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Yong In Kuk and Ji San Shin

leaf age. Native polyacrylamide gel electrophoresis and activity staining. The isozymes of APX were separated on nondenaturating polyacrylamide gels, according to methods described by Laemmli (1970) , with only slight modifications. Equal

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Choun-Sea Lin, Nien-Tzu Liu, De-Chih Liao, Jau-Song Yu, Chuang-Hwei Tsao, Chao-Hsiung Lin, Chih-Wen Sun, Wann-Neng Jane, Hsing Sheng Tsay, Jeremy Jian-Wei Chen, Erh-Min Lai, Na-Sheng Lin, Wei-Chin Chang, and Chung-Chih Lin

gel electrophoresis (SDS-PAGE) system (Mini-P III; Bio-Rad), and covered by 1% agarose containing bromphenol blue. The SDS-PAGE was performed at 100 V for 120 min, following which the gel was stained by Coomassie blue. The experiments were repeated

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Alexis K. Nagel, Guido Schnabel, Cesar Petri, and Ralph Scorza

gel electrophoresis was performed on total cellular protein using a Mini-Protean ® 3 with 18% Tris-HCl Ready Gels (Bio-Rad Laboratories, Hercules, CA). Proteins were transferred to an immunoblot polyvinyl difluoride membrane using a Mini

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Mason T. MacDonald, Rajasekaran R. Lada, A. Robin Robinson, and Jeff Hoyle

, B.D. 1990 One-dimensional polyacrylamide gel electrophoresis 1 91 Hames B.D. Richwood D. Gel electrophoresis of protein: A practical approach Oxford University Press New York, NY Howitt, C

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Haiying Zhang, Jianguang Fan, Shaogui Guo, Yi Ren, Guoyi Gong, Jie Zhang, Yiqun Weng, Angela Davis, and Yong Xu

s, 55 °C for 20 s, and 72 °C for 90 s with a final extension at 72 °C for 8 min. The PCR products were analyzed using 6% polyacrylamide gel electrophoresis in 1× Tris borate ethylenediaminetetraacetic acid buffer. The gel was stained with silver

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Metka Sisko, Branka Javornik, Aleksander Siftar, and Anton Ivancic

polyacrylamide gel electrophoresis – based analysis of commercially important North American cultivars HortScience 41 304 309 Gianfranceschi, L. Seglias, N. Tarchini, R. Komjanc, M. Gessler, C. 1998

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Mark K. Ehlenfeldt and James J. Polashock

) . Polymerase chain reactions, polyacrylamide gel electrophoresis, and silver staining were as described ( Novy et al., 1994 ). Reliable polymorphic bands were scored as 1 (present) or 0 (absent). Cluster analysis and tree generation using NTSYS-pc (Exeter

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Matthew A. Escobar, Andrew Shilling, Pine Higgins, Sandra L. Uratsu, and Abhaya M. Dandekar

tissue, but activity was absent within the shell in pellicle and kernel tissues. As a complement to spectrophotometric PPO activity assays, protein extracts were separated using native polyacrylamide gel electrophoresis and subjected to an in-gel PPO

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Xunzhong Zhang, Kehua Wang, and Erik H. Ervin

mixed with 100 μL of 2 × SDS buffer [0.125 mol·L −1 Tris/HCl, pH 6.8, 20% (v/v) glycerol, 0.01% (v/v) bromphenol blue, 200 m m dithiothreitol, and 4% (w/v) SDS] was used for Sodium dodeclysulfate polyacrylamide gel electrophoresis analysis ( Laemmli

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Rui Sun, Hui Li, Qiong Zhang, Dongmei Chen, Fengqiu Yang, Yongbo Zhao, Yi Wang, Yuepeng Han, Xinzhong Zhang, and Zhenhai Han

for 1 min; final extension at 72 °C for 5 min; and holding at 4 °C. The PCR products were separated using 8% polyacrylamide gel electrophoresis and then visualized by silver staining. The DNA ladder was “puc18/Msp I” (RealTimer BioTech Co., Beijing