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Tripti Vashisth and Taylor Livingston

study are presented in Table 1 . Primers were designed either manually or using PrimerQuest (Integrated DNA Technologies, Coralville, IA). All qRT-PCR reactions were performed using a real-time PCR system (Fast 7500 cycler; Applied Biosystems, Foster

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John R. Stommel and Judith M. Dumm

spectrophotometer (ND1000; NanoDrop Technologies, Wilmington, DE) and integrity and quantification confirmed using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Real-time PCR was used to compare flavonoid gene expression ( Chs , Dfr , Ans , Myb, Myc

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Kaori Ando and Rebecca Grumet

real-time polymerase chain reaction (qRT-PCR) assays indicate high reproducibility and suitability for gene expression analysis ( Ohtsu et al., 2007 ; Torres et al., 2008 ; Weber et al., 2007 ). In this study, we used 454-pyrosequencing to

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Seung Hee Eom and Tae Kyung Hyun

(NT). Supplemental Fig. 3. Transcript levels of BraHATs in different tissues. Data are means ± SE. Supplemental Table 1. Primer sequences for quantitative real time polymerase chain reaction analysis.

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Yanjie Wang, Yeqing Chen, Man Yuan, Zeyun Xue, Qijiang Jin, and Yingchun Xu

. Expression levels of seven flavonoid biosynthetic structural genes were analyzed using real-time quantitative PCR (qRT-PCR) on a Mastercycler ep realplex instrument (Eppendorf, Hamburg, Germany). The specific primer sets for individual genes used for qRT-PCR

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Jingyi Lv, Yonghong Ge, Canying Li, Mengyuan Zhang, and Jianrong Li

with an ultraviolet transilluminator (Uvitec FireReader; Uvitec, Cambridge, UK). Real-time quantitative PCR analysis. The RNA concentration from each weekly sample was measured using a spectrophotometer (NanoDrop ND-1000; Thermo Fisher Scientific

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Julio Solis, Arthur Villordon, Niranjan Baisakh, Don LaBonte, and Nurit Firon

transplanting) vs. control by quantitative real-time polymerase chain reaction. Error bars represent sem of two independent experiments averaged over three replications each except IbBEL2 , Ibkn2 , and IbCRF2 , where the se was calculated over the mean of

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Yuanyuan Miao, Qiaosheng Guo, Zaibiao Zhu, Xiaohua Yang, Changlin Wang, Yuan Sun, and Li Liu

analysis by qRT-PCR. To assay the expression levels of mRNA of putative key genes associated with stolon formation in T. edulis , qRT-PCR was performed using ABI 7300 Real-Time PCR System (Applied Biosystems, Foster City, CA) with fluorescence dye SYBR

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Xuhong Zhou, Xijun Mo, Yalian Jiang, Hao Zhang, Rongpei Yu, Lihua Wang, Jihua Wang, and Suping Qu

). Real time polymerase chain reaction (RT-PCR) was carried out on 50 ng of cDNA with the OSDL1a-422F and OSDL1a-656R primers for OSDLa , the OSDL1b-99-F, and OSDL1b-223-R primers for OSDLb , and the OSDL1c-133F and OSDL1c-270-R primers for OSDLc

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Xinyi Chang, Junli Sun, Lianling Liu, Wang He, and Baolong Zhao

performed, which indicated that the DEGs were mainly involved in photosynthesis and porphyrin and chlorophyll metabolism. Quantitative real-time-PCR (qPCR) analysis. To verify the reliability of the photosynthetic transcriptome data of jujube seedlings under