Search Results

You are looking at 71 - 80 of 273 items for :

  • real-time PCR x
  • All content x
Clear All
Full access

Mokhles A. Elsysy and Peter M. Hirst

( Untergasser et al., 2012 ) ( Table 1 ). Real-time PCR was then carried out on a CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA), with PerfeCTa SYBR Green FastMix (Quantabio, Beverly, MA) in a 15-μL final volume to determine the

Free access

Cecilia E. McGregor, Douglas W. Miano, Don R. LaBonte, Mary Hoy, Chris A. Clark, and Guilherme J.M. Rosa

and quantitative real-time polymerase chain reaction (Q-RT-PCR) for virus titer determination. Leaf material was ground to a fine powder in liquid nitrogen with a mortar and pestle and ≈50 mg was used to extract total RNA using the RNeasy Mini Kit

Free access

Oleg Daugovish, Mark Bolda, Sukhwinder Kaur, Maren J. Mochizuki, Daniel Marcum, and Lynn Epstein

cycles of 95 °C for 15 s and 60 °C for 1 min. PCR was performed in a 7500 Fast Real-Time PCR System (Applied Biosystems). The sample cycle threshold values in comparison with the standard were used to calculate the quantity of C. acutatum DNA. The

Free access

Madhurababu Kunta, John V. da Graça, Nasir S.A. Malik, Eliezer S. Louzada, and Mamoudou Sétamou

. da Graça, J.V. 2012 First report of citrus Huanglongbing in Texas Phytopathology 102 S4.66 Li, W. Hartung, J.S. Levy, L. 2006 Quantitative real-time PCR for detection and identification of Candidatus Liberibacter species associated with citrus

Free access

Jollanda Effendy, Don R. La Bonte, and Niranjan Baisakh

(Bio-Rad), 2 μL of cDNA, and 3.25 pmol DEG-specific primers ( Table 1 ) in a MyiQ real-time PCR analyses system (Bio-Rad). The relative expression ratio was calculated using the 2- ΔΔCt method ( Ramanarao et al., 2011 ) with sweetpotato elongation

Free access

Xue Li, Chen Zang, Hang Ge, Jing Zhang, Donald Grierson, Xue-ren Yin, and Kun-song Chen

template of real-time polymerase chain reaction (PCR). Real-time PCR. Oligonucleotide primers for real-time PCR were designed from the untranslated region of individual genes and are listed in Table 2 . Loquat actin was used as an internal standard ( Shan

Free access

Ji Tian, Ke-ting Li, Shi-ya Zhang, Jie Zhang, Ting-ting Song, Yong-jun Zhu, and Yun-cong Yao

real-time PCR analysis. Total RNA was extracted from crabapple leaves using an RNA Extraction Kit (Aidlab, Beijing, China) according to the manufacturer’s instructions. DNase I (TaKaRa, Ohtsu, Japan) was added to remove genomic DNA, and the samples were

Free access

Meiling Zhang, Ming Chen, Zhen Wang, Ting Wu, Yi Wang, Xinzhong Zhang, and Zhenhai Han

quantitative real-time polymerase chain reaction (PCR). Total RNA of root samples was extracted by Cetyltrimethylammonium bromide (CTAB; Sinopharm Chemical Reagent Co., Ltd., Beijing, China) method ( Zhang et al., 2005 ). The first-strand cDNA was synthesized

Free access

Kai Zhao, Feng Zhang, Yi Yang, Yue Ma, Yuexue Liu, He Li, Hongyan Dai, and Zhihong Zhang

densities ( Atkinson and Else, 2001 ), and allow mechanical harvesting. Development of compact apples through conventional breeding may control growth ( Talwara et al., 2013 ), but it is a costly and time-consuming approach with a high probability of adverse

Full access

Yu Bai, Ying Zhou, Xiaoqing Tang, Yu Wang, Fangquan Wang, and Jie Yang

candidate genes related to the flowering pathway were analyzed by quantitative real time polymerase chain reaction (qRT-PCR). The results of this study will enhance our understanding of the bolting and flowering-time regulatory networks in I. indigotica