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of this work, we also investigated the effects of the original explant source and the light environment on the rate of shoot organogenesis from callus cultures. Materials and Methods Plant material. Open-pollinated, immature seeds (≈40

of fruit set (3–6 weeks after transplanting) among the 2012, 2013, and 2014 triploid watermelon evaluations. The x-axis represents the estimated number of days of pollination. In 2012, diploid flowers opened on ≈8 June (climate data shown from 8 June

cultivars except Sweetheart and Staccato ® . This included ‘Sovereign™’, ‘Sentennial™’, and ‘SPC243’, which are also open-pollinated ‘Sweetheart’ progeny and were expected to pose a similar challenge for discrimination. Finally, a broader range of SSR

polymorphic when considering the eight control dark violet to black-fruited varieties represented by modern hybrids, older open pollinated varieties and landraces. SSR profiles demonstrated that ‘Black Beauty’ and E13GB42 were distinct from the other dark

provenance–progeny test series. Materials and Methods Seedlings were grown from open-pollinated seed collected during the 1994 range-wide cone collection. Progeny from 188 of these trees from the following six seed sources were included in this study

cytotypes. Materials and Methods Plant material. Open-pollinated fruit were collected from one P. lusitanica on the Oregon State University campus (lat. 44°34′04″N, long. 123°17′14″W) in Corvallis, OR, on 5 Sept. 2014. The exocarp and mesocarp were removed

muscadine seed and lower the lengthy stratification periods now used. Materials and Methods Plant material. Open-pollinated seeds of ‘Fry’ muscadine were used for all treatments. Fully mature ‘Fry’ berries were collected from vines located at

to produce a fertile amphidiploid (HHCC, 2n = 4x = 38) via somaclonal variation during in vitro embryo culture ( Chen et al., 1998 ). This amphidiploid was subsequently self-pollinated for several generations, resulting in fertile germplasm that was

distributed and cultivated worldwide, the genetic diversity and genetic relationship using DNA analysis are poorly described in China. In this study, open-pollinated, hybrid and inbred lines were included for most of the ecotypes and are hereafter collectively

), Thailand. Site selection was based on the ability to conduct an effective bioassay, rather than on nearby production or viral prevalence. Capsicum entries (98) comprising breeding lines, open pollinated varieties, genebank accessions, and wild species