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polymorphic loci (SAMPL) ( Tseng et al., 2002 ), and simple sequence repeat markers (SSRs) ( Gichuru et al., 2006 ). Microsatellites developed for genotyping sweetpotato ( Buteler et al., 1999 ; Hu et al., 2004 ) have been widely used for diversity analysis

extraction and simple sequence repeat generation. DNA was extracted from roots or shoots of plants collected as propagules, which were collected from the field or golf course and moved to a glasshouse to accumulate tissue. For extraction of DNA from shoots

subgenus Lithocerasus , and 22 accessions (seven species and two hybrids) to subgenus Cerasus ( Table 1 ). Table 1. Forty Prunus accessions used in genetic relationship analysis among 10 rootstock species from China by simple sequence repeat. Genomic

one-, two-, or three-base tag attached to the 3′ or 5′ end of the repeat. The tag assures that the primer anneals to the end of the simple sequence repeat and thus provides greater specificity. The benefit of using ISSR PCR over other methods include

and genes responsible for the morphological characteristics of plants is necessary for the molecular breeding of R. idaeus . Simple sequence repeats are tandem repeats of 1–6-bp sequence motifs. They are considered the most suitable because of their

marker system to use for a particular application depends on its ease of use and the particular objectives of the investigation ( Rafalski et al., 1996 ). Recently, inter-simple sequence repeat (ISSR) markers have emerged as an alternative system with the

additional four cultivars from the University of Georgia ( Table 1 ). Table 1. The origins of 58 Paspalum vaginatum accessions and four cultivars used in genetic diversity analysis revealed with simple sequence repeat markers. All materials were propagated

produced between those rootstocks bearing different genetic backgrounds. Fig. 1. Simple sequence repeat band pattern of the 41 apple rootstocks generated by the primer pair CH04d02. Lanes 1 to 49: (1) ‘Pingyitiancha', (2) ‘Xiaojinhaitang', (3) ‘Y.d002', (4

collected in Taiwan and appear to be the only H. paniculata germplasm in the United States that was not either introduced from Japan or bred from Japanese germplasm. Microsatellite, or simple-sequence repeat (SSR), markers provide a useful method for

germplasm collection of USDA-ARS at Byron, GA, used to test genetic relationships based of simple sequence repeat markers. Table 2. Accessions from related Prunus species and hybrids tested for cross-transferability of peach simple sequence repeats. z Leaf