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Geoffrey Meru and Cecilia E. McGregor

the parents, the F 1 and each of the F 2 plants using the E.N.Z.A 96-well format kit (Omega Bio-tek, Norcross, GA) according to the manufacturer’s instructions. The concentration and quality of the DNA was determined by absorbance measurements

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Yinping Li, Raymond A. Cloyd, and Nora M. Bello

, Oakland, CA) with a round hole (9.5 cm diameter) cut in the lid that was covered with No-Thrips insect screening (mesh size: 0.15 × 0.15 mm) (Green-Tek, Janesville, WI). Green beans ( Phaseolus vulgaris L.) were purchased from a local supermarket (Dillons

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Yuya Mochizuki, Saori Sekiguchi, Naomi Horiuchi, Thanda Aung, and Isao Ogiwara

), green (λ = 525 nm), and red (λ = 625 nm) LEDs (OKI Digital Imaging Co., Ltd., Gunma, Japan). The wavelengths were measured by MK 350 (JPR tek, Mial-Li country 350, Taiwan) ( Fig. 1 ). Photosynthetic photon flux density ( PPFD ), Pn, g S , Tr, and Ci

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Haiyan Wang, Ran Chen, Yuefan Sheng, Weitao Jiang, Rong Zhang, Xuesen Chen, Xiang Shen, Chengmiao Yin, and Zhiquan Mao

= 0.993, y = −2.291x + 36.396; Standard curve of F. solani : R 2 = 0.994, y = −2.352x + 26.941) The E.Z.N.A. soil DNA extraction kit (Omega Bio-Tek, Norcross, GA) was used to extract DNA. The primers for fungi were ITS1 (F: 5'-AACCTGCGGAAGGATCATT-3

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Mary Vargo and James E. Faust

flux density ( PPFD ) (Tempa Climate Screen; Ludwig Svensson, Charlotte, NC), while one-third of the sections were shaded with 30% PPFD -reduction shadecloth (Green-Tek Inc., Dinuba, CA). The remaining one-third of the sections received the ambient

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Nicolas C. Strange, John K. Moulton, Ernest C. Bernard, William E. Klingeman III, Blair J. Sampson, and Robert N. Trigiano

DNA Kit (Omega Bio-Tek, Norcross, GA). For large specimens (e.g., Bombus spp. and Svastra spp.), one leg was used; for smaller taxa (e.g., members of the tribe Augochlorini ), the three left legs were used. For very small insects [e

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Devin L. Radosevich, Raymond A. Cloyd, and Nathan J. Herrick

.4 × 9.4 cm (length × width × height)] with No-Thrips insect screening (mesh = 150 × 150 μm: Green-Tek Inc., Janesville, WI) at 24 to 27 °C, 50% to 60% relative humidity, and constant light in a laboratory of the Department of Entomology at Kansas State

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Rachelyn C. Dobson, Mary Rogers, Jennifer L.C. Moore, and Ricardo T. Bessin

1/25-inch fine mesh, 30% woven shadecloth (Green.Tek, Janesville, WI). At the Kentucky site in 2013, black 1/25-inch mesh screens (Dewitt, Sikeston, MO) were tested in place of the white screens, as available quantities of the white 1/25-inch mesh

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Manjul Dutt, Daniel Stanton, and Jude W. Grosser

pieces for longitudinal sections and 2.5-mm pieces for cross-sections) and processed for microscopy. Stem pieces were arranged and mounted in Tissue Tek Cryo-OCT Compound (Thermo-Fisher Scientific, Waltham, MA) on a brass stub. The stubs were frozen and

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Bizhen Hu, Mark A. Bennett, and Matthew D. Kleinhenz

room and constructed using a polyvinyl chloride frame covered by single layers of clear plastic sheeting and black knitted shadecloth (50% photosynthetic active radiation transmission; Tek, Janesville, WI). Moisture was maintained using the same type of