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Hong Zhu, Rongcai Yuan, Duane W. Greene, and Eric P. Beers

qPCR was performed using the Power SYBR Green PCR Master Mix Kit (Applied Biosystems) on an Applied Biosystems 7500 Real-Time PCR System according to the manufacturer's instructions. Gene-specific primers were designed for non-conserved areas using

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Jiaqi Hu, Hye-Ji Kim, Houbin Chen, and Biyan Zhou

predict the LcSVP s tertiary structure. Table 1. Primer sequences for cloning and quantitative real-time polymerase chain reaction (qRT-PCR). Quantitative real-time polymerase chain reaction (RT-PCR) analysis. First-strand cDNA was synthetized by using

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John R. Stommel, Gordon J. Lightbourn, Brenda S. Winkel, and Robert J. Griesbach

using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Real-time polymerase chain reaction (PCR) was used to compare flavonoid gene expression ( Chs , Dfr , Ans , Myb A , Myc , and Wd ) between anthocyanin-pigmented and

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Huai-Fu Fan, Wen Chen, Zhou Yu, and Chang-Xia Du

tree files were viewed and edited by MEGA 3.1 software. Quantitative real-time PCR (qRT-PCR) analysis. To understand the effects of salt stress on CsaNAPOD expression, we treated cucumber seedlings with 50 m m NaCl stress for 96 h. The expression of

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Jure Kolarič, Irena Mavrič Pleško, Stanislav Tojnko, and Matej Stopar

the ABI 7500 Real-Time PCR System (Applied Biosystems by Life Technologies, Carlsbad, CA) with the following cycling parameters: 95 °C for 10 min and 40 cycles of 94 °C for 30 s, 62 °C for 30 s, and 72 °C for 40 s. At the end, dissociation curves were

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Carl E. Sams, Dilip R. Panthee, Craig S. Charron, Dean A. Kopsell, and Joshua S. Yuan

amplification. Real-time PCR as described by Yuan et al. (2006) was performed on a subset of 14 differentially expressed genes associated with GS, carotenoids, S-metabolism, and plant defense-related biomolecules with two replications. For real-time PCR

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An Qin, Xiaosan Huang, Huping Zhang, Juyou Wu, Jie Yang, and Shaoling Zhang

for 1 h and quantified at 525 nm. A standard curve of AsA was established and used for quantification. Expression analysis by quantitative real-time PCR. The genes involved in the tomato AsA-GSH cycle from the WT and transgenic line plants before and

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Keun H. Cho, Joo Young Kim, Maria I. Alvarez, Veronica Y. Laux, Lauren K. Valad, Joshua M. Tester, Thomas A. Colquhoun, and David G. Clark

. Ell, J. Strano, M.S. 2017 A nanobionic light-emitting plant Nano Lett. 17 7951 7961 Livak, K.J. Schmittgen, T.D. 2001 Analysis of relative gene expression data using real-time quantitative PCR and the 2 −ΔΔCT method Methods 25 402 408 Malik, K. Wu, K

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Tracie K. Matsumoto, Francis T.P. Zee, Jon Y. Suzuki, Savarni Tripathi, James Carr, and Bruce Mackey

. Scholdberg, T.A. Burton, D.D. Norden, T.D. Shokere, L.A. Jenkins, G.R. 2007 Evaluating homogeneity of LL601 rice in commercial lots using quantitative real-time PCR J. Agr. Food Chem. 55 6060 6066

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Xiaojuan Zong, Jiawei Wang, Li Xu, Hairong Wei, Xin Chen, Dongzi Zhu, Yue Tan, and Qingzhong Liu

analyses of the PcMPKs were generated from the alignments of the cDNA and genomic DNA amplicon sequences. Real-time qRT-PCR analysis. Primers for real-time qRT-PCR analysis were designed using Beacon Designer 8 software (Premier Biosoft) ( Supplemental