provide a fast and accurate determination of nuclear DNA content that is related directly to ploidy level (among closely related taxa) and can be used as a taxonomic tool ( de Laat et al., 1987 ; Doležel, 1991 ; Doležel et al., 1998 ; Galbraith et al
Thomas G. Ranney, Nathan P. Lynch, Paul R. Fantz, and Paul Cappiello
Jason D. Lattier, Thomas G. Ranney, Paul R. Fantz, and Tony Avent
( Fantz, 1993 ). The complex taxonomy of liriopogons has been developing since the initial designation of Convallaria japonica by Thunberg (1780) . The following centuries resulted in many genera designations ( Anemarrhena Bunge, Chloopsis Blume
P. Obara-Okeyo, K. Fujii, and S. Kako
Eight enzyme systems were used to study electrophoretic variability among 12 species of Cymbidium Swartz and to assess phylogenetic relationships among them. The species could be easily distinguished by two enzyme systems, malate dehydrogenase (MDH) and phosphoglucose isomerase (GPI), although other enzyme combinations were also diagnostic. Genetic similarity index data indicated considerable genetic variability among the 12 species. Isozyme data supported the current taxonomic placement of the investigated species. The terrestrials [Cymbidium goeringii (Rchb. f.) Rchb. f., Cymbidium ensifolium (L.) Swartz, and Cymbidium sinense (Jackson) Wild.], which are all members of the subgenus Jensoa (Rafin.) Seth & Cribb., were the most closely related.
Fifty nine morphological characters and isozyme band patterns of glutamate oxaloacetate transminase, peroxidase, glucose phosphate isomerase from fully expanded leaves were used for taxonomic study on 51 taxa consisted of Korean native and principal cultivars of the genus Pyrus. Taxonomic relationships were analyzed by complete cluster analysis method based on Euclidean taxonomic distance of IBM PC SPSS/PC+(ver. 3.0). Among the 39 qualitative morphological characters, a great deal of variations among 51 taxa were observed in immature fruit shape, skin lusterness, hair density on pedicel, anther color, shape of leaf apex and base, hair density on leaf surface, and leaf margin. Considerable variations were found in most tested quantitative characters except in the number of petals and styles. More reliable taxonomic results could be obtained by comparing morphological characters rather than examining isozyme band patterns. Even though there were considerable differences depending upon the methods of investigation, classification of the genus Pyrus by using isozyme band patterns was proved to be a good tool for rapid taxonomic studies.
Svoboda V. Pennisi and Dennis B. McConnell
Detection of cuticular crystals in the 14 species of Dracaena examined indicated that they are probably ubiquitous throughout the genus and may permit rapid separation of dracaenas from plants with similar leaves such as the cordylines (Cordyline sp.). Dracaena species of the dragon tree group deposit the greatest quantity of uniformly small cuticular crystals. However, the distinction between individual species within this grouping, based solely on crystal numbers and size, is not sufficient for taxonomic separation. All other species of Dracaena studied did display species-specific quantities and sizes of cuticular crystals. This, in combination with characteristics of the leaf epidermis, could serve as part of a taxonomic key to the genus.
J. Kevin Parris, Thomas G. Ranney, Halina T. Knap, and W. Vance Baird
genome size using DAPI (AT preferential) or propidium iodide (PI) (intercalating) fluorochrome stains and estimate bp composition for representative taxa from 10 taxonomic sections. Materials and Methods Relative genome size and ploidy level determination
Richard A. Criley and George W. Staples
A new name for an old plant is not necessarily welcome in the horticultural trades or in plant identification classes, but some name changes have been in existence long enough that textbooks and trade publications should have caught up with them. The objective of this poster is to call attention to some of these changes for horticultural plant identification courses. Traditional references such as Hortus Third (1976) and Exotica 8 (Graf, 1976) have been superseded by the second edition of The Plant Book (Mabberly, 1997) and The Index of Garden Plants (Griffiths, 1994), while some recent works (The Tropical Look, Riffle, 1998) have chosen to retain old names. The taxonomic research underlying a new book, Tropical Garden Flora (Staples and Herbst, in press), based on the second edition of In Gardens of Hawaii (Neal, 1965), has produced an abundance of name changes. This poster will illustrate and report genera and species name changes that have occurred for selected ornamentals in the Acanthaceae, Agavaceae, Araceae, Araliaceae, Arecaceae, Commelinaceae, and Moraceae families plus a few others.
Robert E. Marra
Taxonomy, Phylogeny, and Ecology of Bark-inhabiting and Tree-pathogenic Fungi in the Cryphonectriaceae. M. Gryzenhout, B.D. Wingfield, and M.J. Wingfield. 2009. APS Press, St. Paul, MN 55121. 136 pages, 14 color images, 38 black and white images
M. Al-Zahim, H.J. Newbury, and B.V. Ford-Lloyd
RAPD analysis was employed to assess genetic variation in named cultivars of garlic (Allium sativum L.), and to examine the relationships between cultivated garlic varieties and the wild progenitor Allium longicuspis. Twenty-seven accessions were subjected to RAPD analysis using 26 oligonucleotide primers. Of a total of 292 bands, 63 (21%) were polymorphic. Cluster analysis revealed groupings that in part reflected patterns of morphological variation. All bolting forms (including wild and cultivated) grouped separately from the nonbolting cultivars. A. longicuspis and var. ophioscorodon grouped together, indicating close taxonomic affinity. Based upon relative levels of variation within different groups, we suggest potential relationships within the A. sativum/A. longicuspis complex.
William G. Hembree, Thomas G. Ranney, Nathan P. Lynch, and Brian E. Jackson
hybrids and cultivars has continued since then. The taxonomic history of Deutzia has seen it placed within both the Saxifragaceae and the Philadelphaceae families, although it is currently accepted as a member of Philadelpheae within Hydrangeaceae