occurring, the extent of production, and the primary blueberry species responsible for this production. Chapter 2 first reviewed the taxonomy of the blueberry. Breeding efforts past and present were discussed next, followed by a description of
Duane W. Greene
Ann Marie Connor, James J. Luby, and Cindy B.S. Tong
Variation in antioxidant activity (AA), total phenolic content (TPH), and total anthocyanin content (ACY) was examined in 1998 and 1999 in fruit of 52 (49 blue-fruited and 3 pink-fruited) genotypes from a blueberry breeding population. The species ancestry included Vaccinium corymbosum L. (northern highbush blueberry), V. angustifolium Ait. (lowbush blueberry), V. constablaei Gray (mountain highbush blueberry), V. ashei Reade (rabbiteye blueberry), and V. myrtilloides Michx. (lowbush blueberry). Using a methyl linoleate oxidation assay (MeLO) on acidified methanolic extracts of the berries, a 5-fold variation was found in AA in 1998 and a 3-fold variation in 1999 among the blue-fruited genotypes. Analyses of variance (ANOVA) revealed variation among genotypes (P < 0.0001) in single and combined years, regardless of inclusion of pink-fruited selections and adjustment for berry size. While mean AA of all genotypes did not change between the 2 years, ranking of some genotypes for AA changed significantly between 1998 and 1999. Of the 10 genotypes that demonstrated the highest AA in 1998, four were among the 10 genotypes that demonstrated highest AA in 1999. Similarly, of the 15 genotypes with the highest AA, 10 were the same both years. As with AA, mean TPH of all genotypes did not change between years and ANOVA demonstrated genotypic variation regardless of adjustment for berry size/weight or exclusion of pink-fruited selections. Changes in genotype rank occurred between years. The difference in TPH between lowest- and highest-ranking blue-fruited genotypes was ≈2.6-fold in both 1998 and 1999. Seven of the 10 highest-ranking genotypes were the same both years and TPH correlated with AA (r = 0.92, P < 0.01) on a genotype mean basis for combined years. ACY correlated less well with AA (r = 0.73, P < 0.01 for combined years). When genotypes were categorized into six groups according to species ancestry, V. myrtilloides and V. constablaei × V. ashei crosses ranked highest and second highest, respectively, for AA in both years. The groups comprised of V. corymbosum genotypes, V. angustifolium genotypes, and those with both V. corymbosum and V. angustifolium in their lineage were indistinguishable from each other. Samples from some of the genotypes were analyzed for oxygen radical absorbance capacity and ferric-reducing antioxidant power, and these aqueous-based antioxidant assays correlated well with the lipid emulsion-based MeLO (all r ≥ 0.90, P < 0.01). The three antioxidant assays may be equally useful for screening in a blueberry breeding program and the choice of assay may depend on the goal of the program and the resources available.
Les Padley Jr. and Paul Lyrene
Oral Session 14—Fruit Breeding 28 July 2006, 4:15–5:15 p.m. Bayside A Moderator: David Byrne
Jeffrey G. Williamson and William O. Cline
following topics are discussed: 1) use of sparkleberry ( V. arboreum ) in breeding to improve traits of southern highbush blueberry for mechanical harvesting; 2) effects of mechanical harvesting on postharvest quality of southern highbush blueberry; 3
W.A. Erb, A.D. Draper, and H.J. Swartz
Progenies and clones of interspecific hybrid blueberries were evaluated for annual fraction of canopy volume (FCYV) and for difference in fraction of canopy volume between control and stressed plants [FCYV(C) - FCYV(S)] in a moderate water-deficit environment. The FCYV(C) - FCYV(S) data were used to determine combining ability effects. In addition, physiological processes of attached leaves of the clones were monitored with a portable photosynthesis apparatus. Specific combining ability (SCA) effects were significant for FCYV(C) - FCYV(S). The clone with the lowest mean for FCYV(C) - FCYV(S) was US75, a hybrid of Vaccinium darrowi Camp × V. corymbosum L. Clone JU64 (V. myrsinites Lamark × V. angustifolium Aiton) also had a low FCYV(C) - FCYV(S) mean, and its two progenies (JU64 × JU11 and G362 × JU64) had low progeny means. Stomatal conductance was lowered when blueberries were exposed to atmospheric and/or soil moisture stress that resulted in lower transpiration and photosynthesis and increased or equal water-use efficiencies (WUE). Blueberry plants adjusted to moisture stress as the season progressed by lowering stomatal conductance and increasing WUE. In particular, stressed plants of US75 and JU64 had equal or higher WUE values than control plants. US226 was the most drought-susceptible clone in the study, and its stomata did not appear to be as responsive to moisture stress as the other clones. Breeding for higher WUE in a dry environment appears possible with the germplasm used in this study.
S.L. Noffsinger, A.D. Draper, and C.L. Gupton
Although southern highbush (Vaccinium sp.) is replacing rabbiteye (Vaccinium ashei L.) blueberry, rabbiteye will continue to be grown on marginal soils of the southeastern United States. Dwarfism or short, compact growth habit is a trait that could be used to reduce labor costs in rabbiteye blueberry production. Parental backgrounds, and flowering and fruit traits were studied in seven Mississippi (MS) and five Georgia (T) selections. Six of the MS selections are available for propagation and bloom late enough that cold damage should not be a problem. Four (MS63, MS454, MS546, MS891) of the six have acceptable fruit quality and will be used in breeding. Ethel and MS134 were the only known dwarf ancestors, with Ethel, Myers, Black Giant, and Tifblue (Ethel × Clara) dominating the parental background. Based on the variation in growth habit and ancestries, it would appear that Ethel has several genes for dwarfism and multiple allelic interactions are involved, similar to what Garvey and Lyrene found (1987). Future breeding will include crosses of MS63, MS454, MS546, and MS891 with germplasm outside of the common ancestors, to broaden the genetic base of the dwarf rabbiteyes.
Mark K. Ehlenfeldt, Filmore I. Meredith, and James R. Ballington
The fruit of six highbush (Vaccinium corymbosum L.) cultivars and eight rabbiteye (V. ashei Reade) cultivars and selections were evaluated by high-performance liquid chromatography for levels of the commonly found organic acids, citric, malic, succinic, and quinic. The two cultivar groups possessed distinctive patterns of relative organic acid proportions that could unambiguously separate pure rabbiteye and highbush clones in a principal component analysis. Highbush clones were characterized by high citric acid content, with percentages averaging 75% (range 38% to 90%). Succinic acid was the second most plentiful acid, averaging 17%. In contrast, rabbiteye cultivars and selections contained 10% citric acid, and no clone had >22%. Succinic acid and malic acid were found in greater quantities than in highbush, averaging 50% and 34%, respectively. Analysis of the fruit of seven albino-fruited highbush selections exhibited a profile similar to standard highbush cultivars, but with a citric acid average of <50%, and proportionally greater amounts of succinic and quinic acids. Given the differences in sensory quality of these four acids, it is likely that acid partitioning patterns can largely account for some of the perceived flavor differences between rabbiteye and highbush blueberries. Because several current breeding efforts involve hybridization between highbush and rabbiteye blueberries, a consideration of acid composition of breeding parents maybe worthwhile.
Chad E. Finn, Bernadine C. Strik, Theodore A. Mackey, Kim E. Hummer, and Robert R. Martin
-ARS) breeding program in Corvallis, OR, released in cooperation with Oregon State University’s Agricultural Experiment Station ( Finn, 2014 ). ‘Perpetua’ is the first strongly repeat fruiting (remontant, off-season, perpetual flowering) blueberry developed from
D. Scott NeSmith
). Vaccinium darrowii is typically compact in the wild and is often evergreen, whereas rabbiteye blueberries are typically deciduous and highly vigorous. Both V. darrowii and V. virgatum have been used for many years in breeding programs to develop hybrids
Andrew Raymond Jamieson
The expansion of the highbush blueberry (principally Vaccinium corymbosum L.) industry worldwide is supported by several successful breeding programs that have created cultivars with improvements in fruit size and firmness while retaining a