Search Results

You are looking at 81 - 90 of 780 items for :

  • All content x
Clear All
Full access

Li-Xiao Yao, Yong-Rui He, Hai-Fang Fan, Lan-Zhen Xu, Tian-Gang Lei, Xiu-Ping Zou, Ai-Hong Peng, Qiang Li, and Shan-Chun Chen

sequence of citrus was searched through BLASTx against the amino acid sequence of CjFRO1 . Five predicted sequences of C. sinensis (Cs3g01120, Cs9g19350, Cs5g06040, orange1.1t00399, and Cs9g19360) were found. Cs9g19350 corresponds to CjFRO1 , and the

Free access

Guihong Bi*, Carolyn Scagel, Lailiang Cheng, and Leslie Fuchigami

June-budded `Nonpareil/Nemaguard' almond (Prunus dulcis (Mill) D.A. Webb) trees were fertigated with one of five nitrogen (N) concentrations (0, 5, 10, 15, or 20 mm) in a modified Hoagland's solution from July to September. In October, the trees were sprayed twice with either water or 3% urea, then harvested after natural leaf fall and stored at 2°C. Trees were destructively sampled during winter storage to determine their concentrations of amino acids, protein, and non-structural carbohydrates (TNC). Increasing N supply either via N fertigation during the growing season or with foliar urea applications in the fall increased the concentrations of both free and total amino acids, whereas decreased their C/N ratios. Moreover, as the N supply increased, the proportion of nitrogen stored as free amino acids also increased. However, protein was still the main form of N used for storage. The predominant amino acid in both the free and total amino-acid pools was arginine. Arginin N accounted for an increasing proportion of the total N in both the free and total amino acids as the N supply was increased. However, the proportion of arginine N was higher in the free amino acids than in the total amino acids. A negative relationship was found between total amino acid and non-structural carbohydrate concentrations, suggesting that TNC is increasingly used for N assimilation as the supply of N increases. Urea applications decreased the concentrations of glucose, fructose, and sucrose, but had little influence on concentrations of sorbitol and starch. We conclude that protein is the primary form of storage N, and that arginine is the predominant amino acid. Furthermore, the synthesis of amino acids and proteins comes at the expense of non-structural carbohydrates.

Free access

Chenping Xu and Beiquan Mou

– 15.28A 665 , and C x (mg/L) = (1000A 470 – 1.63C a – 104.96C b )/221. Reducing power and contents of protein and amino acid. For analyzing nutritional value, leaf samples were soaked in liquid N immediately after harvest and stored at −80 °C. Leaf

Free access

Ed Stover, Richard R. Stange Jr., T. Gregory McCollum, Jesse Jaynes, Michael Irey, and Erik Mirkov

Methods Antimicrobial peptides. The AMPs listed in Table 1 include many reported in the literature as occurring naturally in diverse organisms and were custom-synthesized based on reported amino acid sequences: apidaecin, drosocin, histatin-5

Free access

Chenping Xu, Zhongchun Jiang, and Bingru Huang

., 1990 ). The metabolic processes and enzymes affected by N availability in immature leaves and mature leaves may vary. Nitrogen catabolism is predominant in mature or senescent leaves, in which proteins are degraded to produce NH 3 and amino acids

Free access

Tatsiana Espevig, Chenping Xu, Trygve S. Aamlid, Michelle DaCosta, and Bingru Huang

might be the result of amino acid sequence differences in the different isoforms. Alternatively, one gene product may undergo different co- and/or post-translational modifications that affect its pI and/or molecular weight. Table 2. Relative abundance of

Free access

Peter Nveawiah-Yoho, Jing Zhou, Marsha Palmer, Roger Sauve, Suping Zhou, Kevin J. Howe, Tara Fish, and Theodore W. Thannhauser

matching names were searched using amino acid sequences retrieved in the annotated tomato genome database ( Bombarely et al., 2011 ). For proteins assigned to multiple accessions in A. thaliana , the one annotated to salt or relevant stress factors and

Free access

Paweł Wójcik, Anna Skorupińska, and Hamide Gubbuk

-containing preparations (classified by the European Union as growth regulators) are also critical factors limiting their use in plant production. To overcome the problems related to use of auxins, application of the amino acid L-TRP, a precursor of IAA ( Pattison et al

Free access

Hai-nan Liu, Jian-rong Feng, Xiao-fang Liu, Wen-hui Li, Wen-juan Lv, and Ming Luo

sequencing ( Fig. 2B ). The deduced amino acid sequence of the fragment contained an F-box domain, V1, V2, HVa, and HVb. The structural characteristics were similar to SFB in other Rosaceae species. The deduced amino acid sequence had 91% identity with the

Free access

Robert A. Saftner

The ethylene precursor, 1 -aminocyclopropane- 1 -carboxylic acid (ACC), is actively transported across the tonoplast of plant cells, impacting cellular compartmentation of ACC and ethylene biosynthesis. To identify potential photoaffinity probes for identifying ACC transport-related membrane proteins, the effects of over 70 ACC and other amino acid analogs on ACC uptake into isolated maize vacuoles were investigated. Only relatively nonpolar, neutral amino acid stereoisomers of L-configuration were strong inhibitors of ACC transport. Group additions, substitutions, or deletions at the carboxyl, (x-amino and the Pro-(R) methylene, or hydrogen moieties essentially eliminated transport inhibition, whereas side-chain substitutions remained antagonistic. The kinetics of ACC and neutral L-amino acid analogs tested were competitive. The results indicate that the ACC transport system can be classified as a neutral L-amino acid carrier having a relatively high affinity for ACC and other nonpolar amino acids. The results also suggest that the carrier interacts with the carboxyl, alpha-amino, and Pro-(R) groups and the side chain of substrate amino acids. Based on these findings, potential photoaffinity probes of the ACC transport system have been identified.