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Hussam S.M. Khierallah, Saleh M. Bader, Michael Baum, and Alladin Hamwieh

frequency ranged from 0.140 to 0.909 for PDCAT18 and mPdCIR057, respectively, with an average of 0.436. High levels of heterozygosity were detected in female cultivars with an average of 0.503 ( Table 3 ). Genetic distance among cultivars varied from 0

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Hongwen Huang, Desmond R. Layne, and Don E. Riemenschneider

As a new National Clonal Germplasm Repository for Asimina species at Kentucky State University (KSU), of major concern to us is the genetic variation within our germplasm collection. The present study investigated the extent of genetic diversity for the pawpaw germplasm in our collection and the geographical pattern of genetic diversity among populations using isozyme markers. Allozyme diversity was high in Asimina triloba (L.) Dunal (Annonaceae) collected from all nine different states, as is typical for temperate woody perennial, widespread and outcrossing plant species. Averaged across populations, mean number of alleles per locus (A), percent polymorphic loci (P), effective number of alleles per locus (Ae), and expected heterozygosity (He) were 1.54, 43.5, 1.209, and 0.172, respectively. Significant deviations from Hardy-Weinberg equilibrium were found in nine populations at an average of 4.8 loci. Observed heterozygosity was higher than expected. Partitioning of genetic diversity showed that 88.2% resided within populations. The proportion of genetic diversity among populations (Gst = 0.118; FST = 0.085) was either lower than or within the range of those species with similar ecological and life-history traits. The mean genetic identity among populations was high (I = 0.988). An analysis using UPGMA clustered most populations as one major group, with the southernmost (Georgia) and the westernmost (Illinois) populations readily separated from the main group. The relationships discovered by principal component analysis (PCA) were similar to those revealed by UPGMA. In addition, PCA separated the northernmost population (New York) from the major group. Sampling strategies for future germplasm collection of A. triloba are also discussed.

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James Nienhuis, Mary K. Slocum, Dawn A. DeVos, and Roger Muren

Genetic similarities were calculated among 89 Brassica oleracea L. genotypes, which included 62 broccolis (var. italica), 16 cauliflowers (var. botrytis), and 11 cabbages (var. capitata). These entries represented a wide range of commercially available germplasm, including open-pollinated cultivars, commercial hybrids, the inbred parents of several hybrid cultivars, and 27 entries that were provided as unknowns. Sixteen random genomic clones were used as probes in Southern hybridizations to detect restriction fragment length polymorphism (RFLP). From each of the random probes, an average of four polymorphic bands were classified as to their presence or absence for each genotype. The genetic similarity between ail pairs of genotypes was calculated. A multidimensional scaling (MDS) plot indicated that the broccoli, cauliflower, and cabbage groups were clustered with very little overlap. Within groups, genetic similarity corresponded to relationships based on available pedigree information. Comparison of banding patterns between hypothetical and actual hybrids was used to correctly identify the parents of several parent-hybrid combinations. The RFLP pattern of a hybrid and one of the parents (female) were used to predict the genotype and identity of the other parent (male).

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Yiqun Weng

coefficients ( Jaccard, 1901 ) to estimate the genetic diversity among the C. metuliferu s accessions and between these and the outgroup members. Genetic distance estimates were calculated as the complement of Jaccard's similarity coefficient ( Spooner et al

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Darren H. Touchell, Thomas G. Ranney, Dilip R. Panthee, Ronald J. Gehl, and Alexander Krings

relationships between the subgroups within each species are reflected by the genetic distant coeffecients ( Table 4 ). In all cases, the within-group genetic distance was considerably lower than the among-group genetic distance and provided further support for

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Amanda J. Hershberger, Tracie M. Jenkins, and Carol Robacker

,000 simulations. A matrix of Nei’s unbiased genetic distances ( Nei, 1978 ) was calculated with PopGene Version 1.32 using all markers and used to construct an unrooted unweighted pair group method with arithmetic mean (UPGMA) phenogram with PHYLIP Version 3

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Cunquan Yuan, Zhiyi Qu, Huitang Pan, Tangren Cheng, Jia Wang, and Qixiang Zhang

that the genes that make up S -loci, such as P and A, may have multiple alleles. They also determined the genetic distance between G, P, and A. Li et al. (2008) found marker PvGlo linked to the S -locus and with a function similar to those

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Fuad Gasi, Silvio Simon, Naris Pojskic, Mirsad Kurtovic, Ivan Pejic, Mekjell Meland, and Clive Kaiser

, 1984 ). Analyses of molecular variance ( Exoffier et al., 1992 ), based on the stepwise mutation model ( Ohta and Kimura, 1973 ), was performed using GenoType software with 1000 permutations. Genetic distance between accessions ( Bruvo et al., 2004

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Cristina Zambrana-Echevarría, Lorriane De Jesús-Kim, Rocio Márquez-Karry, Dimuth Siritunga, and David Jenkins

Australian isolates. American and Caribbean isolates showed distances of 0.07–0.08 at the nucleotide level and 0.09–0.10 at amino acid to PR-PRSV. Asian isolates have less similarity and more genetic distance to the Puerto Rican isolates, where the isolate

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Mengmeng Zhang, He Huang, Qing Wang, and Silan Dai

shown in Supplemental Fig. 1 ) were selected as crossing parents from 37 superior genotypes, and six hybridized combinations were matched using them ( Table 6 ) according to the following principles: genetic distance was farther between crossing parents