when an application was made at the four LS. Based on the environmental conditions of each trial, results suggest glyphosate applications greater than 0.27 kg·ha−1 a.e. as minimum air temperatures approach 0 °C should be avoided. Also, applications should be avoided at the three to four LS if the application rate is greater than 0.29 kg·ha−1 a.e.

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Julie P. Newman, Joseph P. Albano, Donald J. Merhaut and Eugene K. Blythe

Release characteristics of four different polymer-coated fertilizers (Multicote, Nutricote, Osmocote, and Polyon) were studied over a 47-week period in a simulated outdoor, containerized plant production system. The 2.4-L containers, filled with high-fertility, neutral-pH substrate, were placed on benches outdoors to simulate the environmental conditions often used for sun-tolerant, woody perennials grown in the southwestern United States. Container leachates were collected weekly and monitored for electrical conductivity, pH, and concentrations of NH4 +N, NO3 N, total P, and total K. Concentrations of most nutrients in leachates were relatively high, but fluctuated frequently during the first third of the study period, and then gradually decreased and stabilized during the last 27 weeks. Osmocote often resulted in greater NH4 + and total inorganic N concentrations in leachates than other fertilizers during weeks 1 through 5, whereas Multicote produced higher NH4 + in leachates than most of the other fertilizer types during weeks 9 through 12. Overall, total P concentrations were greater with Multicote during a third of the experimental period, especially when compared with Osmocote and Polyon. Differences were also observed among treatments for leachate concentrations of K, with Polyon and Multicote fertilizers producing greater K concentrations in leachates compared with Osmocote during several weeks throughout the experimental period. Leachate concentrations of NO3 N and P from all fertilizer types were usually high, especially from week 5 through week 30.

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Joseph P. Albano, Donald J. Merhaut, Eugene K. Blythe and Julie P. Newman

Nutrient release characteristics of four different controlled-release fertilizers (Osmocote, Nutricote, Polyon, and Multicote) were monitored during an 11-month period in a simulated outdoor nursery production facility. Although no plants were used in the experiment, fertilization rates, irrigation regimes, and cultural practices simulated those typically used to produce fast-growing, high-nutrient-requiring containerized woody ornamentals. Fertilizer prill release characteristics were monitored through analyses of leachates, which were collected weekly. Concentrations of Mg, Mn, Zn, Cu, and Mo were relatively high during the first 5 to 10 weeks of the experiment, then declined and usually stabilized during the remainder of the study. However, Mn and Zn displayed erratic increases in concentrations several times throughout the study. Calcium concentrations did not increase until the fifth week, rapidly peaked to about 300 mg·L–1, and then decreased and leveled off to ≈80 to 100 mg·L–1 during the remainder of the study. Several significant differences were observed between treatments. The Osmocote treatment had significantly greater Ca and Mg concentrations in the leachate than the other fertilizer types during the last 6 weeks of the study, whereas the Nutricote treatment often had significantly greater Fe concentrations than leachates from other treatments, especially during the last 26 to 35 weeks of the study, and significantly greater Zn concentrations than the other CRFs during the last 21 weeks of the study. Based upon U.S. Environmental Protection Agency guidelines, concentrations of Fe were often more than the allowable limit of 0.3 mg·L–1 with all fertilizer types, but especially with Nutricote. Concentrations of Mn and Cu also exceeded federal guidelines, particularly during the first several weeks of the study.

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Donald J. Merhaut, Eugene K. Blythe, Julie P. Newman and Joseph P. Albano

Release characteristics of four types of controlled-release fertilizers (Osmocote, Nutricote, Polyon, and Multicote) were studied during a 47-week simulated plant production cycle. The 2.4-L containers containing a low-fertility, acid-based substrate were placed in an unheated greenhouse and subjected to environmental conditions often used for production of azaleas and camellias. Leachate from containers was collected weekly and monitored for pH, electrical conductivity, and concentrations of NH4 + N, NO3 N, total P and total K. Leachate concentrations of all nutrients were relatively high during the first 10 to 20 weeks of the study, and then gradually decreased during the remaining portion of the experiment. Differences were observed among fertilizer types, with Multicote often resulting in higher concentrations of N, P, and K in leachates compared to the leachates from the other fertilizer types during the first half of the study. Concentrations of NO3 and P from all fertilizer types were often above permissible levels as cited in the federal Clean Water Act.

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Eugene K. Blythe, Donald J. Merhaut, Julie P. Newman and Joseph P. Albano

Leachate from containerized substrate containing one of four different controlled-release fertilizers (Osmocote, Nutricote, Polyon, or Multicote) were monitored for concentrations of Ca, Mg, Fe, Mn, Zn, Cu, and Mo during a 47-week period. Environmental and cultural practices simulated an unheated greenhouse production program typically used for low-nutrient-requiring crops such as azalea and camellia. Leachate concentrations of all nutrients were relatively high during the first 10 to 20 weeks of the study, and then gradually decreased during the remaining portion of the experiment. Few differences were observed among fertilizer types. Of the elements monitored, only Fe and Mn leachate concentrations were above critical levels specified in the Clean Water Act by the U.S. EPA.

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Tongyin Li, Guihong Bi, Richard L. Harkess, Geoffrey C. Denny, Eugene K. Blythe and Xiaojie Zhao

One-year-old liners of Encore® azalea ‘Chiffon’ (Rhododendron sp.) were transplanted in Apr. 2013 into two types of one-gallon containers: black plastic container and paper biodegradable container. Azalea plants were fertilized with 250 mL of nitrogen (N) free fertilizer solution twice weekly plus N rate of 0, 5, 10, 15, or 20 mm from ammonium nitrate (NH4NO3). All plants were irrigated with the same total volume of water through one or two irrigations daily. Plant growth and N uptake in response to N fertilization, irrigation frequency, and container type were investigated. The feasibility of biodegradable paper containers was evaluated in 1-year production of Encore® azalea ‘Chiffon’. Paper biocontainers resulted in increased plant growth index (PGI), dry weights (leaf, stem, root, and total plant dry weight), leaf area, and root growth (root length and surface area) compared with plastic containers using N rates from 10 to 20 mm. Biocontainer-grown plant had more than twice of root length and surface area as plastic container–grown plant. Leaf SPAD reading increased with increasing N rate from 0 to 20 mm. One irrigation per day resulted in greater PGI, root dry weight, root length, root surface area, and root N content than two irrigations per day. Higher tissue N concentration was found in plants grown in plastic containers compared with those grown in biocontainers when fertilized with 15 or 20 mm N. However, N content was greater for plants grown in biocontainers, resulting from greater plant dry weight. The combinations of plastic container and one irrigation per day and that of 20 mm N and one irrigation per day resulted in best flower production, 21.9 and 32.2 flowers per plant, respectively. Biocontainers resulted in superior vegetative growth of azalea plant compared with plastic containers with sufficient N supply of 10, 15, and 20 mm.