Commercially processed citrus seeds of Carrizo citrange [Citrus sinensis (L.) Osb. × Poncirus trifoliata (L) Raf.], Swingle citrumelo (C. paradisi Macf. × P. trifoliata), Cleopatra mandarin (C. reticulate Blanco), and sour orange (C. aurantium L.) were used to test the effects of grading, hydrating, and priming on the rate of germination and seedling emergence. Sorting seed into groups by fresh weight or diameter did not generally improve seed performance. Seed fresh weight was highly correlated with maximum seed diameter; also, large seed weight and size were associated with a larger number of embryos. When seedlings from the extra embryos were removed, large seed produced the largest seedlings. Soaking seeds in aerated water significantly increased germination and emergence rates over unsoaked seeds. Soaking at 35C rather than 25C enhanced these differences. Priming seeds in one of three solutions of polyethylene glycol 6000 (—0.6 to—1.2 Mpa) was not successful> as germination and emergence Per centages were lower than in distilled water.
Eric H.C. Chilembwe, William S. Castle, and Daniel J. Cantliffe
G.B. McClure and N.S. Lang
Interconversions of seed storage reserves during osmoconditioning (controlled imbibition of water) may influence seed performance under suboptimal conditions. Sweet corn (Zen mays L. cv. Florida Staysweet) storage reserve changes were examined during osmoconditioning in relation to seed germination performance. Seeds were osmoconditioned in two experiments using distilled water (duration 3, 6, 9, 12, and 24 h) and polyethylene glycol 8000 solutions (0, .5, and 1.0 MPa for 12, 24, 48, 72 and 96 h). Germination performance was evaluated at 10 and 25C, and seed moisture, carbohydrate, and protein concentrations were quantified at each water potential x duration combination. Germination performance was not significantly improved by any treatment at 25C. Germination percentage at 10C was increased 10% for seeds osmoconditioned for 24 h in distilled water, and time to germination was decreased 50%. For seeds osmoconditioned 12 and 48 h at .5 and 1.0 MPa, respectively, germination percentage at 10C was increased 15%. Time to germination was reduced 50% for seeds osmoconditioned at .5 and 1.0 MPa after 48 and 96 h, respectively. Starch levels increased for seeds osmoconditioned at higher water potentials, but remained the same or decreased at lower water potentials.
Many warm-season turfgrass seeds have relatively poor germination percentages. Matriconditioning is a seed enhancement technique with a solid carrier and may be a practical solution to improve the germination characteristics of warm-season turfgrass. The objective of this study was to determine the effectiveness of matriconditioning on three nonaged and aged turfgrass cultivars: ‘Pensacola’ bahiagrass (Paspalum notatum), ‘Princess’ bermudagrass (Cynodon dactylon), and ‘Common’ centipedegrass (Eremochloa ophiuroides). Seeds were matriconditioned with a synthetic calcium silicate (MicroCel E) as a carrier and water at 30 °C for 5 days. Seed, carrier, and water ratio was 1 g, 0.5 g, and 1.5 mL, respectively. Matriconditioning increased final germination to 55% (bahiagrass), 90% (bermudagrass), and 70% (centipedegrass) compared with 92% in nontreated control seeds. Furthermore, matriconditioning decreased mean germination time 20% to 65% in all seeds compared with the nontreated control. Accelerated aging was induced by storing seeds for 0, 7, and 14 days at 42 °C and 95% relative humidity. Germination percentage decreased and mean germination time increased with the aging, especially after 14 days of aging treatment. These results suggest that matriconditioning is an effective technique to improve turfgrass seed performance.
Warley M. Nascimento
The growing of transplants in plug cell trays is the primary method of producing brassica transplants in many countries. Seed quality is an important aspect to achieve success in transplant production. Seed size may affect seed performance, seedling growth and development of brassica transplants. Seeds of cauliflower (`Vitoria de vero') and cabbage (`Unio') from Embrapa Vegetables were used in this study. During seed conditioning, seeds were classified using round screens generating three (>1.5, 1.5-2.0, and 2.0-2.5 mm) and four (>1.5, 1.5-2.0, 2.0-2.5, and < 2.5 mm) seed size categories, for cauliflower and cabbage, respectively. The original seed lot was used as control. Seed weight increased with seed size. Seed germination (laboratory) and seedling emergence (greenhouse) were not affected by seed size. In both species, root and shoot weight, and leaf area, measured 30 days after seeding, in greenhouse conditions, increased with seed size. Also, transplants from larger seed size resulted in a significantly higher root weight, shoot weight, and leaf area relative to the original (control) seeds. The results indicate that, overall, an adequate seed conditioning improve brassica transplant quality.
William J. Carpenter
Priming permits seeds to slowly imbibe water at regulated rates and to begin the initial stages of germination. Hypertonic polyethylene glycol (PEG) 8000 solutions of 1.0 and 1.2 MPa at 15C improved seed germination of dusty miller (Senecio cineraria DC.). At 0.8 MPa, germination was promoted during priming. No differences in rates, span, or total germination were found among seeds primed for 1, 2, or 3 weeks with or without aeration during priming. Germination percentages of primed and nonprimed seeds were similar at 10, 15, 20, and 25C, but 42% to 81% higher for primed seed at 30 or 35C. Priming reduced days to 50% of total germination (T50) 23% to 61%, and germination spans in days 30% to 67%. Primed seeds germinated most rapidly and uniformly at 20 and 25C. No change in total germination, T50, or germination span resulted when moisture contents of primed seeds were lowered to 7.8% or seeds were held at –80C for 7 days. Primed seed performance was unchanged after storage at 5C and 52% RH for 16 weeks.
J.E. Warren and M.A. Bennett
Osmopriming has been shown to enhance seed performance by increasing germination rates and uniformity. Furthermore, these enhancements persist under less-than-optimum conditions, such as salinity, reduced water availability, and excessively high or low temperatures. Additional benefits include resistance to soil pathogens due to lower leachate levels and more rapid emergence. To augment these existing qualities, it would be advantageous to incorporate beneficial organisms that antagonize soil-borne diseases, combining the benefits of both systems into a single procedure. To accomplish this, processing tomato seeds (Lycopersicon esculentum Mill. OH8245) were bioprimed in aerated –0.8 Mpa NaNO3 at 20°C for 4 days, at which time a mixture of nutrient broth, a defoaming agent, and beneficial bacteria that has been adjusted to the same osmotic potential is added. The bacteria used, Pseudomonas aureofaciens AB254, has been proved to control Pythium ultimum on a variety of crop seeds. After 7 days the seeds are removed having been primed and colonized with 105 colony forming units (cfu)/seed. In the absence of pathogen pressure, osmoprimed and bio-osmoprimed seeds performed similarly improving overall germination by 40% after 3 days, as well as low temperature (10–15°C) germination. However, when these seeds were sown in soilless media inoculated with P. ultimum, osmoprimed and bio-osmoprimed emergence was 57% and 74%, respectively, showing the improvements that these biologicals can provide. Thermogradient table results, storage tests, cfu/seed, and pathogen control will be discussed.
Alicia M. Borowski, Vincent A. Fritz, and Luther Waters Jr.
The objective of this study was to examine seed maturity at harvest as it relates to seed vigor in two commercial shrunken-2 (sh2 J sweet corn hybrids (Zea mays L. var rugosa Bonaf., cvs. Florida Staysweet, Crisp N' `Sweet 710). Seed harvest began at 0.76 g H2O/g fresh weight in 1987 and at 0.70 g H2O/g fresh weight in 1988 and 1989, and was continued at gradually declining moisture levels until frost. In five different tests of seed performance, seed of `Florida Staysweet' (FLASS) harvested between 0.23 to 0.57 g H2O/g fresh weight in 1987 possessed the highest seedling vigor. In 1988 and 1989, maximum vigor was achieved by FLASS seed harvested from 0.40 to 0.60 g H2O/g fresh weight and `Crisp N' Sweet 710' (CNS) seed harvested from 0.45 to 0.65 g H2O/g fresh weight. Standard germination test, seedling growth cold test (SGCT), and seed leachate conductivity provided the most consistent results to `determine optimum seed maturity. Seed weight was not as reliable an indicator of seed vigor in 1988 and 1989 as it was in 1987, and endosperm and embryo weights did not correlate with seedling vigor in any year.
Christopher Gunter, Senay Ozgen, Bjorn Karlsson, and Jiwan Palta
An increase in calcium concentration of potato tuber tissue has been shown to reduce soft rot severity and the incidence of internal physiological defects. Higher tuber calcium also seems to increase sprout vigor and maintain apical dominance by reducing subapical necrosis and sprout tip death. Preemergent applications of calcium at a rate of 0 and 26.5 kg·ha–1 from ammonium nitrate (PreAmNit), ammonium nitrate plus calcium nitrate (PreCaN), or calcium chloride plus calcium nitrate plus urea (PreCUC). A group of post-emergent split calcium nitrate plus calcium chloride plus urea (PostCUC) applications beginning with hilling and proceeding at 3, 6, and 8 weeks after hilling were also made at a rate of 56 kg·ha–1 calcium at each application time. From visual ratings of stand quality taken 64 days after hilling, we found plants receiving a preemergent application of nutrients or PostCUC had higher stand ratings than paired control plots. Internal tuber quality ratings revealed less internal brown spot in the PostCUC application in 168–364-g tubers. Yield of 112–168-g tubers was greatest from plants treated with PreCaN or PreCUC followed by PostCUC. PreAmNit plots had higher culls than the PreCUC plots. The non-split ammonium nitrate control (all nitrogen by hilling) produced a higher number of B-sized tubers than the PostCUC treatment. Also the PreAmNit+PostCUC had more B-sized tubers than PreCaN+PostCUC. In general the PostCUC treatment produced fewer small tubers and more large tubers than other treatments. These results suggest application of a small amount of calcium prior to emergence but after the sprouts have begun to develop improves seed performance. Furthermore these data show that supplemental calcium application during the season may improve tuber grade.
The effectiveness of directional phenotypic selection to improve tomato (Lycopersicon esculentum Mill.) seed germination under salt-stress was investigated. Seed of F2 and F3 progeny of F1 hybrids between a salt-tolerant (PI174263) and a salt-sensitive (UCT5) tomato cultivar were evaluated for germination response at three stress levels of 100 (low), 150 (intermediate), and 200 mm (high) synthetic sea salt (SSS). At each salt-stress level, the most tolerant individuals, as determined by the germination speed, were selected. Selected individuals (F2s or F3s) were grown to maturity and self-pollinated to produce F3 and F4 progeny families. The selected progeny from each experiment were evaluated for germination at four treatment levels of 0 (nonstress), 100, 150, and 200 mm SSS and were compared with unselected populations. The results indicated that selections were equally effective at all three salt-stress levels and in F2 and F3 generations and significantly improved seed germination of progeny under salt-stress and nonstress treatments. Estimates of realized heritability for rapid germination under the various salt-stress levels ranged from 0.67 to 0.76. Analysis of response and correlated response to selection indicated a genetic correspondence of up to 100% between germination at different salt-stress levels. Genotypic family correlations between germination at the low, intermediate, and high salt-stress levels ranged from 0.67 to 0.89, and those between nonstress and salt-stress conditions ranged from 0.25 (between 0 and 200 mm) to 0.71 (between 0 and 100 mm salt). The results indicated that similar or identical genes with additive genetic effects contributed to rapid germination response of tomato seeds at different salt-stress levels. Thus, selection at one stress level resulted in progeny with improved germination at diverse salt-stress levels. The results also indicated that to improve tomato seed germination, selection can be based on individual seed performance and early segregating generations.
needs. Matriconditioning Is a Cost-effective Seed Enhancement Technique for Warm-season Turfgrasses Poor seed germination is a fundamental limitation in establishing successful lawns. One approach for improving germination and seed performance in the