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Zhiyong Hu, Qing Liu, Meilian Tan, Hualin Yi, and Xiuxin Deng

), polyphenoloxidase (PPO), and peroxidase (POD) activities were assayed by the methods described by Sewalt et al. (1997) , Galeazzi et al. (1981) , and Hammerschmidt and Kuc (1982) , respectively. 4-coumarate: coenzyme A ligase (4CL) and cinnamyl alcohol

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Thanidchaya Puthmee, Kenji Takahashi, Midori Sugawara, Rieko Kawamata, Yoshie Motomura, Takashi Nishizawa, Toshiyuki Aikawa, and Wilawan Kumpoun

a correlation between lower POD activity and inferior periderm development. Fig. 8. Changes in ( A ) peroxidase activity and ( B ) ethylene production during fruit development. Different letters within columns indicate significant differences at P

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Hongmei Du, Zhaolong Wang, and Bingru Huang

the superoxide radical to H 2 O 2 ; peroxidase (POD); and catalase (CAT) that break down H 2 O 2 to water ( Smirnoff, 1993 ). Superior heat tolerance in warm-season species has been attributed to more efficient C 4 photosynthesis metabolism relative

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Ellen A. McComb, A. Raymond Miller, and Joseph C. Scheerens

Peroxidase activity in extracts from freeze-dried tissue of Fragaria × ananassa Duch. cv. Chandler was highest in tissue-cultured (TC) plants, followed by field-grown (FG) and lowest in greenhouse (GH) plants. Among tissue types, activity was highest in petioles, with leaves second highest. Fruit, root, and crown tissue all exhibited low or no activity. When subjected to isoelectric focusing (IEF), petiole tissue extracts exhibited more isozymes than extracts from other organs regardless of staining substrate. Using 4-chloro-1-naphthol and H2O2 as substrates, anionic and cationic isozymes were observed in TC petiole extract with nine isozyme bands ranging in pI from 3.9 to 9.5. In TC leaf extract an isozyme at pI 7.4 was observed that was not present in other organ extracts when H2O2 and benzidine, p-phenylenediamine or 3-amino-9-ethylcarbazole were used as substrates. Specific isozymes and number of isozymes varied according to plant organ and developmental stage. Mature leaves and over-ripe fruit appeared to exhibit more activity and a larger number of isozymes than developing tissues of those plant organs.

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M. Gómez Tena, M.A. Pedreño, A. Ros Barceló, and M.A. Ferrer

The subcellular localization of a basic peroxidase (EC 1.11.1.7) isoenzyme in crisphead lettuce (Lactuca sativa L.) leaves was studied through subcellular fractionation and protoplast and vacuole isolation. This isoenzyme is mainly located in soluble fractions. Studies using protoplast isolation and vacuole purification indicated that the soluble basic peroxidase isoenzyme is found in the vacuolar sap, probably in equilibrium with the same isoenzyme attached to tonoplast membranes.

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Lie-Bao Han, Gui-Long Song, and Xunzhong Zhang

. Plants have various antioxidant metabolites and enzymes to cope with the ROS. Peroxidase (POD) is an antioxidant enzyme that can scavenge H 2 O 2 . Research has shown that H 2 O 2 and POD are involved in cell wall stiffening during plant hypersensitive

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Guangxin Liu, Xiaoqian Su, Lingling Guan, and Fengrong Hu

biochemical metabolic pathways in plants, consequently affecting the growth and development of plants and pathogens ( Espinosa-Leal et al., 2018 ). Most reports have focused on enzymes such as phenylalanine ammonia-lyase, peroxidase, superoxide dismutase, and

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Yuji Yamada, Masayoshi Nakayama, Hiromitsu Shibata, Sanae Kishimoto, and Takashi Ikeda

Uvilla ( Aza-González et al., 2013 ). The enzymatic degradation of anthocyanin has also been detected in plants ( Barbagallo et al., 2007 ; Fang et al., 2015 ; Vaknin et al., 2005 ; Zipor et al., 2015 ), which may involve peroxidases, polyphenol

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Clíssia Barboza da Silva, Julio Marcos-Filho, Pablo Jourdan, and Mark A. Bennett

staining for peroxidase in bell pepper seeds of two cultivars, AF-6 and AF-7, following nondenaturing polyacrylamide gel electrophoresis. Each cultivar was represented by three and four seed lots, respectively. (a) Unprimed seeds; (b) drum-primed seeds

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Junqin Zong, Yanzhi Gao, Jingbo Chen, Hailin Guo, Yi Wang, Fan Meng, Yiwei Jiang, and Jianxiu Liu

, respectively ( Wang and Jiang, 2007 ). The decreased and unchanged SOD activities as well as differential responses of other antioxidant enzymes such as catalase (CAT), POD, and ascorbate peroxidase (APX) to waterlogging or submergence have also been found in