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Soon O. Park* and Kevin M. Crosby

Mature fruit size and shape are important traits of most melon types. Our objective was to identify RAPD markers associated with major QTL affecting fruit weight, length, diameter and shape by means of bulked segregant analysis in an F2 population from the ananas melon cross of Deltex (larger fruit size) × TGR1551 (smaller fruit size). Clear separations for fruit weight, length, diameter, and shape between Deltex and TGR1551 were observed. Continuous distributions for fruit weight, length, diameter and shape were found in the F2 population indicating quantitative inheritance for the fruit traits. Significant positive correlations were detected between fruit weight and shape traits (r = 0.73 to 0.80). A significant positive correlation was observed between fruit weight and glucose (r = 0.35) or fructose (r = 0.25), whereas no correlation was noted between fruit weight and sucrose or total soluble solids. Two small and large bulks for fruit weight and shape were developed from F2 plants. A total of 240 primers were used to simultaneously screen between the small and large bulks, and between Deltex and TGR1551. Twenty-six RAPD markers were polymorphic for the small and large bulks. Ten markers were found to be significantly and consistently associated with fruit size and shape traits on the basis of simple linear regression. Of the 10 markers associated, four displayed an amplified DNA fragment in the small bulk, while six showed an amplified DNA fragment in the large bulk. The associated marker OJ07.350 explained 15% to 27% of the phenotypic variation for the fruit traits. These markers associated with QTL for melon fruit size and shape are expected to be useful in melon breeding programs for modifying fruit size.

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D.C. Ferree and J.G. Streeter

Container-grown `Chambourcin' grapevines were exposed to soil compaction created by changing soil bulk density to determine the effect of levels of compaction, rootstocks and moisture stress on mineral nutrition, leaf gas exchange and foliar carbohydrate levels. Shoot growth, leaf area, number of inflorescences and leaf dry weight decreased linearly as soil bulk density increased with the effects being significant above 1.4 g·cm-3. The early season leaf area was reduced 40% in the second season, but later leaves were unaffected by a soil bulk density of 1.5 g·cm-3. Net photosynthesis (Pn) and transpiration (E) increased linearly with increasing soil bulk density the first year, but the second year a nonlinear pattern was observed with highest rates at 1.3 and 1.4 g·cm-3. Soil bulk density of 1.5 g·cm-3 reduced number of leaves, leaf area and shoot length and advanced bloom 16 days on `Chambourcin' vines on six rootstocks with no interaction of rootstock and soil compaction. Withholding water for 8 days reduced Pn and E in all treatments, with no effect on shoot length, leaf, stem and total dry weights. Moisture stress in the noncompacted soil caused a reduction in leaf concentration of fructose, glucose and myo-inositol, but moisture stress had no effect in the compacted soil. Moisture stress caused a reduction in sucrose in both compacted and noncompacted soil. Compacting soil to a bulk density of 1.5 g·cm-3 was associated with an increase in leaf N, Ca, Mg, Al, Fe, Mn, Na, and Zn and a decrease in P, K, B, and Mo.

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Celia M. Cantín, Carlos H. Crisosto, and Kevin R. Day

Lauderdale, FL) and compared with bulk-packed fruit (control). On arrival at Kearney, the pallet was placed in cold storage (0 °C and 85% RH). Five boxes per treatment were removed from cold storage after 30, 45, and 60 d for quality attributes and shelf life

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Youping Sun, Ji Jhong Chen, Haifeng Xing, Asmita Paudel, Genhua Niu, and Matthew Chappell

), which did exhibit slight foliar damage. When irrigated with saline solution at an EC of 10.0 dS·m −1 , all V. × burkwoodii , V. dentatum ‘Christom’, V. dentatum var. deamii ‘SMVDLS’, V. בNCVX1’, V. nudum ‘Bulk’, and V. pragense ‘Decker

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K.G. Haynes and F.L. Haynes

A base population of high specific gravity clones was established from a diploid hybrid population of Solarium tuberosum Group Phureja and Solarium tuberosum Group Stenotomum previously adapted to the long-day growing conditions in North Carolina. This base population was subjected to two 2-year cycles of recurrent selection. During each cycle, selections in the field were made on the basis of tuber smoothness, shape, and size. Tubers from unselected clones were bulked by plots. Tuber specific gravity was determined for the selected and unselected (bulk) clones. Tuber specific gravity was significantly greater in the selected than in the unselected clones in each cycle of selection.

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Johann S. Buck and Michael R. Evans

million tons of fresh rice hulls were produced annually in the United States. According to Bunt (1988) and Hanan (1998) , fresh rice hulls had a bulk density of 0.10 g·cm −3 , water-holding capacity of 20% (v/v), total pore space of 89% (v/v), and an

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George Kotsiris, Panayiotis A. Nektarios, and Angeliki T. Paraskevopoulou

as well as the porosity and both the dry and saturated bulk density were determined for each substrate according to Nektarios et al. (2011b and 2011a , respectively). In situ substrate moisture content. The moisture content of the substrates was

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K. Ukoskit, P.G. Thompson, C.E. Watson Jr., and G.W. Lawrence

The inheritance of resistance to root-knot nematode race 3 [Meloidogyne incognita (Kofoid & White) Chitwood] in sweetpotato [Ipomoea batatas (L.) Lam.] was studied in 71 progenies of the F1 single-cross population produced from the cross of resistant parent `Regal' and susceptible parent `Vardaman'. The distribution frequency of the progenies based on log total nematode number (egg + juvenile counts) was a bimodal distribution with a ratio of ≈4 resistant : 1 susceptible. Based on this phenotypic ratio, the proposed genetic model was duplex polysomic inheritance (RRrrrr = resistant parent and rrrrrr = susceptible parent). Bulk segregant analysis in conjunction with the RAPD technique was used to identify a RAPD marker linked to a root-knot-nematode-resistance gene. Of 760 random decamer primers screened, 9 showed polymorphic bands between the two bulk DNA samples. Primer OPI51500 produced a band in the resistant bulk but not in the susceptible bulk, suggesting a linkage in coupling phase. An estimated recombination fraction of 0.2421 ± 0.057 between the marker and the root-knot-nematode-resistance gene indicated linkage.

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Thomas M. Contrisciano and E. Jay Holcomb

The objective of this research was to develop a mineral wool based growing medium for the horticultural industry. Two types of hydrophilic mineral wool, clean wool (CW) and unclean wool (UC) were used unamended, as well as both types in combinations with 25, 50, and 75 percent peat moss (PM). A control of 100 percent (PM) was also used. Unamended CW had a low bulk density, excellent water holding capacity, good aeration, but high pH. Once PM was added to CW, bulk density still remained low, water holding capacity and aeration remained good, and the pH dropped to a more suitable level. Unamended UW had a high bulk density, good water holding capacity, poor aeration, and high pH. Once PM was added to UW, bulk density decreased, water holding capacity remained good, aeration increased, and pH decreased to a more optimal level. Impatiens `Violet' and Begonia `Whiskey' were grown in the nine treatments for six and nine weeks respectively. At harvest, plant growth was evaluated by height, diameter, fresh weight, dry weight, and tissue analysis. Plant growth response showed plants grown in unamended CW, UW, and PM were smaller in size and lighter in fresh and dry weights than those in 50 percent wool/50 percent PM. The plants grown in 25 and 75 percent PM were similar to the 50 percent wool/50 percent PM in size and weight.

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Genet Teshome Mekuria, Graham Collins, Margaret Sedgley, and Shimon Lavee

Olive leaf spot is a disease of olive (Olea europaea L.) caused by the fungal pathogen, Spilocea oleaginea Cast. Progeny derived from crosses among susceptible, resistant, and semiresistant parental lines were assessed in the field for 8 years and classified as either resistant or susceptible. DNA from some of the progeny of this segregating population was used to identify molecular markers linked to olive leaf spot disease using the randomly amplified polymorphic DNA (RAPD) technique and bulked segregant analysis (BSA). Two DNA bulks were constructed, each containing 13 progeny showing either resistance or susceptibility for the disease, and screened for polymorphisms using 100 primers. One primer produced two polymorphic bands, one of ≈700 base pairs (bp) from the susceptible bulk and the other of ≈780 bp from the resistant bulk. The 780 bp marker appeared in 70.6% of the segregating progeny and 100% of parents showing resistance to leaf spot disease, while the 700 bp marker appeared in 47.1% of the segregating progeny and 100% of the parents showing susceptibility. These markers can be used as screening tools in olive improvement programs.