Search Results

You are looking at 51 - 60 of 273 items for :

  • real-time PCR x
  • All content x
Clear All
Free access

Suping Zhou, Roger Sauve, and Fur-Chi Chen

constitutes one response to temperature stresses ( Provart et al., 2003 ). Real-time quantitative reverse transcriptase (qRT-PCR) has been used to quantify the level of transcripts present in plant organs ( Charrier et al., 2002 ; Lammers et al., 2001 ), thus

Free access

Wei Hu, Ju-Hua Liu, Xiao-Ying Yang, Jian-Bin Zhang, Cai-Hong Jia, Mei-Ying Li, Bi-Yu Xu, and Zhi-Qiang Jin

fruit as a template. The forward and reverse primers were designed according to GenBank as well as the standards for the real-time reverse transcriptase–polymerase chain reaction (real-time RT-PCR; TaKaRa Biotechnology, Dalian, China). To clone the

Free access

Jiyu Zhang, Min Wang, Zhenghai Mo, Gang Wang, and Zhongren Guo

, pistillate flowers, and fruitlets) and vegetative tissues (leaves and current-growth branches) of three varieties (‘Shaoxing’, ‘Pawnee’, and ‘Mahan’), were investigated by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). We

Free access

He Lisi, Su Jiale, Liu Xiaoqing, Li Chang, and Chen Shangping

the neighbor-joining method. Gene expression analysis. The real-time quantitative PCR (Q-PCR) was performed on a CFX96 TM Real-Time System (C1000 Thermal Cycler; Bio-Rad, Hercules, CA). Expression of an actin isoform (JN105299) was monitored as an

Free access

Hua Zhou, Fang-Yun Cheng, Jing Wu, and Chaoying He

) ( Tamura et al., 2011 ). Quantitative reverse transcription-polymerase chain reaction. Expression of the PsFT genes in tissues during development was evaluated using quantitative real time reverse transcription polymerase chain reaction (qRT-PCR), which

Free access

James M. Crosslin

.E. 2006 Development of a real-time, quantitative PCR for detection of the Columbia Basin potato purple top phytoplasma in plants and beet leafhoppers Plant Dis. 90 663 667 Gudmestad, N.C. Mallik, I. Pasche, J.S. Crosslin, J.M. 2008 First report of tobacco

Free access

James J. Polashock, Rajeev Arora, Yanhui Peng, Dhananjay Naik, and Lisa J. Rowland

(Applied Biosystems, Foster City, CA) was used for the qPCR reactions. Primers for qPCR were designed using Primer Express 3.0 software (Applied Biosystems) and are listed in Table 1 . Reactions were run on an Applied Biosystems 7500 real-time PCR machine

Full access

Misaki Ishibashi, Takeshi Nabe, Yoko Nitta, and Yuichi Uno

yield with fruits and anthers. Although these methods are compatible with subsequent reverse-transcription and/or real-time polymerase chain reaction (PCR) applications, their usefulness when followed by NGS like RNA-seq has not been reported. In cases

Free access

Akiko Watari, Toshio Hanada, Hisayo Yamane, Tomoya Esumi, Ryutaro Tao, Hideaki Yaegaki, Masami Yamaguchi, Kenji Beppu, and Ikuo Kataoka

SFB in pollen. Gene-specific primers for each S-RNase and SFB were designed using Primer Express (version 2.0; Applied Biosystems). The primer combinations used to amplify real-time RT-PCR products from SFB a , SFB b , SFB c , and SFB

Free access

Magaji G. Usman, Mohd Y. Rafii, Mohd Razi Ismail, Mohammad Abdul Malek, and Mohammad Abdul Latif

on a 1.5% agarose gel and using a spectrophotometer (Nanodrop 2000c; Thermo Fisher Scientific, Wilmington, DE). RNA with a 260/280 ratio of 1.8 to 2.0 was used for quantitative real-time polymerase chain reaction (PCR). The primers used for the