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result of the high resolution ratio and repeatability, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is being widely used in molecular marker research for many different crops. However, SDS-PAGE cannot reveal much genetic

were made according to Milla et al. (2005) . After amplification, AFLP fragments were denatured at 94 °C and separated by polyacrylamide gel electrophoresis on a LI-COR 4300 DNA Analyzer Sequencer (LI-COR Biosciences, Lincoln, NE) on 25-cm 8% v / v

annealing temperature because of the different primers used. The amplification products were separated using 8% polyacrylamide gel electrophoresis at 75 W (Bio-Rad Electrophoresis system, Hercules, CA) for 2 h and then visualized using a simplified silver

/volume) agarose gel containing ethidium bromide 1.0 μg·mL –1 in 1 × TAE (40 m m Tris-acetate, 1 m m EDTA) buffer, observed under ultraviolet light and photographed. SSR PCR products were separated by polyacrylamide gel electrophoresis (6% gel) and silver

gel electrophoresis was performed on total cellular protein using a Mini-Protean ® 3 with 18% Tris-HCl Ready Gels (Bio-Rad Laboratories, Hercules, CA). Proteins were transferred to an immunoblot polyvinyl difluoride membrane using a Mini

Mol. Biol. Rpt. 13 207 209 Ghosh, A.K. Lukens, L.N. Hunter, D.M. Strommer, J.N. 2006 European and asian pears: Simple sequence repeat-polyacrylamide gel electrophoresis-based analysis of

antioxidant enzyme. The extracts (15 μL) for SOD, CAT, and POD were loaded on each gel. Native polyacrylamide gel electrophoresis (PAGE) was performed using a Mini-Protean system (Bio-Rad Laboratories, Hercules, CA) at 4 °C, 120 V for 90 min, except that

3–10], and the concentration was determined according to Bradford (1976) using bovine serum albumin as standard. Protein separation and image analysis. Proteins were separated by two-dimensional polyacrylamide gel electrophoresis (2D

volume of 1 mL, as described by Fernández-García et al. (2004) . The mean value of at least three replications was taken as indicator of peroxidase activity. Determination of isoperoxidase profile by native polyacrylamide gel electrophoresis (PAGE

) . Polymerase chain reactions, polyacrylamide gel electrophoresis, and silver staining were as described ( Novy et al., 1994 ). Reliable polymorphic bands were scored as 1 (present) or 0 (absent). Cluster analysis and tree generation using NTSYS-pc (Exeter