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  • Author or Editor: Eric E. Roos x
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Seeds of the recalcitrant species Litch i chinénis and Euphoria longan were stored in humid conditions at 8-10C under three different atmospheres: air, 80% nitrous oxide (N20)/20 % oxygen, and 100% nitrous oxide. The combination of anesthetic and oxygen extended storage longevity of both species. Oxygen was required for maintenance of viability; seeds stored under 100% N20 lost germinability at the most rapid rate. Lychee seeds retained 92% of control germination after 12 weeks under 80% N20/20% 02, while those under air lost 56% viability. Longan seeds lost all viability after 7 weeks under air, yet retained 70% of their control germination under 80% N20/20% 02. The combination of anesthetic and oxygen atmospheres could provide a new approach to recalcitrant seed storage.

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Abstract

Garden bean seeds stored under high temperature (32.2°C) and relative humidity (90%) for periods up to 15 weeks showed a drop in germination after 9 weeks with the standard laboratory germination test while a recently developed vigor test for beans (Epicotyl wt measurement) showed a loss in vigor after only 3 weeks of storage. We conclude that this new testing procedure can be used to measure loss of vigor in bean seed stored under unfavorable conditions before a loss of viability is detected by the standard germination test.

Open Access

Abstract

Previous research showed a relationship between viability and electrolyte leakage; here, we develop an index of viability based on electrolyte leakage from sample populations of seeds soaked in deionized distilled water. Conductivity of leachates from individual seeds was determined for 10 lots of lettuce (Lactuca sativa L.), each germinating at 99%. Conductivity data for two lots of soybean (Glycine max L.) seeds germinating at 100% and 74%, respectively, were obtained from literature. Cumulative frequency distributions (CFD) with a class interval of one µA, were fitted with a natural logarithmic form of the Richards function, which requires no arbitrary starting values. The procedure provided an effective estimation of slopes [(dCF/dµA)MAX] of hypothetical lines tangent to inflection points of the respective sigmoidal CFD curves. We suggest that this maximum slope, or internal slope can be used as a seed viability index. The index is unaffected by outlier µA readings and reflects the shape of the CFD. It is also a measure of seed-to-seed variability in leachate conductivity. The se of the 10 internal slopes derived from the 10 lettuce seed lots was 3.8. The viability index is sensitive, since nearly a four-fold difference in internal slope was found for the two soybean seed lots. The greater the internal slope, the less the variation among individual seed conductivities and the higher the seed quality.

Open Access