. Measurements. Predawn leaf water potential measurements were made twice a week in 2007 and once a week in 2008 using a pressure chamber (Model 3005HGPL; Soil, Moisture Equipment Corp, Santa Barbara, CA). At predawn, five stems of each species in each subplot
Nisa Leksungnoen, Paul G. Johnson, and Roger K. Kjelgren
Terence L. Robinson and Bruce H. Barritt
Abbreviations: ABA, abscisic acid; c, t-ABA, cis, trans-abscisic acid; LPI, leaf plastochron index; PI, plastochron index age; ψ P , leaf turgor pressure; ψ S , leaf osmotic potential; ψ W , leaf water potential; t , t-ABA, trans, trans
Jianping P. Wang, Suleiman S. Bughrara, and C. Jerry Nelson
( Festuca mairei ) compared with three tall fescue ( F. arundinacea Schreb.) cultivars: Kentucky 31, Falcon II, and Barolex. Objectives of this study were to compare leaf elongation, leaf water content, leaf water potential, root biomass, and root length
Chiara Cirillo, Youssef Rouphael, Rosanna Caputo, Giampaolo Raimondi, and Stefania De Pascale
, Cambridge, U.K.). The water potential components of leaves were measured psychrometrically on the same date of the stomatal resistance measurements using a dew-point psychrometer (WP4; Decagon Devices, Pullman, WA). Leaf water potential (Ψ w ) was measured
Sanjit K. Deb, Manoj K. Shukla, and John G. Mexal
. Measurement of stem water potential and leaf water potential. Midday ψ stem and ψ leaf (between 1400 and 1500 hr Mountain Standard Time) were determined simultaneously using a pressure chamber (Model 1000 with digital gauge; PMS Instrument Company, Albany
Y.L. Qian and J.D. Fry
`Meyer' zoysiagrass (Zoysia japonica Steud.) was established on a silt loam soil in 27-cm-diameter × 92-cm-deep containers in a greenhouse to investigate the influence of irrigation frequency on turfgrass rooting and drought tolerance. Turf was irrigated daily or at the onset of leaf rolling with a water volume equal to the cumulative evapotranspiration of well-watered turf in small weighing lysimeters. After >90 days of irrigation treatments, a dry-down was imposed during which no additional water was applied for 55 days. A recovery period followed during which time turf was watered to maintain soil matric potential at greater than –30 kPa. Compared to turf irrigated daily, that watered at the onset of leaf rolling exhibited 1) 32% to 36% lower leaf water potential and 14% to 22% lower osmotic potential before the onset of drought; 2) 13% higher leaf water potential ≈40 days into dry-down; 3) more extensive rooting at 55- and 75-cm soil depths as indicated by 11% to 19% lower volumetric soil moisture content at the end of dry-down; 4) 25% to 40% lower shoot growth rate during irrigation and 13% to 33% higher shoot growth rate during dry-down; and 5) higher quality ratings during dry-down and recovery. Thus, deep, infrequent irrigation better prepares zoysiagrass for an oncoming drought than light, frequent irrigation.
R. Savé M. Pery, O. Marfà, and L. Serrano
Two experiments were conducted to assess the ability of a water-absorbing synthetic polymer to reduce water stress injury of seedlings of Pinus pinea L. under greenhouse and field conditions. In both experiments, two rates of hydrated hydrogel, corresponding to 200 and 400 cm3 of stored water, and a control treatment without hydrophilic polymer were tested. Survival periods for the pine seedlings were 1.4 and 2.0 times longer for the 200- and 400-cm3 treatments, respectively, than for a control treatment in a greenhouse assay. In the field assay, only differences in seedling survival between both hydrogel treatments and control were measured. Leaf water potential values of control plants were significantly lower than hydrated polymer treatments in both experiments. From these results, we conclude that the use of hydrophilic polymers may be an important method of increasing the success of reforestation in semiarid regions.
S.M. Lutfor Rahman, Wayne A. Mackay, Eiji Nawata, Tetsuo Sakuratani, A.S.M. Mesbah Uddin, and Bruno Quebedeaux
Effects of water stress on superoxide dismutase (SOD) activities, changes in protein content, leaf water potential (Ψl) and growth were studied in drought-sensitive Kyokko (KK) and Ratan (RT), and drought-tolerant TM 0126 (TM) and VF-134-1-2 (VF) cultivars of tomato (Lycopersicon esculentum Mill.) in order to obtain fundamental information for breeding drought tolerant cultivars that may be adapted to water stress in many parts of the world. Growth of drought-tolerant TM and VF was greater than that of drought-sensitive KK and RT under water stress conditions. Leaf water potential (Ψl) decreased by water stress treatments in all the cultivars, but the reduction was much more rapid and pronounced in KK and RT than VF and TM. Ψl of stressed cultivars decreased by 30% to 40% compared to the untreated control cultivars. The initial reduction in the range of 20% to 35% was more rapid in KK and RT than TM and VF. SOD activities were increased by water stress in all cultivars. Increase of SOD activities by water stress was much more rapid and pronounced in TM and VF than in KK and RT. Leaf protein concentration was decreased by the water stress treatments in all cultivars evaluated. In KK and RT, much more rapid reductions in protein concentration were observed than in TM and VF. The regression analysis of Ψl and SOD suggest the possibility to using SOD activities as an additional screening criterion for tomato drought tolerance improvement.
R.A. Stern, M. Meron, A. Naor, R. Wallach, B. Bravdo, and S. Gazit
The effect of fall irrigation level in `Mauritius' and `Floridian' lychee (Litchi chinensis Sonn.) on soil and plant water status, flowering intensity, and yield the following year was studied in a field during 2 consecutive years. At the end of the second vegetative flush after harvest (1 Oct. 1994 and 10 Oct. 1995), four irrigation treatments were initiated: 0.5, 0.25, 0.125, and 0 Class A pan evaporation coefficients designated 100%, 50%, 25%, and 0%. The three lower irrigation levels effectively stopped shoot growth, suggesting the 50% treatment to be the threshold for shoot growth cessation in both years. For both years, flowering intensity and yield in the 100% treatment were lower than those following the other three treatments. Soil and plant water-stress indicators responded to the water-stress irrigation treatments. However soil water-potential values were highly variable relative to plant water potentials. Stem water potential differed more markedly between treatments than leaf water potential. Midday stem water potential appeared to be the best water-stress indicator for irrigation control. Midday stem water potential in both years was correlated with midday vapor-pressure deficit, suggesting that the threshold for irrigation control should take into account evaporative demand.
Jun Ying Zhao, Li Jun Wang, Pei Ge Fan, Zhan Wu Dai, and Shao Hua Li
Half or whole root systems of micropropagated `Gala' apple (Malus ×domestica Borkh.) plants were subjected to drought stress by regulating the osmotic potential of the nutrient solution using polyethylene glycol (20% w/v) to investigate the effect of root drying on NO3- content and metabolism in roots and leaves and on leaf photosynthesis. No significant difference in predawn leaf water potential was found between half root stress (HRS) and control (CK), while predawn leaf water potential from both was significantly higher than for the whole root stress (WRS) treatment. However, diurnal leaf water potential of HRS was lower than CK and higher than WRS during most of the daytime. Neither HRS nor WRS influenced foliar NO3- concentration, but both significantly reduced NO3- concentration in drought-stressed roots as early as 4 hours after stress treatment started. This reduced NO3- concentration was maintained in HRS and WRS roots to the end of the experiment. However, there were no significant differences in NO3- concerntation between CK roots and unstressed roots of HRS. Similar to the effect on root NO3- concentration, both HRS and WRS reduced nitrate reductase activity in drought-stressed roots. Moreover, leaf net photosynthesis, stomatal conductance and transpiration rate of HRS plants were reduced significantly throughout the experiment when compared with CK plants, but the values were higher than those of WRS plants in the first 7 days of stress treatment though not at later times. Net photosynthesis, stomatal conductance and transpiration rate were correlated to root NO3- concentration. This correlation may simply reflect the fact that water stress affected both NO3- concentration in roots and leaf gas exchange in the same direction.