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Harbans L. Bhardwaj and Anwar A. Hamama

with sprouts of alfalfa, brussels sprouts, mungbean, and radish for overall nutritional quality. Although it is well established that sprouts from seeds of cruciferous plants ( Brassica sp.) such as brussels sprouts, broccoli, and cauliflower are rich

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Joseph Krystel, Huawei Liu, John Hartung, and Ed Stover

plasmid vector pUSHRL ( Supplemental Fig. 1 ). The pUSHRL plasmid is a binary vector suitable for agrobacterium ( Agrobacterium tumefaciens ) mediated transformation and expression of a cloned sequence under control of a tandem Cauliflower mosaic virus

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Jose R.A. Santos and Daniel I. Leskovar

Poor seedling emergence and stunted growth were observed in cauliflower (Brassica oleracea L., Botrytis group) and cabbage (Brassica oleracea L., Capitata group) crops when planted after three consecutive monocrops of broccoli (Brassica oleracea L., Italica group). This study was conducted to assess seed, seedling, and plant growth responses of broccoli, cabbage, and cauliflower to broccoli residue (leaves, stem + roots, and whole plant) extracts, broccoli residue incorporation, or soil previously cropped to broccoli. Osmotic potential, electrical conductivity, and pH of extracts were measured, rate (T50) and total germination were determined. Filter-sterilized leaf extract delayed T50(7.5 d) and reduced total germination (22%) of cauliflower compared to broccoli or cabbage. Similarly, plant height, shoot dry weight, and leaf area of cauliflower were significantly reduced when grown on broccoli soil in the greenhouse. Cabbage and cauliflower had low total marketable yields with more culled heads when grown in the field previously cropped to broccoli. Therefore, a potential growth inhibition of cabbage and cauliflower exists when following a continous cropping of broccoli.

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Hai-nan Liu, Jian-rong Feng, Xiao-fang Liu, Wen-hui Li, Wen-juan Lv, and Ming Luo

fragment, CaMV 35S = cauliflower mosaic virus 35S promoter, NOS-ter = nopaline synthase terminator, NPT II = neomycin phosphotransferase II gene (kanamycin resistance), GUS = β-glucuronidase gene. Eco RV, Hind III, Kpn I, Sal I, Xba I, Sac I

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Carol J. Lovatt

/acre, 46% N, ≤ 0.25% biuret) and boron [B (1.4 lb/acre B as sodium tetraborate)] applied at the cauliflower stage of inflorescence development, about the time of gametogenesis (pollen, ovule, and egg formation) ( Salazar-García et al., 1998 ), increased

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Guo-Qing Song, Kenneth C. Sink, Peter W. Callow, Rebecca Baughan, and James F. Hancock

cloning sites plus bar∷nos . Subsequently, a 5′- Bam HI (blunted)-bar-nos- Eco RI-3′ fragment was ligated with a 1.1-kb 5′- Xba I- Bam HI (blunted)-3′ fragment, derived from pMS34b, containing the cauliflower mosaic virus (CaMV) 34S promoter. The 5′- Xba

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G. Préstamo and P. Manzano

The various isozymes of peroxidase of a range of vegetables and kiwifruit were compared using sodium dodecyl sulfate polyacrylamide gel electrophoresis followed by specific activity staining. Peroxidase isozymes were determined in potato (Solanum tuberosum L.), carrot (Daucus carota L.), tomato (Lycopersicon esculentum Mill.), kiwifruit [Actinidia deliciosa (A. Chev.) C.F. Liang et A.R. Ferguson], cauliflower [Brassica oleracea (Botrytis group)], green beans (Phaseolus vulgaris L.), and horseradish (Armoracia rusticana Gaertn, Mey Scherb.). There was only one isozyme in cauliflower (70 kDa), two in kiwifruit (45-43 kDa), and a range of isozymes (120-36 kDa) in horseradish. Ascorbic acid inhibited peroxidase activity in the extracts.

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Sven E. Svenson, Robert B. McReynolds, and Wes A. Deuel

Field evaluation of meadowfoam (Limnanthes alba) seedmeal as a soil amendment to control clubroot caused by Plasmodiophora brassicae was conducted using cauliflower seedlings and seeded mustard grown in naturally infested soils. Ionic surfactant drench was applied as a comparative treatment. Meadowfoam seedmeal (MSM) incorporation at a rate of 15,000 kg·ha-1 resulted in greatest control of clubroot incidence and severity. This treatment also resulted in significantly greater fresh weight yield of cauliflower compared to controls and surfactant drench application. Some phytotoxicity symptoms were observed at high rate (30,000 kg·ha-1) of MSM treatment. MSM glucosinolate products may have an important role in regulating plant-pathogen interactions.

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Kelly A. Zarka, Ria Greyling, Inge Gazendam, Dean Olefse, Kimberly Felcher, Gurling Bothma, Johan Brink, Hector Quemada, and David S. Douches

Syngenta (Basel, Switzerland). In this vector, the cry1Ia1 gene is under the control of a 35S cauliflower mosaic virus (CaMV) promoter ( Fig. 1 ) and the nptII gene is present as a selectable marker. The pSPUD5 vector was transformed into A

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James J. Polashock, Rajeev Arora, Yanhui Peng, Dhananjay Naik, and Lisa J. Rowland

was digested with Hind III and Eco RI, yielding the BB- CBF fragment downstream of the cauliflower mosaic virus 35S promoter and enhancer and ended by the 35S polyA signal. This fragment was gel purified and ligated into the Agrobacterium