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Jianming Sun, Yiming Liu, Xianglin Li, and Bingru Huang

SDS–polyacrylamide gel electrophoresis, gels were washed in deionized distilled H 2 O three times for 15 min and stained with colloidal Coomassie Blue G-250 ( Neuhoff et al., 1988 ). The 2-DE gels were scanned using Image Scanner (Amersham Biosciences

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Kwanjai Pipatchartlearnwong, Akarapong Swatdipong, Supachai Vuttipongchaikij, and Somsak Apisitwanich

.30 min annealing temperature following Temp in Table 2 , 30 s extension step at 72 °C, and 8 min at 72 °C for the final extension step. The PCR products were separated and resolved by 6% polyacrylamide gel electrophoresis with silver staining. Primer

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Lanqing Wang, Yinfeng Li, Dehai Liu, Chaohui Zhang, Yuancheng Qi, Yuqian Gao, Jinwen Shen, and Liyou Qiu

spawn during storage were analyzed using polyacrylamide gel electrophoresis as described by Yan (1997) . The mean of five replicates was used in the result, except the basidiomata production experiment, which was performed with 20 replicates. Means were

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Tina P. Thomas, Madhurababu Kunta, John V. da Graça, Mamadou Sétamou, Mani Skaria, and Apurba Bhattacharya

viroid by polyacrylamide gel electrophoresis Proc. 9th Conf. IOCV 343 353 IOCV Riverside, CA Benton, R.J. Bowman, F.T. Fraser, L. Kebby, R.G. 1949 Stunting and scaly butt of citrus associated with

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Paolo Boccacci, Roberto Botta, and Mercè Rovira

Ahmad, Z. Daley, L.S. Menendez, M.A. Lagerstedt, H.B. 1987 Characterization of filbert ( Corylus ) species and cultivars using gradient polyacrylamide gel electrophoresis J. Environ. Hort. 5 11 16

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Tatsiana Espevig, Chenping Xu, Trygve S. Aamlid, Michelle DaCosta, and Bingru Huang

-dimension [isoelectric focusing (IEF)] and second-dimension [sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE)] separation of proteins were performed according to a procedure described by Xu et al. (2008 ). Briefly, immobilized pH gradient (IPG

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Ren-jun Feng, Li-li Zhang, Jing-yi Wang, Jin-mei Luo, Ming Peng, Jun-feng Qi, Yin-don Zhang, and Li-fang Lu

focusing, 400 µg of protein was loaded onto immobilized pH gradient strips (17 cm, pH 4–7). Sodium dodecyl sulfate polyacrylamide gel electrophoresis was performed with 12% gels. The protein spots were stained with colloidal Coomassie brilliant blue G-250

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Ben-Hong Wu, Ning Niu, Ji-Hu Li, and Shao-Hua Li

buffer containing 125 m m iodoacetamide and no DTT for another 15 min in the second. Two-dimension SDS–polyacrylamide gel electrophoresis was performed using an ETTAN DALTsix system (GE Healthcare). Equilibrated strips were placed on top of 12

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Ying Li, Liyi Zhang, Zhen Zhang, Peihua Cong, and Zong-Ming Cheng

extension at 72 °C for 1 min followed by a final extension at 72 °C for 10 min. The PCR products were resolved by 6% polyacrylamide gel electrophoresis. Before loading, the samples were denatured at 94 °C for 5 min. The gel was stained with silver nitrate

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Shigenori Yaguchi, Tetsuya Nakajima, Toshihisa Sumi, Naoki Yamauchi, and Masayoshi Shigyo

of the second PCR products was incubated for 2 h at 37 °C in a volume of 15 μL using 2 U of a restriction enzyme and subsequently resolved by 5% denaturing polyacrylamide gel electrophoresis (PAGE) with silver staining according to the procedure of