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Shigenori Yaguchi, Tetsuya Nakajima, Toshihisa Sumi, Naoki Yamauchi, and Masayoshi Shigyo

of the second PCR products was incubated for 2 h at 37 °C in a volume of 15 μL using 2 U of a restriction enzyme and subsequently resolved by 5% denaturing polyacrylamide gel electrophoresis (PAGE) with silver staining according to the procedure of

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Yuan Zhang, Chen Wang, HongZheng Ma, and SiLan Dai

result of the high resolution ratio and repeatability, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is being widely used in molecular marker research for many different crops. However, SDS-PAGE cannot reveal much genetic

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Xiu Cai Fan, Hai Sheng Sun, Ying Zhang, Jian Fu Jiang, Min Li, and Chong Huai Liu

annealing temperature because of the different primers used. The amplification products were separated using 8% polyacrylamide gel electrophoresis at 75 W (Bio-Rad Electrophoresis system, Hercules, CA) for 2 h and then visualized using a simplified silver

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Aoxue Wang, Fanjuan Meng, Xiangyang Xu, Yong Wang, and Jingfu Li

/volume) agarose gel containing ethidium bromide 1.0 μg·mL –1 in 1 × TAE (40 m m Tris-acetate, 1 m m EDTA) buffer, observed under ultraviolet light and photographed. SSR PCR products were separated by polyacrylamide gel electrophoresis (6% gel) and silver

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Feifei Li, Da Zhan, Lixin Xu, Liebao Han, and Xunzhong Zhang

antioxidant enzyme. The extracts (15 μL) for SOD, CAT, and POD were loaded on each gel. Native polyacrylamide gel electrophoresis (PAGE) was performed using a Mini-Protean system (Bio-Rad Laboratories, Hercules, CA) at 4 °C, 120 V for 90 min, except that

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Huiling Wang, Wei Wang, Weidong Huang, and Haiying Xu

/v) polyvinylpyrrolidone. Protein concentration was determined as described in Bardford (1976) using bovine serum albumin as the standard. The separation of total proteins was performed using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in 12

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Yanbin Su, Yumei Liu, Huolin Shen, Xingguo Xiao, Zhansheng Li, Zhiyuan Fang, Limei Yang, Mu Zhuang, and Yangyong Zhang

45 s at 72 °C, and a final extension for 7 min at 72 °C. PCR amplifications were carried out in a GeneAmp PCR system 9700 (Applied Biosystems, Foster City, CA). The resulting products were subjected to 8% polyacrylamide gel electrophoresis at 160 V

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Misael O. Vega-García, Greici López-Espinoza, Jeanett Chávez Ontiveros, José J. Caro-Corrales, Francisco Delgado Vargas, and José A. López-Valenzuela

3–10], and the concentration was determined according to Bradford (1976) using bovine serum albumin as standard. Protein separation and image analysis. Proteins were separated by two-dimensional polyacrylamide gel electrophoresis (2D

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Olfa Zarrouk, Pilar S. Testillano, María Carmen Risueño, María Ángeles Moreno, and Yolanda Gogorcena

volume of 1 mL, as described by Fernández-García et al. (2004) . The mean value of at least three replications was taken as indicator of peroxidase activity. Determination of isoperoxidase profile by native polyacrylamide gel electrophoresis (PAGE

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Carlos Miranda, Jorge Urrestarazu, Luis G. Santesteban, José B. Royo, and Valero Urbina

Mol. Biol. Rpt. 13 207 209 Ghosh, A.K. Lukens, L.N. Hunter, D.M. Strommer, J.N. 2006 European and asian pears: Simple sequence repeat-polyacrylamide gel electrophoresis-based analysis of