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A.J. Both, E. Reiss, J.F. Sudal, K.E. Holmstrom, C.A. Wyenandt, W.L. Kline, and S.A. Garrison

film [6 mil thick (model GT IR/AC; Green.Tek, Edgerton, WI)]. Additional information about high tunnel construction and operation were described in Lamont et al. (2002) , Lamont and Orzolek (2003) , Jett et al. (2004) , and Reiss et al. (2004) . In

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Ulrike C.M. Anhalt, Katharina Martini, Ernst-Heinrich Ruehl, and Astrid Forneck

Kit (Omega Bio-Tek, Norcross, GA) following the manufacturer’s instructions with one modification, prolonging the incubation step to 30 min. The cultivar collection was mainly obtained as extracted DNA from the Geisenheim Research Centre, University of

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W. Robert Trentham, Carl E. Sams, and William S. Conway

(Tissue-Tek ® ; Electron Microscopy Sciences, Hatfield, PA). Sections 10 μm thick were cut with high-profile disposable blades on a rotary microtome. Ribbons of the sections were divided into segments, flattened on a water bath at 45 °C, and pulled off

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Shiow Y. Wang, Kim S. Lewers, Linda Bowman, and Min Ding

dilution. The ORAC assay was carried out using a high-throughput instrument platform consisting of a robotic eight-channel liquid handling system (Precision 2000; Bio-Tek Instrument, Winooski, VT) and a microplate fluorescence reader (FL ×800; Bio-Tek

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Gyeong Ran Do, Ju Hee Rhee, Wan Soon Kim, Yun Im Kang, In Myung Choi, Jeom Hwa Han, Hyun Hee Han, Su Hyun Ryu, and Han Chan Lee

appears disorganized and translucent due to the poorly resolved vacuole ( Vizcay Barrena and Wilson, 2006 ). TRANSPOSABLE ELEMENT SILENCING VIA AT-HOOK (TEK) mutant leads to male sterility caused by the absence of exine and intine layers ( Lou et al., 2014

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Sabin Khanal, Sarah R. Hind, and Mohammad Babadoost

′-TCGCCCAGCGTCATCAGGCCATC-3′), which amplify a 420 bp fragment of hrp B7 ( Obradovic et al., 2004 ), were used for PCR amplification. For each isolate, a 25-μL mixture containing Taq DNA polymerase (Omega Bio-Tek Inc., Norcross, GA), RST 65/69 primers

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Qiannan Hu, Fei Ding, Mingna Li, Xiaxiang Zhang, Shuoxin Zhang, and Bingru Huang

-transcriptase polymerase chain reaction (qRT-PCR). Total RNA was extracted from leaf tissue with E.Z.N.A. Plant RNA Kit (Omega Bio-tek, Norcross, GA), and contaminating genomic DNA was removed with TURBO DNA-free reagent (Life Technologies, Grand Island, NY). Next, 2 μg of

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Nathan J. Herrick and Raymond A. Cloyd

two wire mesh greenhouse benches (4.3 × 1.1 m). Each cage had a lid with a hole on top and two additional holes on opposite sides (12.7 cm diameter) covered with thrips screening (Green-Tek, Inc., Edgerton, WI). The screened holes allowed for

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Juan Carlos Díaz-Pérez, Kelly St. John, Mohammad Yamin Kabir, J. Alberto Alvarado-Chávez, Ania M. Cutiño-Jiménez, Jesús Bautista, Gunawati Gunawan, and Savithri U. Nambeesan

(41% shade) ( Fig. 1 ). Shade factors are as reported by the manufacturer (Green-tek, Janesville, WI) and are within the optimal shade level (30% to 46% shade) for bell pepper ( Diaz-Pérez, 2014 ). Nets were placed on the top of wooden rectangular

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Qin Shi, Yunlong Yin, Zhiquan Wang, Wencai Fan, Jinbo Guo, and Jianfeng Hua

further purification by an ELISA (ELISA; Jiancheng Institute of Biology and Engineering, Nanjing, China) using a monoclonal antibody. Absorbance values were recorded by a microplate reader (Bio-Tek; Winooski, VT) at 450 nm. The content of H 2 O 2 was