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Paolo Boccacci, Roberto Botta, and Mercè Rovira

cultivars due to their high discriminating power at a relatively low cost. Among the available DNA markers, microsatellite or simple sequence repeat (SSR) markers appear to be best-suited to cultivar fingerprinting. They are generally codominant, highly

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Kang Hee Cho, Seo Jun Park, Su Jin Kim, Se Hee Kim, Han Chan Lee, Mi Young Kim, and Jae An Chun

fragment length polymorphisms, single sequence repeats (SSRs), and inter simple sequence repeats (ISSRs) for DNA fingerprinting. The choice of a DNA marker depends on the scale and purpose of cultivar identification; several different DNA marker types have

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P. Escribano, M.A. Viruel, and J.I. Hormaza

). Microsatellites or SSRs have become the markers of choice for fingerprinting purposes in most plant species ( Gupta and Varshney, 2000 ), due to their high polymorphism, codominance, and reproducibility. Recently, a set of SSRs was reported for the first time in

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Xiao-li Li and Yong He

. MLR model 11 was statistically significant at 99% confidence level with three significant variables, x 3 , x 9 , and x 10 ( Table 6 ). The three corresponding wavelengths, 488, 695, and 931 nm, were considered as the characteristic fingerprint spectra

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Ghazal Baziar, Moslem Jafari, Mansoureh Sadat Sharifi Noori, and Samira Samarfard

et al., 2014 ; Saddoud et al., 2005 ). However, microsatellite markers are locus-specific, thus requiring extensive genetic research ( Agarwal et al., 2008 ). In the present study, RAPD fingerprinting method was employed for its efficiency in

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R.N. Trigiano, M.C. Scott, and G. Caetano-Anollés

Four chrysanthemum (Dendranthema grandiflora) spontaneous and radiation-induced sports from the cultivar `Charm' and phenotypically differing only in flower color were individually characterized using arbitrary signatures from amplification profiles (ASAP). ASAP analysis is based on a two-step arbitrary primer amplification procedure that produces “fi ngerprints of fingerprints.” In the first step, `Charm', `Dark Charm', `Dark Bronze Charm', `Salmon Charm', and `Coral Charm' were fingerprinted by DNA amplification fingerprinting (DAF) with standard octamer arbitrary primers. Diluted products from three monomorphic fingerprints for each cultivar were subsequently reamplified using four minihairpin decamer primers. Each of the 12 ASAP profiles revealed polymorphic loci that were used to uniquely identify cultivars and estimate genetic relationships. The ASAP technique permits identification of previously genetically indistinguishable plant material and should facilitate marker assisted breeding and protection of ownership rights.

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Kang Hee Cho, Jung Ho Noh, Seo Jun Park, Se Hee Kim, Dae-Hyun Kim, and Jae An Chun

relationships ( Castro et al., 2012 ; Guo et al., 2012 ; This et al., 2004 ). Simple sequence repeats (SSR) markers provide excellent DNA fingerprinting methods because of their codominant Mendelian inheritance, high degree of polymorphisms, and abundance in

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Wanmei Jin, Qiang Zhang, Sunzhong Liu, Qinping Wei, Wanmei Jin, Zongming Cheng, Xiaohui Xue, and Tingzhen Yang

., 2008 ; Liebhard et al., 2002 ; Silfverberg-Dilworth et al., 2006 ). SSR markers have been widely used in apple for cultivar fingerprinting, characterization of the genetic diversity, and preservation of core genetic resources ( Iannaccone et al., 2007

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Joseph C. Kuhl and Veronica L. DeBoer

. 1995 AFLP: A new technique for DNA fingerprinting Nucleic Acids Res. 23 4407 4414 Walkey, D.G.A. Mathews, K.A.M. 1979 Rapid cloning propagation of rhubarb ( Rheum rhaponticum ) from meristem-tips in tissue

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Briana L. Gross, Gayle M. Volk, Christopher M. Richards, Philip L. Forsline, Gennaro Fazio, and C. Thomas Chao

fingerprinting of four apple clones by S-SAP analysis J. Hort. Sci. Biotechnol. 85 53 58 Taberlet, P. Luikart, G. 1999 Non-invasive genetic sampling and individual identification Biol. J. Linn. Soc. Lond. 68 41 55 Tessier, C. David, J. This, P. Boursiquot, J