result of the high resolution ratio and repeatability, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is being widely used in molecular marker research for many different crops. However, SDS-PAGE cannot reveal much genetic
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Yuan Zhang, Chen Wang, HongZheng Ma, and SiLan Dai
Jennifer A. Kimball, M. Carolina Zuleta, Matthew C. Martin, Kevin E. Kenworthy, Ambika Chandra, and Susana R. Milla-Lewis
were made according to Milla et al. (2005) . After amplification, AFLP fragments were denatured at 94 °C and separated by polyacrylamide gel electrophoresis on a LI-COR 4300 DNA Analyzer Sequencer (LI-COR Biosciences, Lincoln, NE) on 25-cm 8% v / v
Xiu Cai Fan, Hai Sheng Sun, Ying Zhang, Jian Fu Jiang, Min Li, and Chong Huai Liu
annealing temperature because of the different primers used. The amplification products were separated using 8% polyacrylamide gel electrophoresis at 75 W (Bio-Rad Electrophoresis system, Hercules, CA) for 2 h and then visualized using a simplified silver
Aoxue Wang, Fanjuan Meng, Xiangyang Xu, Yong Wang, and Jingfu Li
/volume) agarose gel containing ethidium bromide 1.0 μg·mL –1 in 1 × TAE (40 m m Tris-acetate, 1 m m EDTA) buffer, observed under ultraviolet light and photographed. SSR PCR products were separated by polyacrylamide gel electrophoresis (6% gel) and silver
Alexis K. Nagel, Guido Schnabel, Cesar Petri, and Ralph Scorza
gel electrophoresis was performed on total cellular protein using a Mini-Protean ® 3 with 18% Tris-HCl Ready Gels (Bio-Rad Laboratories, Hercules, CA). Proteins were transferred to an immunoblot polyvinyl difluoride membrane using a Mini
Carlos Miranda, Jorge Urrestarazu, Luis G. Santesteban, José B. Royo, and Valero Urbina
Mol. Biol. Rpt. 13 207 209 Ghosh, A.K. Lukens, L.N. Hunter, D.M. Strommer, J.N. 2006 European and asian pears: Simple sequence repeat-polyacrylamide gel electrophoresis-based analysis of
Feifei Li, Da Zhan, Lixin Xu, Liebao Han, and Xunzhong Zhang
antioxidant enzyme. The extracts (15 μL) for SOD, CAT, and POD were loaded on each gel. Native polyacrylamide gel electrophoresis (PAGE) was performed using a Mini-Protean system (Bio-Rad Laboratories, Hercules, CA) at 4 °C, 120 V for 90 min, except that
Misael O. Vega-García, Greici López-Espinoza, Jeanett Chávez Ontiveros, José J. Caro-Corrales, Francisco Delgado Vargas, and José A. López-Valenzuela
3–10], and the concentration was determined according to Bradford (1976) using bovine serum albumin as standard. Protein separation and image analysis. Proteins were separated by two-dimensional polyacrylamide gel electrophoresis (2D
Olfa Zarrouk, Pilar S. Testillano, María Carmen Risueño, María Ángeles Moreno, and Yolanda Gogorcena
volume of 1 mL, as described by Fernández-García et al. (2004) . The mean value of at least three replications was taken as indicator of peroxidase activity. Determination of isoperoxidase profile by native polyacrylamide gel electrophoresis (PAGE
Mark K. Ehlenfeldt and James J. Polashock
) . Polymerase chain reactions, polyacrylamide gel electrophoresis, and silver staining were as described ( Novy et al., 1994 ). Reliable polymorphic bands were scored as 1 (present) or 0 (absent). Cluster analysis and tree generation using NTSYS-pc (Exeter