Search Results

You are looking at 51 - 60 of 91 items for :

  • "polyacrylamide gel electrophoresis" x
  • All content x
Clear All
Free access

Angela R. Davis, Penelope Perkins-Veazie, Richard Hassell, Amnon Levi, Stephen R. King, and Xingping Zhang

( C. sativus ) grafted on figleaf gourd ( C. ficifolia ) or on pumpkin ( C. maxima ). They found four proteins on sodium dodecylsulfate polyacrylamide gel electrophoresis gels in grafted scions that do not appear in control plants but matched the

Free access

Kwanjai Pipatchartlearnwong, Akarapong Swatdipong, Supachai Vuttipongchaikij, and Somsak Apisitwanich

.30 min annealing temperature following Temp in Table 2 , 30 s extension step at 72 °C, and 8 min at 72 °C for the final extension step. The PCR products were separated and resolved by 6% polyacrylamide gel electrophoresis with silver staining. Primer

Free access

Tina P. Thomas, Madhurababu Kunta, John V. da Graça, Mamadou Sétamou, Mani Skaria, and Apurba Bhattacharya

viroid by polyacrylamide gel electrophoresis Proc. 9th Conf. IOCV 343 353 IOCV Riverside, CA Benton, R.J. Bowman, F.T. Fraser, L. Kebby, R.G. 1949 Stunting and scaly butt of citrus associated with

Full access

Lanqing Wang, Yinfeng Li, Dehai Liu, Chaohui Zhang, Yuancheng Qi, Yuqian Gao, Jinwen Shen, and Liyou Qiu

spawn during storage were analyzed using polyacrylamide gel electrophoresis as described by Yan (1997) . The mean of five replicates was used in the result, except the basidiomata production experiment, which was performed with 20 replicates. Means were

Free access

Paolo Boccacci, Roberto Botta, and Mercè Rovira

Ahmad, Z. Daley, L.S. Menendez, M.A. Lagerstedt, H.B. 1987 Characterization of filbert ( Corylus ) species and cultivars using gradient polyacrylamide gel electrophoresis J. Environ. Hort. 5 11 16

Free access

Ren-jun Feng, Li-li Zhang, Jing-yi Wang, Jin-mei Luo, Ming Peng, Jun-feng Qi, Yin-don Zhang, and Li-fang Lu

focusing, 400 µg of protein was loaded onto immobilized pH gradient strips (17 cm, pH 4–7). Sodium dodecyl sulfate polyacrylamide gel electrophoresis was performed with 12% gels. The protein spots were stained with colloidal Coomassie brilliant blue G-250

Free access

Tatsiana Espevig, Chenping Xu, Trygve S. Aamlid, Michelle DaCosta, and Bingru Huang

-dimension [isoelectric focusing (IEF)] and second-dimension [sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE)] separation of proteins were performed according to a procedure described by Xu et al. (2008 ). Briefly, immobilized pH gradient (IPG

Free access

Ben-Hong Wu, Ning Niu, Ji-Hu Li, and Shao-Hua Li

buffer containing 125 m m iodoacetamide and no DTT for another 15 min in the second. Two-dimension SDS–polyacrylamide gel electrophoresis was performed using an ETTAN DALTsix system (GE Healthcare). Equilibrated strips were placed on top of 12

Free access

Jennifer A. Kimball, M. Carolina Zuleta, Matthew C. Martin, Kevin E. Kenworthy, Ambika Chandra, and Susana R. Milla-Lewis

were made according to Milla et al. (2005) . After amplification, AFLP fragments were denatured at 94 °C and separated by polyacrylamide gel electrophoresis on a LI-COR 4300 DNA Analyzer Sequencer (LI-COR Biosciences, Lincoln, NE) on 25-cm 8% v / v

Free access

Ying Li, Liyi Zhang, Zhen Zhang, Peihua Cong, and Zong-Ming Cheng

extension at 72 °C for 1 min followed by a final extension at 72 °C for 10 min. The PCR products were resolved by 6% polyacrylamide gel electrophoresis. Before loading, the samples were denatured at 94 °C for 5 min. The gel was stained with silver nitrate