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Winthrop B. Phippen and James E. Simon

A plant regeneration protocol was successfully developed for basil (O. basilicum L.). Explants from 1-month-old seedlings yielded the highest frequency of regeneration of shoots (37%) with an average number of 3.6 shoots per explant. Calli and shoot induction were initiated on Murashige and Skoog (MS) basal medium supplemented with thidiazuron (TDZ) (4 mg/L) for ≈30 days. Shoot induction and development was achieved by refreshing the induction medium once after 14 days. The most morphogenetically responsive explants were basal leaf explants from the first fully expanded true leafs of greenhouse-grown basil seedlings. Developing shoots were then rooted on MS media in the dark without TDZ. Within 20 days, rooted plantlets were transferred and acclimatized under greenhouse conditions where they developed normal morphological characteristics. This is the first report of a successful in vitro regeneration system for basil through primary callus. The establishment of a reliable regeneration procedure is critical when developing a transformation protocol for enhancing the production of basil for insect and disease resistance and improved essential oil constituents.

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Brian A. Kahn, Niels O. Maness, Donna R. Chrz, and Lynda K. Carrier

grown basil ( Ocimum basilicum L.) Tidsskr. Planteavl 96 499 506 Takano, T. 1993 Germination characteristics of herbs in Labiatae Acta Hort. 331 275 286

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Kellie J. Walters and Christopher J. Currey

development (SPAD) meter index, and nutrient concentrations for sweet basil ( Ocimum basilicum ‘Nufar’), lemon basil ( Ocimum × citriodorum ‘Lime’), and holy basil ( Ocimum tenuiflorum ‘Holy’) 3 weeks after transplanting into nutrient-film technique

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Kathryn Homa, William P. Barney, Daniel L. Ward, Christian A. Wyenandt, and James E. Simon

stomata may be associated with resistance or susceptibility. Fig. 1. ( A ) Stomata (round) cast image from abaxial surface of Ocimum basilicum (‘Nufar’). ( B ) Stomata (oval) cast image from abaxial surface of Ocimum × africanum (‘Lemon’). ( C

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Fernanda Trientini and Paul R. Fisher

. Values express mg of nutrient obtained from 1 g of milled fertilizer measured by laboratory analysis. Values represent an average of three samples. Expt. B: Basil plant growth with two commercial CRF products. Growth of hydroponic basil ( Ocimum basilicum

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Samuel Doty, Ryan W. Dickson, and Michael Evans

ornamental crops tend to differ in system design and nutrient and water delivery. Fresh market basil ( Ocimum basilicum ), for example, is typically produced in hydroponic systems in which plant roots are submerged in nutrient solution to promote maximum

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T.J. Radovich, C.G. Cavaletto, and H.R. Valenzuela

The sensory quality of fresh basil (cv. Sweet Italian) was evaluated to determine if compost or mineral fertilizer applications affected flavor and aroma intensity. The four treatments, arranged in a randomized complete-block design with three replications, were: compost applied at 50 t·ha–1; compost applied at 200 t·ha–1; mineral N applied at 110 kg·ha–1; and a control receiving no amendment. Leaves from the first four nodes of young shoots were used in the evaluation. Twelve trained panel members scored samples of three leaves from each treatment for aroma and flavor intensity using a linear scale, converted to a scale of 1–10 where 1 = much less intense than a reference sample (control), and 10 = much more intense than the reference. No significant difference between treatments was found in flavor intensity. Significant difference between treatments in aroma intensity was found. Aroma scores were highest in samples from the compost and synthetic fertilizer treatments, and lowest in those from the control. Scores for aroma from the compost and synthetic fertilizer treatments were similar to each other. Aroma intensity increased with increased rate of compost application. No significant correlation was found between aroma intensity and plant tissue N content, sap NO3 levels, or yield.

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Menahem Edelstein, Daniel Berstein, Moshe Shenker, Hasan Azaizeh, and Meni Ben-Hur

.B. 2003 Pattern of selenium distribution in tomato Lycopersicum esculentum Mill. Biol. Bull. 30 468 471 Hawrylak-Nowak, B. 2008 Enhanced selenium content in sweet basil ( Ocimum basilicum L.) by foliar fertilization Veg. Crops Res. Bull. 69 63 72 Hurd

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Elisa Solis-Toapanta, Paul Fisher, and Celina Gómez

. Basil ( Ocimum basilicum ) plants are suitable for indoor gardening as they can successfully be grown in a variety of conditions such as temperature ranges from 10 to 27 °C, EC from 0.5 to 4.0 dS·m −1 , pH from 4.3 to 8.2, and daily light integrals (DLIs

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Robert M. Pyne, Adolfina R. Koroch, Christian A. Wyenandt, and James E. Simon

oleracea Hereditas 141 293 300 Cohen, Y. Vaknin, M. Ben-naim, Y. Rubin, A.E. Silverman, D. Bitton, S. Adler, U. 2013 First report of the occurrence and resistance to mefenoxam of Peronospora belbahrii, causal agent of downy mildew of basil (Ocimum basilicum