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DNA polymerase in PCR with final concentrations of 4.5 m m of MgCl 2 , 0.2 m m of dNTPs, 0.2 μ m of the adequate primers, and 0.75 U of Dream Taq DNA polymerase (Fermentas). Table 2. Simple sequence repeat loci developed in different Prunus

abundance, enormous extent of allelic diversity, and the ease of assessing simple sequence repeat (SSR) size variation by PCR with pairs of flanking primers ( Agarwal et al., 2008 ; Muzzalupo et al., 2006 , 2008 ; Rekik et al., 2008 ). Recently, many

microsatellite markers [simple sequence repeats (SSRs)] ( Baldoni et al., 2009 ; La Mantia et al., 2005 ). The microsatellite technique is one of the most reliable methods used in olive cultivar characterization. It has revealed a high informative level

.F. Boritzki, M. Struss, D. 2001 DNA fingerprinting of tetraploid cherry germplasm using simple sequence repeats J. Amer. Soc. Hort. Sci. 126 205 209 Contreras, R.N. Ranney, T.G. 2007 Reproductive

.J. Pineda, A. Cosson, P. Dirlewanger, E. Ascasibar, J. Cipriani, G. Ryder, C. Testolin, R. Abbott, A. King, G. Iezzoni, A. Arús, P. 2003 A set of simple-sequence repeat

-five polymorphic genic simple sequence repeats (SSRs) applied for analysis of 53 Chinese cymbidium accessions. Evaluation of marker discriminatory power and construction of panels. D scores were used to measure the ability of markers for correct assignment of

. 2002 Identification of simple sequence repeats (SSRs) in olive ( Olea europaea L.) Theor. Appl. Genet. 104 301 307 Charafi, J. El Meziane, A. Moukhli, A. Boulouha, B. El Modafar, C. Khadari, B. 2008 Menara gardens: A Moroccan olive germplasm collection

genetic differentiation among the different materials. Table 2. Genetic diversity analysis of the 100 Vitis accessions by the nine simple sequence repeat markers. According to the cluster analysis, 100 grape samples could be divided into five groups. The

among the accessions. Fruits of ‘Hongyang’ and L exhibited the highest sugar/acid ratio ( Table 3 ). Simple sequence repeat marker confirmation and principal component analysis. The number of alleles per locus of 12 polymorphic expressed sequence tag

peaks (Applied Biosystems). Table 2. Description of the simple sequence repeat loci used in the analysis of 93 Lagerstroemia cultivars and interspecific hybrids, including repeat motifs, primer sequences, and statistical analyses of results. Data