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Tilin Fang, Yanqi Wu, Shiva Makaju, Todd Tribble, Dennis L. Martin, and Justin Q. Moss

experimental bermudagrass lines developed by the OSU turf bermudagrass breeding program were entered in the 2013 NTEP bermudagrass trial to allow for testing over a wide geographic area. DNA fingerprints to identify these bermudagrass entries have not been

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Qingguo Ma, Junpei Zhang, and Dong Pei

-efficiency, repeatability, and resolution compared with many other techniques ( Aggarwal et al., 2002 ). Therefore, AFLP markers have emerged as a major genetic marker with broad application in systematics, population genetics, DNA fingerprinting, and mapping quantitative

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Shanshan Cao, Stephen Stringer, Gunawati Gunawan, Cecilia McGregor, and Patrick J. Conner

fingerprinting ( Nybom et al., 2014 ; Semagn et al., 2006 ). The high level of polymorphism at each marker even among full siblings makes SSRs very useful for identifying parent–progeny relationships ( Bassil et al., 2012 ). SSR markers have been used to

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Michael J. Havey and Farhad Ghavami

onion populations, mapping of important phenotypes, fingerprinting of inbred lines and hybrids, and quality control of seed lots. Table 3. Genetic map positions of single nucleotide polymorphisms (SNPs) and mean allelic frequencies across 14 onion

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Amnon Levi and Claude E. Thomas

cultivars was assembled. The polymorphic markers can be useful in DNA fingerprinting of watermelon elite breeding lines, in differentiating among closely related diploid and tetraploid breeding lines, and in confirming the production of true F 1 hybrid

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C.E. Greer, R.E. Schutzki, A. Fernandez, and J.F. Hancock

Starch gel electrophoresis was used to fingerprint 55 Taxus plants, listed as 21 species and/or cultivars. Plants were analyzed for six enzymes, representing eight putative loci. Within many of the cultivars, different fingerprints were observed, indicating nomenclatural errors in Taxus.

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Barbara Gilmore, Nahla Bassil, April Nyberg, Brian Knaus, Don Smith, Danny L. Barney, and Kim Hummer

( Li et al., 2011 ; Sun et al., 2011 ). The objectives of this study were to develop new SSR markers using barcoded multiplexed libraries of multiple peony species, and to evaluate these markers for fingerprinting herbaceous peony ( P. lactiflora and

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R.N. Trigiano, M.C. Scott, and G. Caetano-Anollés

Four chrysanthemum (Dendranthema grandiflora) spontaneous and radiation-induced sports from the cultivar `Charm' and phenotypically differing only in flower color were individually characterized using arbitrary signatures from amplification profiles (ASAP). ASAP analysis is based on a two-step arbitrary primer amplification procedure that produces “fingerprints of fingerprints.” In the first step, `Charm', `Dark Charm', `Dark Bronze Charm', `Salmon Charm', and `Coral Charm' were fingerprinted by DNA amplification fingerprinting (DAF) with standard octamer arbitrary primers. Diluted products from three monomorphic fingerprints for each cultivar were subsequently reamplified using four minihairpin decamer primers. Each of the 12 ASAP profiles revealed about 30% polymorphic loci and some were used to uniquely identify cultivars and estimate genetic relationships. The ASAP technique permits identification of previously genetically indistinguishable plant material and should facilitate marker assisted breeding and protection of ownership rights.

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Todd A. Burnes, Robert A. Blanchette, Jason A. Smith, and James J. Luby

in labeling or propagation may have occurred and it was wrongly designated as immune. Recently, fingerprinting techniques using random amplified polymorphic DNA and intersimple sequence repeat markers have become available to determine relatedness of

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Maomao Ding, Ke Wang, Wenting Wang, Miaojin Chen, Dajun Wu, Changjie Xu, and Kunsong Chen

as maternal parent and ‘Hujingmilu’ as paternal one, in subgroup II-B. Clustering of progenies with either parent was observed ( Fig. 2 ). The authentication of hybrids was supported by the fingerprint profiles where the SSR bands of progenies were