`Flying Dragon' Poncirus trifoliata L. Raf. is a dwarfing rootstock for citrus. Inheritance of dwarfing ability was studied in a population of open-pollinated seedlings of `Flying Dragon'. Molecular marker genotypes suggest that all seedlings originated from selfing. Progeny seedlings were budded with `Cutter Valencia' orange and planted in the field to evaluate the dwarfing effect of the seedling rootstock. At 5 years after planting, rankit analysis of the frequency distributions of trunk cross-sectional area and canopy volume suggested the presence of two overlapping distributions of 34 dwarf trees and 7 nondwarf. This ratio is consistent with inheritance of rootstock dwarfing as a single dominant gene for which `Flying Dragon' is heterozygous. Two morphological characteristics of `Flying Dragon', curved thorns and twisted trunk growth, were closely linked to, or pleiotropic effects of, the dwarfing gene. Bulked segregant analysis was used to identify three RAPD markers linked to the dwarfing gene. `Flying Dragon' was identical to nondwarfing cultivars of trifoliate orange at 40 homozygous and heterozygous isozyme and RFLP markers; therefore, it is likely that `Flying Dragon' originated as a mutant of a nondwarfing genotype and has not undergone sexual recombination since this event.
Frank Suozhan Cheng and Mikeal L. Roose
Mikeal L. Roose, Frank Suozhan Cheng, and Claire T. Federici
The `Flying Dragon' cultivar of Poncirus trifoliata L. Raf. is a strongly dwarfing rootstock for Citrus cultivars, reducing canopy volume of 9 year-old `Valencia' orange trees to 1/3 that of trees on standard rootstocks Open-pollinated seed of `Flying Dragon' was screened with isozyme markers to distinguish zygotic from nucellar (apomictic) seedlings. All zygotics had genotypes consistent with an origin by self-pollination. Zygotic seedlings were budded with `Valencia' orange scion and planted in the field. Of 46 progeny evaluated as rootstocks, 35 produced small trees similar to those on nucellar `Flying Dragon' and 11 produced large trees. This ratio is consistent with the 3:1 segregation expected for a single dominant gene. The dwarfing gene was closely linked, or pleiotropic with a gene causing curved thorns and stems. Several RAPD markers close to the dwarfing gene were identified with bulked segregant analysis. `Flying Dragon' apparently originated as a mutation because it had au identical genotype to non-dwarfing strains of trifoliate orange at all 38 isozyme and RFLP markers tested
J.G. Williamson and R.L. Darnell
Mature `Sharpblue' southern highbush and `Beckyblue' rabbiteye blueberry plants were mechanically pruned at two heights on three dates after fruit harvest during the 1994 growing season. No pruning had occurred for at least 3 years (`Sharpblue') or 5 years (`Beckyblue') before initiating experiment. Pruning heights were 45 and 85 cm and nonpruned for `Sharpblue' and 45 and 95 cm and nonpruned for `Beckyblue'. Pruning dates were 3, 6, and 9 weeks after peak harvest for each cultivar. Regrowth was measured in Mar. 1995 before initiation of spring growth. Pruning `Sharpblue' bushes to 45 cm increased new shoot number and mean and total shoot length but decreased fruit yield compared to the 90-cm pruning treatment. No difference in yield occurred between the 90-cm pruning treatment and the nonpruned control. As time between fruit harvest and pruning increased, new shoot number, mean and total shoot length, plant height, canopy volume, and fruit yield decreased. There was no difference in yield between the earliest pruning treatment and the control. For `Beckyblue', mean and total shoot length of regrowth and flower bud density decreased with increasing time from harvest to pruning. Yield data for `Beckyblue' were not collected in 1995 because of gall midge infestation.
Curt R. Rom, R.C. Rom, and R. Bourne
`Redhaven' trees on 10 rootstocks planted in 1984 were annually evaluated for growth and cropping as part of the NC-140 national cooperative rootstock troial. All trees on Citation have died, 60 % of trees on GF-43 have died and only a single tree of Lovell, Halford, and GF-677 have died. Trees on Damas GF-1869 and GF-655.2 had significantly more root suckers than other trees. Redhaven own-root, Halford and GF-677 were largest in height, spread, canopy volume or TCSA while the smallest trees were GF-43, Damas, and GF-655.2. Damas, GF-43 and GF-655.2 bloomed 3-4 days before trees on Lovell. Fruit on Redhaven own-root matured 4 days before fruit on Lovell while fruit on Halford, GF-677 and GF-43 ripened 2 days later than Lovell. Trees on Halford had the highest annual yield and accumulated yield while GF-655.2, Damas and GF-43 had the lowest yields. Redhaven own-root and Halford had the highest yield efficiencies (kg/cm2TCSA). Trees on Lovell consistently produced the largest individual fruit size.
R.C. Beeson Jr.
Three species of woody ornamentals, Viburnum odoratissimum Ker Gawl, Ligustrum japonicum Thunb., and Rhaphiolepis indica Lindl. were transplanted from 3.8-L into 11.4-L containers and grown for 6 months while irrigated with overhead sprinkler irrigation. Irrigation regimes imposed consisted of an 18-mm-daily control and irrigation to saturation based on 20%, 40%, 60%, and 80% deficits in plant available water [management allowed deficits (MAD)]. Based on different evaluation methods, recommendations of 20%, 20%, and 40% MAD are supported for V. odoratissimum, L. japonica, and R. indica, respectively, for commercial production. Comparisons of plant growth rates, supplied water, and conversion of transpiration to shoot biomass are discussed among irrigation regimes within each species. Comparisons of cumulative actual evapotranspiration (ETA) to either shoot dry mass or canopy volume were linear and highly correlated. Results indicated there were minimum cumulative ETA volumes required for plants to obtain a specific size. This suggests that irrigation regimes that restrict daily ETA will prolong production times and may increase supplemental irrigation requirements. Thus the hypothesis that restrictive irrigation regimes will reduce irrigation requirements to produce container plants is false due to the strong relationship between cumulative ETA and plant size.
Thomas A. Obreza and Robert E. Rouse
The growth response of newly-planted 'Hamlin' orange (“Citrus sinensis L. Osbeck) on Carrizo citrange (C. sinensis × Poncirus trifoliata L. Raf.) trees to N-P-K fertilizer rates was studied to determine the minimum fertilizer required to bring trees into maximum early production. The highest fertilizer rate applied was 2.72, 5.45, and 8.17 kg·tree-1 of an 8-1.8-6.6 N-P-K fertilizer in 1989, 1990, and 1991, respectively. Additional fertilizer treatments equalled 50, 25, or 13% of the maximum rate. The response of trunk cross-sectional area, tree canopy volume, and fruit yield to fertilizer rate was described by a linear plateau model. The model predicted a fruit yield of 22.6 kg·tree-1 at the estimated critical rate of 48% of maximum. Fruit yield at 50% of maximum rate averaged 21.2 kg·tree. As fertilizer rate increased, total soluble solids (TSS) in juice and ratio (TSS:acid) decreased, but weight per fruit and TSS per tree increased. A fruit yield above 21 kg·tree-1 from 31-month-old trees was indicative of vigorous growth.
Ashok K. Alva
The aim of this study was to investigate soil pH and copper (Cu) interactions affecting Cu phytotoxicity to young citrus trees on different rootstocks. Hamlin oranges on either Carrizo citrange, sour orange, or rough lemon rootstocks were grown on Candler fine sand at varying soil pH (5.0, 5.5, 6.0, 6.5) without additional Cu or soil applied Cu (liquid form; 240 kg Cu/ha; nine pre- and five post-planting applications over a period of 43 months). Increasing soil pH increased tree height, canopy volume and trunk diameter of trees on all three rootstocks, regardless of Cu treatments. Tree growth response to an increase in soil pH was greater in Cu amended as compared to unamended treatments. Response to pH increase above 6.0 was marginal as compared to that for pH increase from 5.0 to 6.0. Leaf Cu concentrations showed negligible differences in response to Cu treatments; however, Cu concentrations in fibrous roots increased by 126 to 152% in Cu amended as compared to unamended treatments.
Stephen S. Miller and Ross E. Byers
When temperatures reach -26 °C and lower, even for brief periods of time, damage to fruit buds and woody tissue of the peach tree is common. Low temperature injury on peach can lead to bark damage, gummosis, increased incidence of perennial canker, partial or complete crop losses, reduced shoot growth and/or tree death. In Jan. 1994 the Eastern Panhandle of West Virginia and surrounding states experienced three successive nights of temperatures at -28 °C or lower. Beginning in Apr. 1994, 7-year-old `Blake'/Lovell peach trees were subjected to four pruning levels (none, light, heavy, and dehorned) each at three times (April, May, and June) in a replicated factorial arrangement. Specific pruning treatments were applied only in 1994; a local commercially recommended level and time of pruning were applied to all trees from 1995 through 1998. Treatments had a significant effect on canopy volume and fruit yields. Trees receiving no pruning or dehorned trees and trees pruned in June had lower yields in 1995 than trees pruned in April or May or trees receiving a light or heavy pruning. These treatments also produced fewer large fruit at harvest. Lower yields and smaller fruit led to reduced dollar returns per hectare in 1995. Yields from 1996 through 1998 were lower for trees that were dehorned pruned in 1994 although there were little or no differences in fruit sizes between treatments. Time and/or level of pruning had effects on the number of cankers and number of large (>5.1 cm) cankers.
Mebelo Mataa and Shigeto Tominaga
The effects of root restriction, induced by root restriction bags, was evaluated on `Yoshida' Ponkan mandarin (Citrus reticulata Blanco). Trees were planted in 0.02-m3 volume root wrap bags (RWBs), which were made from woven polystyrene fiber, or root control bags (RCBs) made from nonwoven UV-stabilized Duon polystyrene fibre with plastic bottoms. A direct soil planted, nonrestricted root treatment (DPC) was included as a control. After 3 years, reductions in height (14% to 29%), canopy volume (66% to 43%), girth (10% to 22%), and leaf area (8% to 12%) were recorded in both of the root restriction treatments. Greater reductions occurred in the RWB treatment. Photosynthesis, transpiration, water potential, and leaf carbohydrate content were not affected by root restriction although soil moisture content was lower in the root restricted treatments. Fruiting efficiency (i.e., number of fruit per unit volume of tree canopy) improved only in the RWB treatment over the control. Total soluble solids and the fruit color index were enhanced by root restriction.
Jim Syvertsen and M.L. Smith
Effects of nitrogen (N) rate and rootstock on tree growth, fruit yield, evapotranspiration, N uptake, and N leaching were measured over a 2-year period. Four-year-old `Redblush' grapefruit trees on either sour orange (SO), a relatively slow-growing rootstock, or `Volkamer' lemon (VL), a more-vigorous rootstock, were transplanted into 7.9-m3 drainage lysimeter tanks filled with native sand and fertilized at three N rates. N rates averaged from about 14% to 136% of the recommended rate when trees were 5 and 6 years old. More N leached below trees on SO as trees on VL had greater N uptake efficiency. Canopy volume and leaf N concentration increased with N rate, but rootstock had no effect on leaf N. Fruit yield of trees on SO was not affected by N rate, but high N increased water use and yield for larger trees on VL. Canopy growth or yield per volume of water used (water use efficiency) was lowest at low N, but N use efficiency was highest at the low N rates.