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Ahmad Sattar Khan and Zora Singh

The short shelf life of japanese plum ( Prunus salicina Lindl.) and european plum ( Prunus domestica L.) fruit limits its export through sea freight. At 1 °C, japanese plum can be stored for only 3 to 5 weeks ( Navarro et al., 2005 ). Different

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Ralph Scorza, Laurene Levy, Vern Damsteegt, Luz Marcel Yepes, John Cordts, Ahmed Hadidi, Jerry Slightom, and Dennis Gonsalves

Transgenic plum plants expressing the papaya ringspot virus (PRV) coat protein (CP) were produced by Agrobacterium-mediated transformation of hypocotyl slices. Hypocotyl slices were cocultivated with Agrobacterium tumefaciens strain C58/Z707 containing the plasmid pGA482GG/CPPRV-4. This plasmid carries the PRVCP gene construct and chimeric NPTII and GUS genes. Shoots were regenerated on Murashige and Skoog salts, vitamins, 2% sucrose, 2.5 μm indolebutyric acid, 7.5 μm thidiazuron, and appropriate antibiotics for selection. Integration of the foreign genes was verified through kanamycin resistance, GUS assays, polymerase chain reaction (PCR), and Southern blot analyses. Four transgenic clones were identified. Three were vegetatively propagated and graft-inoculated with plum pox virus (PPV)-infected budwood in a quarantine, containment greenhouse. PPV infection was evaluated over a 2- to 4-year period through visual symptoms, enzyme-linked immunosorbent assay, and reverse transcriptase PCR assays. While most plants showed signs of infection and systemic spread of PPV within l-6 months, one plant appeared to delay the spread of virus and the appearance of disease symptoms. Virus spread was limited to basal portions of this plant up to 19 months postinoculation, but, after 32 months symptoms were evident and virus was detected throughout the plant. Our results suggest that heterologous protection with PRVCP, while having the potential to delay PPV symptoms and spread throughout plum plants, may not provide an adequate level of long-term resistance.

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Karen Inge Theron, Human Steenkamp, and Willem Jacobus Steyn

., 2011 ). Chemical thinning plums would reduce hand thinning substantially, but currently, chemical thinners available for stone fruit thinning are limited ( Seehuber et al., 2011 ). One chemical thinning approach for plums is to use gibberellins, e

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Dilip R. Panthee

‘Mountain Crown’ is a fresh-market plum tomato F 1 hybrid ( Solanum lycopersicum L.) developed by crossing NC 30P × NC 1 Plum. It is resistant to verticillium wilt ( Verticillium dahliae Kleb) (race 1) ( Ve/Ve gene), fusarium wilt ( Fusarium

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M.P. Bañados, M.S. Santiago, and C. Eterovic

The main form of nitrogen reserves during overwintering are amino acids and proteins. Specific proteins called bark storage proteins (BSP) have been characterized in many tree species. To identify BSPs in `O'Henry' peach, `Angeleno' plum, and `Early Burlat' cherry trees, samples of bark were collected from January through December 1993 from trees growing under field conditions in Santiago, Chile. SDS-PAGE analyses were used to characterize the seasonal variation on the protein pattern on the bark of those Prunus species. A 60 kDa BSP was identified in the bark of all three species, which corresponds to the main protein present in the bark during the winter. This protein may play an important role as a nitrogen reserve in these fruit trees.

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Peng Li, Weifeng Wu, Faxing Chen, Xinghui Liu, Yongan Lin, and Jianjun Chen

Plum [ Prunus salicina Lindl. (syn. Prunus triflora Roxb. or Prunus thibetica Franch.)], commonly known as chinese plum or japanese plum, is a diploid (2 n = 2 x = 16) fruit tree native to China. It is one of the most important stone fruit

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W.R. Okie

`Black Ruby' is the newest plum released by the USDA stone fruit breeding program at Byron. This variety has large, firm fruit that ripens in early July, about 1 week after `Santa Rosa'. Fruit has reddish-black skin and yellow flesh. Eating quality is very good. `Black Ruby' has an upright tree similar to `Santa Rosa', except that tree health and vigor are much better than `Santa Rosa'. USDA has been breeding plums for the humid Southeast for 30 years. Goals are to combine large, firm, high-quality fruit with a disease-resistant tree that will live 8 to 10 years. Most plum varieties are short-lived in our area due to disease caused by Xanthomonas, Pseudomonas, and Xylella. Most existing varieties adapted to our climate have fruit unsuitable for commercial production. Previous USDA releases include “green plum” types `Robusto' (1980) and `Segundo' (1984); a yellow plum, `Byrongold' (1985); a black shipping plum, `Explorer' (1980); and the blood-fleshed, high-quality `Rubysweet' (1989).

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Carolina Fernández, Jorge Pinochet, Daniel Esmenjaud, Maria Joao Gravato-Nobre, and Antonio Felipe

The influence of salinity and plant age on nematode reproduction was determined on two susceptible and six root-knot-nematode-resistant Prunus rootstocks inoculated with Meloidogyne incognita (Kofoid and White). Experiments were conducted under greenhouse conditions over 120 (plant age study) and 75 (salinity study) days. Following inoculation with 4000 nematodes per plant, susceptible 2-month-old GF-677 (Prunus persica L. Batsch. × P. dulcis Mill. Webb) and Montclar (P. persica) were affected significantly more than 1-year-old plants. Barrier (P. persica × P. davidiana Carr. Franch.) plantlets showed a partial loss of resistance in relation to older plants, suggesting that a root tissue maturation period is required for expression of full resistance. Nemared (P. persica); G × N No 22 (P. persica × P. dulcis); and the plums GF 8-1 (P. cerasifera Ehrh. × P. munsoniana Wight and Hedrick), PSM 101 (P. insititia L.), and P 2980 (P. cerasifera) maintained their high level of resistance or immunity, regardless of plant age. Nematode reproduction was higher in GF-677 rootstock in saline soil. Nemared and Barrier showed similar low galling and nematode reproduction in nonsaline and saline soil. PSM 101 immunity to M. incognita was not affected by soil condition.

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Unaroj Boonprakob, David H. Byrne, Charles J. Graham, W.R. Okie, Thomas Beckman, and Brian R. Smith

Diploid plums (Prunus L. sp.) and their progenitor species were characterized for randomly amplified polymorphic DNA polymorphisms. Bootstrap analysis indicated the variance of genetic similarities differed little when the sample size was >80 markers. Two species from China (Prunus salicina Lindl. and P. simonii Carr.) and one species from Europe (P. cerasifera Ehrh.) contributed the bulk (72% to 90%) of the genetic background to the cultivated diploid plum. The southeastern plum gene pool was more diverse than those from California, Florida, or South Africa because of the greater contribution of P. cerasifera and P. angustifolia Marsh. to its genetic background.

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James D. Dutcher, Gerard W. Krewer, and Benjamin G. Mullinix Jr.

Observations in controlled field experiments over 5 years indicated that imidacloprid, applied as a soil drench around the trunks of peach (Prunus persica), nectarine (P. persica var. nectarine) and japanese plum (P. salicinia) trees at planting and in the early spring and mid-summer for two subsequent seasons (0.7 g/tree a.i.), slowed the development of symptoms of phony peach disease (PPD) and plum leaf scald (PLS) (Xylella fastidiosa) in the trees. After 3.5 years, the percentage of peach trees showing PPD symptoms was 8.5% for the imidacloprid-treated trees compared to 34.3% for untreated trees. After 4.5 years, the percentage of peach trees showing PPD symptoms was 13.1% in the treated trees and 71.4% in the untreated trees. After 3.5 years, nectarine trees in untreated and treated plots showed PPD symptoms in 8.3% and 0.9% of the trees, respectively. After 4.5 years, PPD symptoms in nectarine were found in 32.3% of the untreated trees and 8.5% of the treated trees. Development of PLS disease in plum was also slowed by the trunk drench with imidacloprid in two japanese plum varieties. After 3.5 years, dieback was observed in 55% of the twigs of untreated and 23% of the twigs of treated trees of `Au Rosa' plum and 33% of the twigs of untreated and 12% of the twigs of treated trees of `Santa Rosa' plum.