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Jianjun Chen, Lijia Li, and Ying Wang

30 s at 94 °C, 50 s annealing at 47 °C, and 45 s at 70 °C with a final extension step at 72 °C for 10 min. Purified PCR products (E. Z. N. A. ® Gel Extraction Kit; Omega Bio-tek, Norcross, GA) were ligated into the pMD18-T plasmid vector (TaKaRa Bio

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T. Casey Barickman, Dean A. Kopsell, and Carl E. Sams

average of 900 μmol·m −2 ·s −1 over the entire photoperiod. Light intensity readings were taken at 1.22 m off the ground. At 30 d after seeding, the plantlets were transferred to 11-L Dutch pots (Tek Supply, Dyersville, IA) filled with Sunshine ® Pro

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Craig J. Frey, Xin Zhao, Jeffrey K. Brecht, Dustin M. Huff, and Zachary E. Black

air temperatures were <50 °F, and all sides remained open when air temperatures were >80 °F. For late-season growth, shadecloth (Aluminet I 40% Greenhouse Shadecloth; Green-Tek, Inc., Dinuba, CA) was applied to the high tunnels as daily high

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T. Casey Barickman, Dean A. Kopsell, and Carl E. Sams

photoperiod. Light intensity readings were taken at 1.22 m off the ground. At 30 d after seeding, the plantlets were transferred to 11-L Dutch pots (Tek Supply, Dyersville, IA) filled with Sunshine Pro Soil Conditioner (Sungro Horticulture, Agawam, MA). Tomato

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Itani Tshivhandekano, Fhatuwani Nixwell Mudau, and Thilivhali Emmanuel Tshikalange

absorbencies were read with an enzyme-linked immunosorbent assay (ELISA) plate reader (KC junior version 4.0; Bio Tek Instruments) at 515 nm, and the absorbance values were analyzed with Graph Pad Prism 4.0. For antioxidant activity analyses, the sum of squares

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Ran Chen, Weitao Jiang, Haiyan Wang, Fengbing Pan, Hai Fan, Xuesen Chen, Xiang Shen, Chengmiao Yin, and Zhiquan Mao

sample. Soil microbial community structure The extraction and purification of total DNA from the sample genome was performed per the instructions of the E.Z.N.A. Soil DNA Kit (Omega Bio-Tek, Norcross, GA). The CFX96 TM Connect real-time quantitative PCR

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Gerardo H. Nunez, Hilda Patricia Rodríguez-Armenta, Rebecca L. Darnell, and James W. Olmstead

found where size-adjusted and unadjusted data were used simultaneously ( Johnson et al., 2000 ; Khan et al., 2012 ). Leaf tissue from each seedling was used to extract total genomic DNA using the E-Z 96 ® Plant DNA Kit ™ (Omega Bio-Tek, Norcross, GA

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Craig J. Frey, Xin Zhao, Jeffrey K. Brecht, Dustin M. Huff, and Zachary E. Black

until temperatures were >27 °C. When daily high temperatures in the open field were sustained at >32 °C, Aluminet I 40% Greenhouse Shadecloth (Green-Tek Inc., Dinuba, CA) was applied to the high tunnels (2 May 2016 and 17 Apr. 2017). Disease management

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Suzanne Stone, George Boyhan, and Cecilia McGregor

collected in 1.5-mL microtubes were ground using 5-mm steel beads in a TissueLyser (Qiagen, Inc., Valencia, CA) for 30 s then DNA was extracted using the E-Z 96 Plant DNA Kit (Omega Bio-Tek, Norcross, GA). Extracted DNA was quantified using the Tecan

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Julia M. Harshman, Wayne M. Jurick II, Kim S. Lewers, Shiow Y. Wang, and Christopher S. Walsh

of a robotic eight-channel liquid handling system. A microplate fluorescence reader (FL800; Bio-Tek Instruments, Winooski, VT) was used with fluorescence filters for an excitation wavelength of 485 ± 20 nm and an emission wavelength of 530 ± 25 nm