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Tao Wu and Jiashu Cao

at 470 nm after starting the reaction with addition of H 2 O 2 . The specific activity of peroxidase was expressed as units per milligram of protein per minute (U·mg −1 ·min −1 ). Native polyacrylamide gel electrophoresis (PAGE) and isozyme

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Alessandra Ghiani, Noemi Negrini, Silvia Morgutti, Federica Baldin, Fabio F. Nocito, Anna Spinardi, Ilaria Mignani, Daniele Bassi, and Maurizio Cocucci

state for non-denaturing polyacrylamide gel electrophoresis (PAGE) with evaluation of in gel PG activity or desalted with a Plus One 2-D Clean-Up Kit (GE Healthcare Europe, Milan, Italy) for sodium dodecyl sulfate (SDS)-PAGE. Protein content was

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Mahalaxmi Veerasamy, Yali He, and Bingru Huang

protein expression by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) according to the method of Laemmili (1970) , with slight modifications. Samples were solubilized in SDS-PAGE sample buffer containing 75 m m Tris–HCl (Ph, 6.8), 50

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Kemin Su, Justin Q. Moss, Guolong Zhang, Dennis L. Martin, and Yanqi Wu

595 nm in a spectrophotometer (Novaspec Plus; Amersham BioSciences, Piscataway, NJ). The remaining supernatant was retained for sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. SDS-PAGE and immunoblotting. An

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Hanseul Park and Yeh-Jin Ahn

extracted from the transformed E. coli and control cell lines when the o.d. 600 reached 0.6. Equal amounts of protein (30 µg per sample) were resolved on a 17% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel and stained using

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Min Fan, Yike Gao, Yaohui Gao, Zhiping Wu, Hua Liu, and Qixiang Zhang

individuals in a population may enhance the reliability of our results. Amplified products that showed a band of the expected size were separated on 8% denaturing polyacrylamide gel electrophoresis and visualized by silver staining. The primers that were not

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Zhong-Bin Wu, Hsin-Mei Ku, Yuh-Kun Chen, Chung-Jan Chang, and Fuh-Jyh Jan

supernatant were loaded and separated by 10% SDS–polyacrylamide gel electrophoresis and then transferred to the polyvinyl diisorpropyl fluoride transfer membrane (Perkin Elmer, Waltham, MA). The membrane was incubated with the generated polyclonal antiserum

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Tao Hu, Haiying Yi, Longxing Hu, and Jinmin Fu

dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) sample buffer [60 m m Tris-HCl (pH 6.8), 25% glycerol (v/v), 2% SDS (w/v), 5% b-mercaptoethanol (v/v), 0.1% bromophenol blue (w/v)] with a ratio of 4:1, heated at 95 °C for 5 min, and then

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Jana Murovec, Natasa Stajner, Jernej Jakse, and Branka Javornik

. Reactions were completed by incubating at 72 °C for 8 min. The amplified products were resolved on 6% polyacrylamide gel electrophoresis containing 7 m urea and detected by an automated sequencer (ALFexpress II DNA Analysis System; GE Healthcare). Alleles

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Jianming Sun, Yiming Liu, Xianglin Li, and Bingru Huang

SDS–polyacrylamide gel electrophoresis, gels were washed in deionized distilled H 2 O three times for 15 min and stained with colloidal Coomassie Blue G-250 ( Neuhoff et al., 1988 ). The 2-DE gels were scanned using Image Scanner (Amersham Biosciences