The aim of this work was to study different apple of somatic material as callus and adventitious shoots are concerned, for further utilization in the research of somaclonal variation. The somatic materials were: leaf discs, cotyledons and hypocotyls of Gala apple seedlings, cultivated in a MS medium added by B5 vitamins in addition to (in mg/l): BAP (1,0), NAA (0,5) mio-inositol (100,0) sucrose (30,0 g/l) and solidified in agar (6,0 g/l). The several times of explant exposition to the dark affected the final callus weight. Callus weight derived from leaf discs were higher than those for cotyledons and hypocotyls. Explants exposed directly under light or up to two weeks in the dark showed less percentage of regenerative callus as compared to those of three weeks in the dark. The leaf explants presented the highest percentage of regenerative callus. The least response was obtained for those derived from hypocotyls. The highest number of adventitious shoots was obtained keeping the explants three weeks in the dark as compared to directed light exposition.
Gerson R. de L. Fortes and Silvio L. Teixeira
Raquel Gomez and Lee Kalcsits
‘Honeycrisp’ is among the most widely grown apple cultivars in the United States and ‘WA 38’ is a new apple cultivar released in Washington State. ‘Honeycrisp’ is highly susceptible to bitter pit and other physiological disorders; however, ‘WA 38’ is not susceptible to bitter pit but little is known about its susceptibility to other disorders. Bitter pit is a calcium-related disorder that has been associated with localized calcium deficiencies in fruit in addition to the proportions of calcium relative to the presence of other nutrients like potassium and magnesium. The objective of this study was to compare physiological differences and fruit quality between ‘Honeycrisp’ and ‘WA 38’ to determine how these differences might correspond to differences in mineral nutrient composition and bitter pit susceptibility. Here, ‘Honeycrisp’ and ‘WA 38’ elemental composition in leaves, fruit, and xylem sap was measured every 20 days starting 30 days after full bloom and compared with leaf gas exchange and stem water potential. ‘Honeycrisp’ had greater foliar transpiration rates that corresponded with greater calcium in the leaves and lower leaf K+Mg/Ca ratio, when compared with ‘WA 38’. In contrast, fruit calcium concentrations were higher for ‘WA 38’ with lower K+Mg/Ca ratios. Xylem conductance was higher during late summer in ‘WA 38’ compared with ‘Honeycrisp’. ‘WA 38’ fruit was denser than ‘Honeycrisp’ and more research is needed to determine whether differences in fruit structure may affect susceptibility to bitter pit in apple.
F.F. Ahmed, A.M. Akl, A.A. Gobara, and A.E.M. Monsour
The beneficial effect on yield and quality of `Anna' apple fruits for the application of ascobine at 0.1% and citrine at 0.6% was studied during 1995 and 1996. Results showed that two citrine sprays at start of growth and 30 days later of ascobine at 0.1% or citrine at 0.6% were of material promotion effect on yield, fruit weight, total soluble solids, and total sugars, while reducing the total acidity. Both fertilizers were equally very effective in all the studied characters. The most striking and promising treatment was the application of ascobine at 0.1% or citrine at 0.6% twice during the growing season; i.e., growth start at 30 days later.
D.C. Ferree, J.C. Schmid, and B.L. Bishop
Survival of replicated rootstock plantings of apple trees (Malus ×domestica) to fire blight (Erwinia amylovora) infection shows that a wide range of rootstock susceptibility exists. Trees on `Malling 26' (M.26), `Malling 9' (M.9), and `Mark' consistently had significant losses. Of the dwarfing rootstocks widely available commercially, `Budagovsky 9' (B.9) survived well with productive trees, but was not resistant to fire blight infection. The following experimental rootstocks had good survivability with many live productive trees in one or more trials: `Poland 2' (P.2), `Vineland 1' (V.1), `Malling 27 EMLA' (M.27 EMLA), `Budagovsky 491' (B.491), `Budagovsky 409' (B.409), `Vineland 7' (V.7), `Vineland 4' (V.4), and `Oregon Rootstock 1' (OAR1).
James D. Hansen, Dennis J. Albano, and Millie L. Heidt
The two-component quarantine treatment was shown to be effective against at least 7,000 codling moth (Cydia pomonella) fifth instar larvae infesting `Fuji' apples (Malus × domestica) in each required confirmation test involving two sizes of cartons. After cold storage for 55 days at 36 °F (2.2 °C), infested fruit were placed in vented cartons, either 20-lb [7 × 12 × 12.5 inches (17.8 × 30.5 × 31.8 cm)], or 40-lb [12 × 12.5 × 20.5 inches (30.5 × 31.8 × 52.1 cm)], then fumigated with 0.056 oz/ft3 (56 g·m-3) of methyl bromide for 2 hours at 50 °F (10.0 °C). After each treatment, either no survivors were present or no moribund larvae survived beyond the first week of post evaluation of the larvae.
Peter M.A. Toivonen and Pascal Delaquis
Use of sprays to sanitize and treat apple (Malus ×domestica) slices helps to reduce the potential for cross-contamination that can occur when treatments are done in dip tanks. This research examined several factors that may affect the efficacy of spray treatments: 1) spray volume; 2) efficacy of spray application of anti-browning solution (ABS) compared with dipping; 3) effect of slice density during spraying; and 4) effect of the addition of an antimicrobial compound, vanillin, on microbiologically associated browning. Low-volume sprays (36-50 mL·kg-1 slices) of ABS gave maximal control of browning and this was equivalent to the control afforded by a 2-minute dip in the ABS. Spray application resulted in significant reduction in incidence and severity of microbiologically associated “secondary browning” as compared with dip application. However, if more than one layer of slices were present on the support mesh during the spray treatment, then secondary browning increased. This was attributed to potential cross-contamination between layers of apples in the spray treatment. Addition of vanillin into the ABS resulted in a 50% reduction of the incidence of “secondary browning.” Low-volume spray applications of ABS can be managed such that the microbiologically associated “secondary browning” is much lower than possible with dip application.
Michael A. Arnold and Eric Young
Bare-root Malus × domestica Borkh. seedlings were chilled for 0, 600, 1200, or 1800 hours at 5C (CH). Seedlings were then placed with roots and/or shoots in all combinations of 5 and 20C forcing conditions (FC) for up to 21 days. Virtually no growth occurred at 5C FC. When the whole plant was forced at 20C, all measures of root and shoot growth increased in magnitude, occurred earlier and at a faster rate with increasing CH. Thus, roots and shoots responded similarly to chilling. When shoots or roots were subjected to 20C FC, while the other portion of the plant was at 5C, the responses were reduced in magnitude and delayed. However, the overall growth enhancement by chilling was not negated. Root and shoot growth enhancement by chilling appeared to be increased if the other portion of the plant was actively growing also, but not dependent on it. Growth of adventitious shoots on roots (root suckers) was greatly enhanced with increasing CH on plants subjected to 5C shoot and 20C root FC. While total root and shoot bark protein levels on a per-seedling basis were similar, protein concentrations were lower in root bark than in shoot bark. During chilling, total protein per seedling generally increased until just before the time that chilling requirements for vegetative budbreak were satisfied. Protein degradation then began, resulting in lower protein levels through 2300 CH. Rapid protein breakdown (1200 to 1800 CH, roots; 1000 to 1800 CH, shoots) occurred at about the same time that root (1000 to 1800 CH) and shoot (800 to 1800 CH) growth responses to chilling were increasing. Warm FC resulted in increased protein breakdown with increased CH and forcing time.
S.R. Drake, D.C. Elfving, M.A. Drake, T.A. Eisele, S.L. Drake, and D.B. Visser
This study was conducted over two crop seasons using `Scarletspur Delicious' and `Gale Gala' apple trees (Malus ×domestica). The bioregulators aminoethoxyvinylglycine (AVG), ethephon (ETH), and 1-methylcyclopropene (MCP) were applied at various times before or after harvest. Fruit response was evaluated at harvest and after regular atmosphere (RA) and controlled atmosphere (CA) storage [2.0% oxygen (O2) and <2.0% carbon dioxide (CO2) at 0 °C] and quality of whole and juice apple products evaluated. AVG reduced starch loss and ethylene production, enhanced firmness, and reduced cracking in `Gale Gala,' but reduced sensory acceptance of apples and apple juice. ETH intensified starch loss, ethylene production, and reduced firmness, but did not affect `Gale Gala' fruit cracking. AVG followed by ETH reduced starch loss, ethylene production, and cracking and maintained firmness. This combination also aided in sensory acceptance of apples but reduced sensory preference of apple juice. Exposure to postharvest MCP improved flesh firmness retention and reduced ethylene production after both RA and CA storage. MCP either favored or reduced sensory acceptance of whole apples, depending on the particular season, but reduced sensory preference of apple juice. Sensory scores for `Scarletspur Delicious' apples were more strongly modified by bioregulators than were `Gale Gala' apples.
Renae E. Moran
The goal of this project was to evaluate the effectiveness of aminoethoxyvinylglycine (AVG) for increasing effectiveness of 1-methylcyclopropene (1-MCP) for maintaining firmness and preventing scald in `McIntosh' and `Cortland' apples (Malus ×domestica). AVG and 1-MCP used together maintained `McIntosh' apple firmness more than 1-MCP used alone after 120 or 200 days of controlled-atmosphere (CA) storage. AVG and 1-MCP can be used to maintain firmness of `McIntosh' when internal ethylene concentration (IEC) at harvest is as high as 240 μL·L-1, but CA storage life is limited to 4 months. AVG was not effective at increasing efficacy of 1-MCP on `Cortland' when IEC at harvest was not significantly different between AVG-treated and untreated fruit and IEC was less than 2 μL·L-1. AVG increased efficacy of 1-MCP on `Cortland' when IEC was 36 μL·L-1 in untreated fruit compared to undetectable in AVG treated fruit. 1-MCP prevented scald of `Cortland' in 1 year and reduced it to 5% or less in another year when fruit were stored 120 days. 1-MCP reduced `Cortland' scald to 34% or less after 200 days of storage.
Richard P. Marini
The relationship between fruit mass and fruit diameter for apple fruit 2 to 26 mm (0.08 to 1.04 inches) in diameter was evaluated for two cultivars sampled from several orchards for three years. A single regression model was appropriate for all combinations of year, cultivar, and orchard. The model was used to develop a chart to quickly estimate fruit diameter from fruit mass. These fruit diameter estimates can be used by commercial fruit growers to time chemical thinner sprays.