:10 with MeOH and subjected to HPLC on a Kromasil C 18 RP column (250 × 4.6 mm, 5 μm, 0.7 mL·min −1 flow rate) with gradient elution using 40% to 100% aqueous MeOH and ultraviolet-Vis detection at 229 nm. An external standard curve method was used to
Philipp von Bieberstein, Ya-ming Xu, A.A. Leslie Gunatilaka, and Raphael Gruener
Samson Zeray Tesfay, Sakhile Mathe, Albert T. Modi, and Tafadzwanashe Mabhaudhi
radical scavenging ability of the extract was expressed as μmol Trolox equivalents per gram of plant material on dry basis. Nonstructural soluble CHOs. Sugars were determined using HPLC-refractive-index detector (RID) according to Liu et al. (1999) , with
Ying Wang, Tingting Xue, Xing Han, Lingxiao Guan, Liang Zhang, Hua Wang, and Hua Li
Technologies, Santa Clara, CA). All measurements were determined three times, and all parameters were analyzed in triplicate. HPLC analysis of monomeric anthocyanins in grape skins. Only monomeric anthocyanins of fully mature grapes (harvested 24 Aug. 2018
Tuan Anh Le, Zoltán Pék, Sándor Takács, András Neményi, Hussein G. Daood, and Lajos Helyes
-homogenized tomato by crushing in a crucible mortar and shaking for 15 min with 3% metaphosphoric acid solution. The mixture was filtered through a filter paper and purified by a 45-μm nylon syringe filter before injection on to the HPLC column. A Chromaster liquid
Dean A. Kopsell, Kimberly J. Whitlock, Carl E. Sams, and David E. Kopsell
Technologies, Santa Clara, CA) before HPLC analysis. Purslane shoot tissue pigment HPLC analysis. An HPLC unit with a photodiode array detector (1200 series; Agilent Technologies) was used for purslane pigment separation. Chromatographic separations were
william M. Walter Jr.
The sugar content of the sweetpotato cultivars Centennial, Coroner, Georgia Red, Jewel, and Sweet Red was measured by nign performance liquid chromatography (HPLC) and compared to the sugar content found by measuring the refractive index of cellular sap and converting the refractive index value to sugar concentration. The sugar content and refractive index values were measured for just-harvested, cured and stored roots. Changes in the sugar content as determined by refractive index were found to be linearly related to changes 1n sugar content measured by HPLC, indicating that this method can be used to monitor changes in postharvest sugar content.
W.Y.L. Poon and I.L. Goldman
1 Former graduate assistant. We thank Peter Vanderveer for assisrtance with HPLC analyses, Marilyn H.Y. Hovius for assistance with figures, and W.F. Tracy and T. Sharkey for helpful comments.
John A. McCallum, Meeghan Pither-Joyce, and Martin Shaw
This research was funded by the New Zealand Foundation for Research, Science and Technology. We thank Ruth Butler for statistical assistance, Lyall Simmons for HPLC analysis, and Jane Lancaster (AgriFood Solutions) for critical reading of this
V.I. Shattuck, Y. Kakuda, B.J. Shelp, and N. Kakuda
Abbreviations: GS, glucosinolate; HPLC, high-performance liquid chromatography. 1 Dept. of Horticultural Science. 2 Dept. of Food Science. We gratefully acknowledge E.W. Underhill for performing the LC plasma spray mass spectrometry analysis and
Joshua K. Craver, Joshua R. Gerovac, Roberto G. Lopez, and Dean A. Kopsell
Econofilter PTFE 25/20; Agilent Technologies, Wilmington, DE) using a 5-mL syringe (Becton, Dickinson and Co., Franklin Lakes, NJ) before HPLC analysis. Carotenoid and chlorophyll HPLC analysis. An HPLC unit with a photodiode array detector (1200 series