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Yanbin Su, Yumei Liu, Huolin Shen, Xingguo Xiao, Zhansheng Li, Zhiyuan Fang, Limei Yang, Mu Zhuang, and Yangyong Zhang

45 s at 72 °C, and a final extension for 7 min at 72 °C. PCR amplifications were carried out in a GeneAmp PCR system 9700 (Applied Biosystems, Foster City, CA). The resulting products were subjected to 8% polyacrylamide gel electrophoresis at 160 V

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Yali He and Bingru Huang

. Electrophoresis for isozyme assays. Samples were subjected to discontinuous polyacrylamide gel electrophoresis under nondenaturing, nonreducing conditions as described by Laemmli (1970) with some modifications. SOD was detected on 10.8% acrylamide gels, and

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Sasmita Mishra, Scott Heckathorn, Jonathan Frantz, Futong Yu, and John Gray

by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), using 12% acrylamide, 16 × 20-cm gels (50 μg of total protein per lane) (e.g., Heckathorn et al., 2002 ). Following electrophoresis, gels were stained for total proteins using

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Patrick Burgess and Bingru Huang

by means of two-dimensional polyacrylamide gel electrophoresis separation and mass spectrometry (MS) identification has effectively described changes in proteomic abundance within various tissues of different plant species responding to abiotic

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Li-Juan Zhang, Tian-Xiu Zhong, Li-Xin Xu, Lie-bao Han, and Xunzhong Zhang

separated on nondenaturating native polyacrylamide gel electrophoresis. The procedure for protein extraction was the same as for TSP, and the protein was extracted from 0.2 g frozen leaves with 1 mL extraction buffer. Native PAGE was conducted using a mini

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Xunzhong Zhang, Erik H. Ervin, Yiming Liu, Guofu Hu, Chao Shang, Takeshi Fukao, and Jasper Alpuerto

antioxidant enzymes. The extracts (15 µL) for SOD, CAT, and APX were loaded on each gel. Native polyacrylamide gel electrophoresis (PAGE) was performed using a Mini-Protean system (Bio-Rad Laboratories, Hercules, CA) at 4 °C, 120 V for 90 min, except that SDS

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Zhuang-Zhuang Liu, Tao Chen, Fang-Ren Peng, You-Wang Liang, Peng-Peng Tan, Zheng-Hai Mo, Fan Cao, Yang-Juan Shang, Rui Zhang, and Yong-Rong Li

poor safety, low efficiency, and low resolution of the MSAP technique involving polyacrylamide gel electrophoresis, Xu et al. (2005) introduced a fluorescence labeling system with capillary electrophoresis for MSAP, named F-MSAP, which is safe for

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Qianqian Shi, Xiaoxiao Zhang, Xiang Li, Lijuan Zhai, Xiaoning Luo, Jianrang Luo, Lixia He, Yanlong Zhang, and Long Li

, CA) at Biomarker Technologies (Beijing, China). First, sRNAs (18–30 nt) were isolated from the total RNA by 15% TBE–urea denaturing polyacrylamide gels electrophoresis, and a 5′ RNA adaptor (GTTCAGAGTTCTACAGTCCGACGATC) and 3′ RNA adaptor

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Xiaohe Song and Zhanao Deng

genomic DNA from each progeny of the respective bulks was combined. The two DNA bulks and the genomic DNA of UFGE 4033 and ‘Sunburst Snow White’ were run simultaneously in polymerase chain reaction (PCR) amplification and polyacrylamide gel electrophoresis

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Jing Tian, Li-Ping Wang, Yan-Juan Yang, Jin Sun, and Shi-Rong Guo

,000 g n for 20 min at 4 °C, and the supernatant was centrifuged again. For the extraction of APX, 10 m m AsA and 1 m m EDTA were added. Native polyacrylamide gel electrophoresis (PAGE), using 3% concentrated gel and 8% separating gel, was performed at