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Binoy Babu, Gary Knox, Mathews L. Paret, and Francisco M. Ochoa-Corona

tissues ( Babu et al., 2016b ; J. Olson, personal communication). DEVELOPMENT OF REAL-TIME RT-PCR DIAGNOSTIC TOOLS Nucleic acid–based methods including end-point polymerase chain reaction (PCR) and RT-PCR are widely used in virus detection ( Mackay et al

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Zongchang Xu, Meng Wang, Jinhui Zhou, Han Liu, Chengsheng Zhang, and Yiqiang Li

of the primer pairs was also validated by melting-curve analyses following amplification by qRT-PCR. Amplification efficiency test of qRT-PCR primer pairs. We performed qRT-PCR analysis on an Applied Biosystems 7500 Real-Time PCR system (Thermo Fisher

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Ute Albrecht, David G. Hall, and Kim D. Bowman

trees (Sept. 2009 to June 2010) and in greenhouse-grown plants, real-time PCR assays were performed using primers HLBas and HLBr and probe HLBp developed by Li et al. (2006) . For normalization, all samples were assayed using primers COXf and COXr and

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Shaolan Yang, Changjie Xu, Bo Zhang, Xian Li, and Kunsong Chen

primer sets used for real-time PCR analysis were designed according to the sequences of 3′-untranslated regions (UTR) of two individual members obtained in the current study using software Primer Premier 5.0 (Premier Biosoft Intl., Palo Alto, Calif.). The

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Ute Albrecht and Kim D. Bowman

PCR assays were performed using primers HLBas (5′-TCGAGCGCGTATGCAATACG-3′) and HLBr (5′-GCGTTATCCCGTAGAAAAAGGTAG 3′) and probe HLBp (5′-AGACGGGTGAGTAACGCG-3′) developed by Li et al. (2006) . Amplifications were performed using an ABI 7500 real-time

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Ute Albrecht and Kim D. Bowman

% agarose gels (Amresco, Solon, OH) for 70 min at 5 to 6 V·cm −1 , stained with ethidium bromide, and visualized under ultraviolet light (Fluor S Imaging System; Bio-Rad Laboratories, Hercules, CA). A comparison with quantitative real-time PCR using primers

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Li-Xiao Yao, Yong-Rui He, Hai-Fang Fan, Lan-Zhen Xu, Tian-Gang Lei, Xiu-Ping Zou, Ai-Hong Peng, Qiang Li, and Shan-Chun Chen

, transmembrane regions, and oxidoreductase signature motifs ( Andaluz et al., 2009 ; Mukherjee et al., 2006 ). In addition, CjFRO gene expression was confirmed via real-time PCR in different tissues and in conditions of Fe sufficiency and deficiency. The

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Xiucai Fan, Renzong Zhao, Qianqian Wang, Chonghuai Liu, and Jinggui Fang

)-mass spectrometry (MS), characterize the MybA-related genes at the color locus via capillary electrophoresis and quantitative real-time polymerase chain reaction (qRT-PCR) in Kyoho and its derivatives to identify the germplasm resources with higher total anthocyanin

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Jing Ma, Zheng Li, Bin Wang, Shunzhao Sui, and Mingyang Li

reaction analysis. Equal amounts of DNA-free RNA (5 μg) from different tissues were reverse-transcribed using the PrimeScript RT Reagent Kit with gDNA Eraser (TaKaRa, Otsu, Japan). Real-time polymerase chain reaction (PCR) was performed in triplicate on 10

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Xianqin Qiu, Hongying Jian, Qigang Wang, Kaixue Tang, and Manzhu Bao

will most likely lead to the identification of genes relevant to new biological processes or that are implicated in disease ( Vandesompele et al., 2002 ). Real-time quantitative PCR (RT-qPCR) has become the gold standard for the quantitative analysis of