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Nina R.F. Castillo, Barbara M. Reed, Julie Graham, Felicidad Fernández-Fernández, and Nahla Victor Bassil

reliable means for cultivar identification and to assess genetic relatedness and diversity in these collections. Microsatellite markers were only recently developed from an expressed sequence tag library of ‘Merton Thornless’ ( Lewers et al., 2008 ). A

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Gayle M. Volk, Christopher M. Richards, Ann A. Reilley, Adam D. Henk, Patrick A. Reeves, Philip L. Forsline, and Herb S. Aldwinckle

represent 85 maternal sources of seeds (families) ( Table 1 ). All 496 individuals were analyzed using the seven microsatellite markers and a total of 126 alleles were amplified with an average gene diversity of 0.72 ( Table 2 ). Markers GD12, GD96, GD142

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Yuan Zhang, Chen Wang, HongZheng Ma, and SiLan Dai

-based aneuploid ( Zhang et al., 2013 ), indicating a complicated genetic background. Therefore, the read and data analysis is a major problem for the SSR analysis of chrysanthemums. Fortunately, a powerful method called microsatellite DNA allele counting

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Xinwang Wang, Deborah Dean, Phillip Wadl, Denita Hadziabdic, Brian Scheffler, Timothy Rinehart, Raul Cabrera, and Robert Trigiano

. Molecular tools are increasingly used for crape myrtle cultivar identification, parentage comparison, and interspecific hybrid analysis ( Pooler, 2003 ; Pounders et al., 2007 ). We report the development of microsatellites from crape myrtle ‘Natchez’ ( L

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Hafid Achtak, Ahmed Oukabli, Mohammed Ater, Sylvain Santoni, Finn Kjellberg, and Bouchaib Khadari

length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and microsatellites or simple sequence repeat (SSR)]. RAPD and AFLP markers are dominant and present limited reproducibility ( Jones et al

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Zhan Shu, Xue Zhang, Dianqiong Yu, Sijia Xue, and Hua Wang

( Hoban et al., 2009 ; Pollegioni et al., 2009 ; Ross-Davis et al., 2008 ; Wang et al., 2015 ). To investigate the possibility of genetic invasion, we used 12 microsatellite markers to identify the hybrids between J. regia and J . cathayensis in

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Hai-fei Yan, Xue-jun Ge, Chi-ming Hu, and Gang Hao

. obconica , suggesting that wild ones might be a potential resource for horticultural uses. Besides, attention to its adaptation to various habitats will facilitate discovering and cultivating more new horticultural characteristics. Nuclear microsatellite

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Li Lu, Kirk W. Pomper, Jeremiah D. Lowe, and Sheri B. Crabtree

. Microsatellite-enriched libraries and primer design. Genetic Identification Services (GIS, Chatsworth, CA) constructed pawpaw genomic libraries from DNA extracted from the cultivar PA-Golden (#1); libraries were enriched for di-nucleotide repeat GA (Library B

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Hussam S.M. Khierallah, Saleh M. Bader, Michael Baum, and Alladin Hamwieh

characterize a number of Iraqi date palm cultivars. Microsatellites, also known as SSR, are ideal DNA markers for population studies and genetic mapping as a result of their abundance, high level of polymorphism, dispersion throughout diverse genomes, ease to

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Chunxian Chen and William R. Okie

origins of plant cultivars and breeding lines ( Brundu et al., 2008 ; Gavrilenko et al., 2013 ; Molina-Cano et al., 2005 ; Riahi et al., 2011 ). Many microsatellite (also known as simple sequence repeat) markers have been developed from peach nuclear