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Fatemeh Khodadadi, Masoud Tohidfar, Mehdi Mohayeji, Abhaya M. Dandekar, Charles A. Leslie, Daniel A. Kluepfel, Timothy Butterfield, and Kourosh Vahdati

were aligned with walnut genome sequences (A.M. Dandekar, unpublished data), using BLAST to identify the walnut version of the tomato P14a . Real-time PCR. Three replicates of whole leaf samples detached at 0, 24, 72, 96, 120, and 144 h after

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Yan Hong and SiLan Dai

by RT-qPCR after 40 cycles of amplification. The specificity of the primer amplicons was verified by 2% (w/v) gel electrophoresis. RT-qPCR analysis. PCR reactions were performed using a Mini Opticon Real-time PCR System (Bio-Rad, Hercules, CA) based

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Lin Zhou, Qianqian Shi, Yan Wang, Kui Li, Baoqiang Zheng, and Kun Miao

quantitative real-time polymerase chain reaction (qRT-PCR) in tree peony. Primers were designed using the Primer Premier 5.0 software (Premier Biosoft International, Palo Alto, CA) with the following parameters: melting temperatures 60 to 65 °C, primer lengths

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Rhiana F. Jones, Paul W. Bosland, Robert L. Steiner, Richard W. Jones, and Mary A. O’Connell

-glucanases Phytochemistry 71 142 148 Sellars, M.J. Vuocolo, T. Leeton, L.A. Coman, G.J. Degnan, B.M. Preston, N.P. 2007 Real-time RT-PCR quantification of Juruma shrimp transcripts: A comparison of relative and absolute quantification procedures J. Biotechnol. 129 391 399

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Jitao Li, Nian Wang, Lina Wang, Haiping Xin, and Shaohua Li

sequences was constructed using the neighbor-joining (NJ) method of the MEGA software [Version 4.0 ( Tamura et al., 2007 )]. Real-time PCR analysis. For the expression analysis, 1-month-old tissue culture seedlings, fully adapted to growing in a growth

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Harpartap S. Mann, Jennifer J. Alton, SooHee Kim, and Cindy B.S. Tong

sequence similarity search algorithms were used to find significant sequence similarities to annotated genes in online genomic databases. Quantitative real-time PCR (qRT-PCR). Using cDNA as template, relative transcript levels of genes for four cell

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Huiling Wang, Wei Wang, Weidong Huang, and Haiying Xu

/g FW. Three samples were drawn from each replication and measurements were taken for each sample. RNA preparation, RT-PCR, and quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Total RNA was isolated from cells using the CTAB method

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Lisa A. Beirn, William A. Meyer, Bruce B. Clarke, and Jo Anne Crouch

the course of the experiments. Inoculated plants were monitored until rust pustules developed. Spore morphology and real-time PCR. Upon uredinia development, spores were harvested by hand to confirm species identity. Morphological evaluations to

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Concetta Licciardello, Biagio Torrisi, Maria Allegra, Fabiola Sciacca, Giancarlo Roccuzzo, Francesco Intrigliolo, Giuseppe Reforgiato Recupero, Paola Tononi, Massimo Delledonne, and Vera Muccilli

staining, respectively. RNA samples were used for real-time RT-PCR experiments. Microarray analysis. Expression analysis was performed on a custom 90K microarray (CombiMatrix; CustomArray, Bothell, WA), which contained 7697 specific probes 35 to 40 bp in

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Tingting Zhao, Jingkang Hu, Yingmei Gao, Ziyu Wang, Yufang Bao, Xiaochun Zhang, Huanhuan Yang, Dongye Zhang, Jingbin Jiang, He Zhang, Jingfu Li, Qingshan Chen, and Xiangyang Xu

quantitative real-time PCR. The leaves were collected from the VIGS-treated seedlings and the control seedlings at 25 d after infiltration. RNA extraction and cDNA synthesis were carried out as mentioned above. Quantitative real-time PCR (qRT-PCR) was performed