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the Genetics Department, Faculty of Agriculture, during 2014–15. Table 1. List of accessions scientific names, collection site, and country of origin. Protein extraction and electrophoresis. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) was carried

state for non-denaturing polyacrylamide gel electrophoresis (PAGE) with evaluation of in gel PG activity or desalted with a Plus One 2-D Clean-Up Kit (GE Healthcare Europe, Milan, Italy) for sodium dodecyl sulfate (SDS)-PAGE. Protein content was

595 nm in a spectrophotometer (Novaspec Plus; Amersham BioSciences, Piscataway, NJ). The remaining supernatant was retained for sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. SDS-PAGE and immunoblotting. An

protein expression by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) according to the method of Laemmili (1970) , with slight modifications. Samples were solubilized in SDS-PAGE sample buffer containing 75 m m Tris–HCl (Ph, 6.8), 50

dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer [60 m m Tris-HCl (pH 6.8), 25% glycerol (v/v), 2% SDS (w/v), 5% b-mercaptoethanol (v/v), 0.1% bromophenol blue (w/v)] with a ratio of 4:1, heated at 95 °C for 5 min, and then

supernatant were loaded and separated by 10% SDS–polyacrylamide gel electrophoresis and then transferred to the polyvinyl diisorpropyl fluoride transfer membrane (Perkin Elmer, Waltham, MA). The membrane was incubated with the generated polyclonal antiserum

extracted from the transformed E. coli and control cell lines when the o.d. 600 reached 0.6. Equal amounts of protein (30 µg per sample) were resolved on a 17% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel and stained using

individuals in a population may enhance the reliability of our results. Amplified products that showed a band of the expected size were separated on 8% denaturing polyacrylamide gel electrophoresis and visualized by silver staining. The primers that were not

( C. sativus ) grafted on figleaf gourd ( C. ficifolia ) or on pumpkin ( C. maxima ). They found four proteins on sodium dodecylsulfate polyacrylamide gel electrophoresis gels in grafted scions that do not appear in control plants but matched the

. Reactions were completed by incubating at 72 °C for 8 min. The amplified products were resolved on 6% polyacrylamide gel electrophoresis containing 7 m urea and detected by an automated sequencer (ALFexpress II DNA Analysis System; GE Healthcare). Alleles