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Tilin Fang, Yanqi Wu, Shiva Makaju, Todd Tribble, Dennis L. Martin, and Justin Q. Moss

established. Identification of bermudagrass varieties was challenging until DNA molecular markers were developed, providing powerful identification tools over the last several years. For example, using 11 SSR markers, 32 commercially released clonal varieties

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Karen R. Harris-Shultz, Brian M. Schwartz, and Jeff A. Brady

( Harris-Shultz et al., 2010 ). This creates problems in the protection of cultivar proprietary rights and plant stock certification. The objectives of this study were to identify SSR markers that can distinguish between genotypes in the ‘Tifgreen’ family

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David Jesús Gil-Ariza, Iraida Amaya, José Manuel López-Aranda, José Federico Sánchez-Sevilla, Miguel Ángel Botella, and Victoriano Valpuesta

of difficulty in the analysis of polyploid species ( Röder et al., 1998 ). Simple sequence repeat (SSR) markers in the genus Fragaria L. have been developed from diploid species ( Bassil et al., 2006a ; Cipriani and Testolin, 2004 ; Hadonou et al

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Zuguo Cai, Wenfang Zeng, Liang Niu, Zhenhua Lu, Guochao Cui, Yunqin Zhu, Lei Pan, Yifeng Ding, and Zhiqiang Wang

/deletion events are also seen ( Decroocq et al., 2003 ). SSR markers are ideal for profiling as they are codominant and can display a large number of alleles per locus. In addition, assaying SSR markers is relatively simple and reproducible and is accomplished

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Rui Sun, Hui Li, Qiong Zhang, Dongmei Chen, Fengqiu Yang, Yongbo Zhao, Yi Wang, Yuepeng Han, Xinzhong Zhang, and Zhenhai Han

seedlings in a segregating population from ‘Jonathan’ × ‘Golden Delicious’ were studied in 2010 and 2011. The data were analyzed by the frequency distribution of phenotypes and assisted by SSR marker-based major gene mapping and QTL mapping for flesh

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Viji Sitther, Dapeng Zhang, Sadanand A. Dhekney, Donna L. Harris, Anand K. Yadav, and William R. Okie

traits, test parent–offspring and sibling relationships, and assess cross-transportability of SSR markers across related Prunus species. Materials and Methods Plant material. Thirty-seven peach accessions from the SEFTNRL collection were tested in this

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Riaz Ahmad, Miki Okada, Jeffrey L. Firestone, Chris R. Mallek, and Marie Jasieniuk

We isolated and characterized microsatellite loci in the ornamental pampas grass Cortaderia selloana (Schult. & Schult. f.) Asch. & Graebn. for purposes of identifying cultivars and assessing genetic relationships among cultivars. Small insert genomic libraries were enriched for dinucleotide (CT)n and (CA)n repeats. Ninety clones were sequenced of which 76% contained at least one microsatellite with a basic motif greater than six repeat units. Nine primer pairs amplified 10 polymorphic and putatively disomic loci, and were used to genotype 88 individuals representing 17 named cultivars and four selections. In total, 93 alleles were detected with a maximum of two to 19 per locus. Effective number of alleles varied from 1.3 to 9.5. Observed heterozygosity ranged from 0.07 to 0.81. The 10 microsatellite loci distinguished the majority of pampas grass cultivars. An unweighted pair group method with arithmetic mean (UPGMA) cluster analysis, based on proportion of shared alleles among individuals, revealed groups of cultivars corresponding to origin and morphological characteristics. With few exceptions, individuals of a single cultivar clustered together with moderate to strong bootstrap support (greater than 50%). Interestingly, `Pumila' from Europe and the United States formed separate clusters indicating independent origins. A large, diverse cluster with low bootstrap support consisted of selections and cultivars sold as seed, rather than potted or bare-root clonal plants. Primers designed for C. selloana amplified microsatellite loci in other Cortaderia Stapf species concordant with phylogenetic relationships among the species. Cross-amplification was 100% in C. jubata (Lemoine ex Carrière) Stapf; 77% in C. pilosa (d'Urv.) Hack. and C. rudiuscula Stapf; 66% in C. fulvida (Buch.) Zotov; and 55% in C. richardii (Endl.) Zotov and C. toetoe Zotov.

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Shuang Jiang, Haishan An, Xiaoqing Wang, Chunhui Shi, Jun Luo, and Yuanwen Teng

length that is repeated a variable number of times ( Zietkiewicz et al., 1994 ). SSR markers are characterized by a multiallelic nature, codominant inheritance, and good genome coverage ( Powell et al., 1996 ). SSR markers have been largely applied in

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Beibei Li, Xiucai Fan, Ying Zhang, Chonghuai Liu, and Jianfu Jiang

relationships among wild Chinese grape species. Klein et al. (2018) also used high-throughput sequencing to clarify evolutionary relationships among North American Vitis species. Because SSR markers show high levels of polymorphisms, reproducibility, and

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María José Arismendi, Patricio Hinrichsen, Ruben Almada, Paula Pimentel, Manuel Pinto, and Boris Sagredo

; Rojas et al., 2008 ). These have been widely used to characterize and classify commercial cultivars or estimate the genetic relationships among members of this genus. Among these, the microsatellite (SSR) markers are preferred because of their high level