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Axel O. Ramírez-Madera and Michael J. Havey

least five plants using the DNeasy ® Plant Maxi Kit protocol (Qiagen, Valencia, CA). We genotyped the candidate gene for zym in St 8, TMG-1, and the 49 RILs using the cleaved amplified polymorphic (dCAPS-G99A) marker developed by Amano et al. (2013

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Ke Cao, Lirong Wang, Gengrui Zhu, Weichao Fang, Chenwen Chen, and Pei Zhao

of 5 min at 72 °C. The PCR products were visualized by electrophoresis on 8% polyacrylamide gels run for 1 h at 250 W with 1 × TBE. Sequence-related amplified polymorphism analysis. For SRAP analysis, 14 forward primers (me1 to me14) and 17 reverse

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Karen R. Harris, Kai-Shu Ling, W. Patrick Wechter, and Amnon Levi

elite cultivar, normally required to select for those plants that would be homozygous for the recessive ZYMV resistance gene. In a recent study, we developed two cleaved amplified polymorphic sequence (CAPS) markers, CAPS-1 and CAPS-2, within the

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Guojing Li, Yonghua Liu, Jeffrey D. Ehlers, Zhujun Zhu, Xiaohua Wu, Baogeng Wang, and Zhongfu Lu

region (SCAR) or cleaved amplified polymorphism sequences (CAPS), is critical for both applications. The converted SCARs and CAPS are highly reliable, relatively inexpensive, and can be easily manipulated. Thus, they are valuable in practical breeding

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Dong Liu, Ping Li, Jiulong Hu, Kunyuan Li, Zhenyu Zhao, Weiyan Wang, Jinyuan Zhang, Xu Ding, and Zhimou Gao

. Melchinger, A.E. Lübberstedt, T.H. 2002 Genetic diversity in Arabidopsis thaliana L. Heynh. investigated by cleaved amplified polymorphic sequence (CAPS) and inter-simple sequence repeat (ISSR) markers Mol. Ecol. 11 495 505 Breton, C. Pinatel, C. Medail, F

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Chunxian Chen, Jude W. Grosser, Milica Ćalović, Patricia Serrano, Gemma Pasquali, Julie Gmitter, and Fred G. Gmitter Jr

as random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), cleaved amplified polymorphic sequence (CAPS), and simple sequence repeat (SSR) ( Deng et al., 1992 ; Fu et al., 2004 ; Grosser et al., 1995 , 2004 ; Xu et

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Alessandra Ghiani, Noemi Negrini, Silvia Morgutti, Federica Baldin, Fabio F. Nocito, Anna Spinardi, Ilaria Mignani, Daniele Bassi, and Maurizio Cocucci

-Blunt II-TOPO® vector (Invitrogen, San Giuliano, Italy), and sequenced (Primm). To discriminate length differences (InDel polymorphisms), a region (bp 1455-1892; Fig. S1 ) of the Pp-endo-PG gene comprising the major InDels was amplified (primers: FW

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Amnon Levi, Karen R. Harris-Shultz, and Kai-shu Ling

-assisted selection using two cleaved amplified polymorphic sequence (CAPS) markers in the eIF4E gene locus (CAPS1, CAPS2) as described by Ling et al. (2009) ( Fig. 2 ) and a sequenced characterized amplified region marker (designated as ZYRP) associated with ZYMV

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Derek W. Barchenger, Joseph I. Said, Yang Zhang, Mingzhou Song, Franchesca A. Ortega, Yeasong Ha, Byoung-Cheorl Kang, and Paul W. Bosland

.) Curr. Genet. 49 59 67 Kim, D.S. Kim, D.H. Yoo, J.H. Kim, B.D. 2006 Cleaved amplified polymorphic sequence and amplified fragment length polymorphism markers linked to the fertility restorer gene in chili pepper ( Capsicum annuum L.) Mol. Cells 21 135

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Aliya Momotaz, John W. Scott, and David J. Schuster

amplification. Genomic DNA was isolated from fully expanded leaf tissues of the F 2 plants (selected for lower disease severity and more vigor) using a simple DNA isolation procedure ( Fulton et al., 1995 ). Cleaved amplified polymorphic sequence (CAPS