reverse primer pair sequences, simple sequence repeat (SSR) type, annealing temperature (Tm), and length of the 28 polymorphic expressed sequence tag–SSR markers that were used for bulked segregant analysis in Primula forbesii . The percentage of the
Cunquan Yuan, Zhiyi Qu, Huitang Pan, Tangren Cheng, Jia Wang, and Qixiang Zhang
et al., 2001 ), increase SOM, decrease erosion by water and wind ( Tyler, 2001 ), and reduce nutrient leaching ( Jaber et al., 2005 ; Yang et al., 2007 ). Increased SOM improves physical properties by decreasing bulk density and increasing available
James E. Altland, James S. Owen Jr., Brian E. Jackson, and Jeb S. Fields
linear decrease in AS with a concomitant increase in CC and no change in TP. Bulk density increased from 0.15 to 0.17 g·cm −3 ( P = 0.1039) with variation by source within each age group ( P = 0.0147). This agrees with Bilderback et al. (2005) , who
Tripti Vashisth and Taylor Livingston
significant root loss, and the C Las presence can be detected in the root system even before visible symptoms appear on the tree, suggesting that C Las first colonizes in roots due to bulk phloem flow. Once infected by C Las, plugging in phloem sieve pores
Kirk W. Pomper, Anita N. Azarenko, Joel W. Davis, and Shawn A. Mehlenbacher
Random amplified polymorphic DNA (RAPD) markers were identified for self-incompatibility (SI) alleles that will allow marker-assisted selection of desired S-alleles and assist in cloning the locus responsible for the sporophytic SI displayed in hazelnut (Corylus avellana L.). DNA was extracted from young leaves collected from field-planted parents and 27 progeny of the cross OSU 23.017 (S1 S12) × VR6-28 (S2 S26). Screening of 10-base oligonucleotide RAPD primers was performed using bulked segregant analysis. DNA samples from six trees each were pooled into four “bulks,” one for each of the following: S1 S2, S1 S26, S2 S12, and S12 S26. “Super bulks” of twelve trees each for S1, S2, S12, and S26 then were created for each allele by combining the appropriate bulks. The DNA from these four super bulks and also the parents was used as a template in the PCR assays. Amplification products were electrophoresed on 2% agarose gels and photographed under UV light after ethidium bromide staining. 200 primers were screened and one RAPD marker each was identified for alleles S2 (OPI-07700) and S1 (OPJ-141700).
Rebecca J. Long, Rebecca N. Brown, and José A. Amador
sludge as well as repeated application of MSW compost and biosolids or BS have been shown to increase soil C and OM levels and lower bulk density ( Douglas et al., 2003 ; Foley and Cooperband, 2002 ; Gagnon et al., 2001 ; Hargreaves et al., 2008
Paolo Sambo, Franco Sannazzaro, and Michael R. Evans
, available water content, easily available water, and bulk density are typically the physical properties of greatest interest for substrates to be used in greenhouse crop production. Peat is one of the most common components used in the formulation of root
Kittipat Ukoskit, Paul G. Thompson, Gary W. Lawrence, and Clarence E. Watson
The inheritance of root-knot nematode race 3 [Meloidogyne incognita (Kofoid & White) Chitwood] resistance was studied in 71 progenies of the F1 backcross population produced from the resistant parent `Regal' and the susceptible parent `Vardaman'. The distribution frequency of the progenies measured on total nematode number (eggs + juveniles) indicated a bimodal distribution with a ratio of 4 resistant: 1 susceptible. Based on this phenotypic ratio, the proposed genetic model was duplex polysomic inheritance (RRrrrr = resistant). Bulk segregant analysis in conjunction with the RAPD technique was employed to identify RAPD marker linked to the root knot nematode-resistant gene. Nine of 760 random decamer primers screened showed polymorphic bands. Primer OPI51500 produced a band in the resistant bulk, but not in the susceptible bulk. Estimated recombination frequency of 0.24 between the OPI51500 marker and the root-knot nematode-resistant gene indicated linkage.
Tyler C. Hoskins, James S. Owen Jr., Jeb S. Fields, James E. Altland, Zachary M. Easton, and Alex X. Niemiera
bark/sand blend used in this study], low bulk density (D b ), low ion exchange capacity, and low surface area. Because of these differences between soils and soilless substrates, an independent body of research on solute transport through pine bark
Soon O. Park* and Kevin M. Crosby
Mature fruit size and shape are important traits of most melon types. Our objective was to identify RAPD markers associated with major QTL affecting fruit weight, length, diameter and shape by means of bulked segregant analysis in an F2 population from the ananas melon cross of Deltex (larger fruit size) × TGR1551 (smaller fruit size). Clear separations for fruit weight, length, diameter, and shape between Deltex and TGR1551 were observed. Continuous distributions for fruit weight, length, diameter and shape were found in the F2 population indicating quantitative inheritance for the fruit traits. Significant positive correlations were detected between fruit weight and shape traits (r = 0.73 to 0.80). A significant positive correlation was observed between fruit weight and glucose (r = 0.35) or fructose (r = 0.25), whereas no correlation was noted between fruit weight and sucrose or total soluble solids. Two small and large bulks for fruit weight and shape were developed from F2 plants. A total of 240 primers were used to simultaneously screen between the small and large bulks, and between Deltex and TGR1551. Twenty-six RAPD markers were polymorphic for the small and large bulks. Ten markers were found to be significantly and consistently associated with fruit size and shape traits on the basis of simple linear regression. Of the 10 markers associated, four displayed an amplified DNA fragment in the small bulk, while six showed an amplified DNA fragment in the large bulk. The associated marker OJ07.350 explained 15% to 27% of the phenotypic variation for the fruit traits. These markers associated with QTL for melon fruit size and shape are expected to be useful in melon breeding programs for modifying fruit size.