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Madhulika Sagaram and Jacqueline K. Burns

's instructions (Wizard genomic DNA purification kit; Promega, Madison, WI). Following extraction, samples were tested for the presence of the greening bacterium using real-time PCR analysis as described ( Satyanarayana et al., 2008 ). Chlorophyll

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Ben-Hong Wu, Shao-Hua Li, Marta Nosarzewski, and Douglas D. Archbold

branch shading and bending ( Ito et al., 2005 ). To understand how sorbitol metabolism is regulated, it is essential to have a comparative profile of SDH expression to a diverse set of conditions. Using real-time polymerase chain reaction (PCR), the

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Craig A. Ledbetter and Elizabeth E. Rogers

single DNA extraction was prepared from each petiole pair, and triplicate PCRs were performed. Xf quantification. Xf titer in petioles was measured by quantitative real-time PCR. Total DNA was extracted from petioles by an adaptation of a

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Ed Etxeberria, Pedro Gonzalez, Ariel Singerman, and Timothy Ebert

, V.G. Postnikova, E. Hartung, J.S. Stone, A.L. Damsteeg, V.D. Schneider, W.L. Munyaneza, J.E. Brlansky, R.H. 2013 Development of primers and probes for genus and species specific detection of ‘Candidatus Liberibacter Species’ by Real-Time PCR Plant

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Megan J. Bowman, David K. Willis, and Philipp W. Simon

“Minimum Information for Publication Quantitative Real-time PCR Experiments” or MIQE guidelines ( Bustin et al., 2009 ). These guidelines focus on extraction protocols, transcript quality, reference gene accuracy, and publication standards necessary for

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Abigail J. Walter, YongPing Duan, and David G. Hall

. Biol. 155 29 36 Li, W. Hartung, J.S. Levy, L. 2006 Quantitative real-time PCR for detection and identification of Candidatus Liberibacter species associated with citrus huanglongbing J. Microbiol. Methods 66 104 115 Morgan, J.K. Zhou, L. Li, W

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Yiran Li, Akiha Abe, Takuichi Fuse, Tomonari Hirano, Yoichiro Hoshino, and Hisato Kunitake

listed in Table 1 . Citrus constitutively expressed the actin gene of ‘Banpeiyu’ (accession no. GU911361) and ‘Hyuganatsu’ (accession no. XM_006432422) as an internal control. Real-time qRT-PCR was performed by a CFX manager real-time PCR detection

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Hong Zhu, Eric P. Beers, and Rongcai Yuan

and Yuan, 2008 ). One microgram of total RNA was used to synthesize cDNA in a 20 μL reaction volume using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA). Real-time quantitative polymerase chain reaction (PCR) was

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Rongcai Yuan and Jianguo Li

synthesize cDNA in a 20-μL reaction volume using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA). Real-time quantitative polymerase chain reaction (PCR) was performed using the Power SYBR Green PCR Master Mix Kit (Applied

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Mary Helen Ferguson, Christopher A. Clark, and Barbara J. Smith

thermic Typic Fragiudult ( USDA-NRCS, 2014 , 2016 ). In an initial effort to determine which study plants were infected, real-time polymerase chain reaction (PCR) was used to test stem sap for X. fastidiosa . One cane was cut from the base of each plant