In order to investigate their relationships with brown bead, a data set composed of 48 variables characterizing the developmental rate, climate, and nutrients in the soil and in the tissues of heads of broccoli (Brassica oleracea L., Italica group) was collected from 328 plots (41 experimental fields over 3 year× 4 N fertilization level× 2 blocks). The four N treatments were 85-0-0, 85-54-0, 85-54-54, and 85-54-108, the first number indicating the N level (kg·ha-1) applied before planting; the second, N level applied 5 weeks after planting; and the last, N level applied 7 weeks after planting. Broccoli plants were either direct-seeded (26 experimental fields) or transplanted (15 experimental fields). Whether direct-seeded or transplanted, fast-developing broccoli plants showed a lower incidence of brown bead. More particularly, heads of transplanted broccoli plants experiencing warmer temperatures had a lower brown bead incidence and severity. A regular supply of water decreased the incidence and severity of the physiological disorder in both direct-seeded and transplanted broccoli plants. Low levels of Ca and high levels of Mg and K in mature broccoli head tissues were associated with a higher incidence of brown bead. Multiple-regression models were developed to predict the percentage of broccoli heads with brown bead for direct-seeded plants (R 2 = 0.76; n = 104), and for transplanted plants (R 2 = 0.69; n = 44). For direct-seeded broccoli, solar radiation between the button stage (head diameter of 2.5 cm) and maturity (head diameter of 10 cm), as well as soil and tissue Mg content, were among the first variables to enter the regression models. In general, more solar radiation and less precipitation translated into more heads showing brown bead symptoms. For transplanted broccoli plants, the minimum temperature from the button stage to maturity was a key variable in the prediction of the percentage of heads with brown bead and the corresponding index of severity.
Sylvie Jenni, Pierre Dutilleul, Stephen Yamasaki, and Nicolas Tremblay
Marshall K. Elson, Ronald D. Morse, Dale D. Wolf, and David H. Vaughan
High summer temperatures may reduce plant stands of direct-seeded fall broccoli (Brassica oleracea var. italica Plenck). The influence of constant and diurnally alternating temperatures in the range of 5 to 42C on germination and emergence of `Packman' broccoli was evaluated. Germination was defined as protrusion of the radicle from the seedcoat, and emergence as 10 mm elongation of the radicle. The range of constant temperatures from 10 to 30C for 14 days was satisfactory for 90% germination and 75% emergence. However, alternating temperatures extended the acceptable emergence range to 5/17 through 20/32C. Since soil temperatures in warm climates often exceed 20/32C during the summer, high-temperature inhibition of seed germination and seedling emergence is a potentially important factor limiting direct-seeded broccoli stands.
Roland Roberts, David Bender, and Samuel Field
Extension-research teamwork supports Texas High Plains onion grower-shippers in transition from unprofitable labor intensive marketing and culture to profitable mechanical systems that are less stressful to workers. System comparisons include machine harvest vs. lifting and hand clipping; stationary seed grading and bagging vs. mobile field grading and bagging; transplant vs. fall seeding, spring seeding and dry set production. Old marketing systems cost growers $4.30/50-lb. sack, and the innovative system costs $2.59 to $3.00/sack. Old transplant systems average $450 to $500/acre and direct seeding costs $200/acre. Net increase in return to grower management from adoption of new systems range from $1,300 to $1,700. Extension and research conduct economic analysis, cultivar performance trials, seeding technique studies and on-farm demonstrations.
Oyette L. Chambliss, Arthur G. Hunter, and Richard O. Hampton
Germplasm accessions and advanced lines were evaluated for seed transmission of the seed borne viruses, cucumber mosaic (CMV), cowpea severe mosaic (CSMV), and blackeye cowpea mosaic (B1CMV). Seed samples from 822 field plots (274 out of 300 accessions in 3 replications) which had been evaluated for insect resistance in 1992 were planted in the greenhouse. Mosaic virus symptoms had been apparent throughout the 1992 field planting. Evaluation for mosaic symptoms was done at the seedling stage in the greenhouse and 89 entries exhibited seed borne virus symptoms in one or more plants. Of these, 78 were shown by ELISA (enzyme linked immunosorbant assay, direct antigen coating method) to contain at least one of the seed borne viruses for which we were evaluating.
Janine G. Haynes, Wallace G. Pill, and Thomas A. Evans
Switchgrass (Panicum virgatum L.) is one of the perennial, native, warm-season grasses recommended as a component of wildflower meadows. Seed treatments to alleviate low seed vigor and seed dormancy of switchgrass would enhance establishment of either plug transplants or seedlings after direct sowing into the meadow. “Heavy” seeds (45.5 mg/50 seeds) of open-pollinated switchgrass stored under cool and dry conditions (average 13 °C, 30% relative humidity) for 24 months had higher germination percentage than “light” seeds (26.0 mg per 50 seeds). In factorial combination, the heavy seeds were subjected to acid scarification (8 M H2SO4 for 5 min), sodium hypochlorite treatment (5.25% NaOCl for 15 min), and moist chilling (prechilling in 0.2% KNO3, for 14 days). Acid scarification followed by NaOCl treatment additively increased germination, a response that was associated with marked corrosion of the lemma margin in the distal region of the caryopsis, as observed by scanning electron microscopy. Prechilling the seeds following acid scarification and NaOCl further increased germination. All three treatments combined (acid scarification, NaOCl, and prechilling) almost doubled the final emergence and greatly increased seedling shoot dry mass in both a warm and cool postsowing environment. However, the effectiveness of these seed treatments was lost after 32 months of dry storage.
Dennis M. TeKrony and Dennis B. Egli
Both seed viability and vigor directly affect the performance of seeds planted to regenerate the crop. Although seed quality can influence many aspects of performance (e.g., total emergence, rate of emergence), this presentation will primarily examine the relationship of seed vigor to one aspect of performance - crop yield. Reductions in yield can be indirectly related to low seed vigor if the low vigor seed results in plant populations that are below a critical level. Thus, we investigated the direct effects of seed vigor on yield in the absence of population differences for annual crops that are harvested at three stages; during vegetative growth, early reproductive growth or at full reproductive maturity. Seed vigor affects vegetative growth and is frequently related to yield in crops that are harvested during vegetative growth or during early reproductive growth. However, there is usually no relationship between vigor and yield in crops harvested at full reproductive maturity because seed yields at full reproductive maturity are usually not closely associated with vegetative growth. The use of high vigor planting seed can be justified for all crops; however, to insure adequate plant populations over the wide range of field conditions which occur during emergence.
J.E. Warren and M.A. Bennett
Improved germination under unfavorable soil conditions is an important safeguard against yield losses in direct-seeded crops. Osmoprimed seed has been shown to provide earlier and more uniform germination as well as improve low temperature germination. These attributes combined with the reduced rates of damping-off associated with Pseudomonas aureofaciens AB254 creates a bioosmopriming seed treatment that provides rapid germination under a wider range of soil temperatures while exhibiting the disease resistance and improved growth associated with bacterial coatings. The objective of this work is to combine biopriming and osmopriming into one procedure, thus creating an environment for adequate seed hydration and rapid multiplication of beneficial bacteria which will thoroughly colonize the seed surface. Processing tomato seeds (Lycopersicon esculentum Mill. `OH8245') were bio-osmoprimed in aerated –0.8 MPa NaNO3 at 20°C for 4 days. On the fourth day, a mixture of nutrient broth, a defoaming agent, and bacteria that have been adjusted to the same osmotic potential is added. This is done so that the removal of seeds from the tank at the end of the 7-day treatment coincides with peak populations of bacteria. Pseudomonas aureofaciens AB254 multiplies very rapidly in this environment, with colony forming units for tomato averaging 4 × 105/seed. Results will also be reported for cucumber seed (Cucumis sativus L. `Score'), which were treated using a similar procedure. Bacterial populations per seed, germination characteristics and pathogen control will be discussed.
Daniel I. Leskovar, Daniel J. Cantliffe, and Peter J. Stoffella
`Sunny' tomato (Lycopersicon esculentum Mill.) containerized transplants were grown with the standard or conventional systems (SS) and with recently developed flotation systems (FS). Standard system and FS transplants, and direct-seeding using coated seeds were evaluated in the field for root and shoot growth and yield at Parrish, Bradenton, and Naples during fall, winter, and spring plantings. Plant growth characteristics were measured weekly before, during, and after transplanting or sowing. In the Parrish and Bradenton Fall 1987 and Bradenton Spring 1988 experiments, SS transplants had greater leaf area, root volume, shoot dry weights, and shoot: root ratios than FS transplants. During early development, the FS transplants had more lateral root growth than SS transplants, but had similar total root growth and horizontal and vertical root distribution after transplanting in the field. Transplants and direct-seeded plants allocated 72% of the total root mass in the upper 0 to 10 cm of the soil. In Fall 1987, SS transplants had between 29% and 41% more fruit yield than FS transplants at Bradenton and Parrish, respectively. In the Naples Winter 1988 and Parrish and Bradenton Fall 1989 experiments, both transplant types had similar fruit yields, but more than direct-seeded plants. Transplants grown with the flotation system are recommended for use provided that seedlings are grown and maintained with minimum hardening before establishment in the field.
Troy A. Larsen* and Christopher S. Cramer
New Mexico onion production will begin using mechanical harvesters in the near future in order to stay competitive in today's market. Past onion breeding objectives have focused on improving onions for hand harvesting instead of mechanical harvesting. Our breeding program is starting to evaluate germplasm for bulb firmness. The objectives of this study were to evaluate hybrid lines for their bulb firmness, to compare two methods of measuring bulb firmness, and to compare bulb firmness using two different production schemes. Bulb firmness of spring-transplanted and spring-seeded intermediate-day hybrid breeding lines was measured using a digital FFF-series durometer and a subjective rating of firmness achieved by squeezing bulbs. Bulbs were rated on a scale of 1 (soft) to 9 (hard). In general, these hybrid lines produced very firm to hard onions whether the lines were transplanted or direct-seeded. Bulb firmness of these lines measured with the durometer was greater when the lines were direct-seeded (74.9) than when transplanted (73.5). Conversely, when firmness was measured with our subjective rating, transplanted onions exhibited slightly greater firmness (8.9) than direct-seeded onions (8.8). For both transplanted and direct-seeded onions, durometer readings were weakly correlated in a positive fashion with our subjective rating. In general, durometer readings gave a greater spread in firmness measurements with a range of 69.6 to 77.8 in firmness values. Subjective ratings of bulb firmness ranged from 8.5 to 9.0. Depending on the firmness of evaluated breeding lines, our subjective rating system should be adjusted to better distinguish firmness differences between bulbs.
S.B. Sterrett, C.W. Coale Jr., and C.P. Savage Jr.
1 Assistant Professor. 2 Professor, Dept. of Agricultural Economics, Blacksburg campus. 3 Research Scientist A. Seed was kindly provided by Asgrow Seed Co., Kalamazoo, Mich., and by Peto Seed, Saticoy, Calif. The cost of publishing this