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Timothy F. Wenslaff and Paul M. Lyrene

A study was done to test whether inheritance is tetrasomic or disomic in tetraploid Fl hybrids between 2x Vaccinium elliottii Chapm. and 4x V. corymbosum L. Seventeen Fl hybrids derived from V. elliottii homozygous for recessive anthocyanin deficiency (AD) were confirmed by isozyme analysis and, where V. elliottii was the seed parent, by the presence of anthocyanin. Fertile hybrids with high pollen stainability were assumed to be 4x and duplex for the AD allele, having arisen from 2n gametes in V. elliottii. In nine Fl × Fl crosses, all progeny populations segregated for AD phenotype at or above the expected tetrasomic ratio of 1 AD:35 normal; no AD would be expected with disomic inheritance. Tetraploid AD progeny were used in testcrosses on sixteen Fl hybrids in 1991. Progeny segregated tetrasomically, 1 AD:5 normal. Isozyme loci PGD-2 and PGI-2 also segregated tetrasomically.

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Luping Qu and James F. Hancock

A tetraploid blueberry population resulting from a cross of US 75 {a tetraploid hybrid of Fla 4B [a selection of Vaccinium darrowi Camp (2n = 2x = 24) × `Bluecrop' [(V. corymbosum L. (2n = 4x = 48)]} × `Bluetta' (4x) was used to generate a genetic linkage map of US 75 by randomly amplified polymorphic DNA (RAPD) analysis. One hundred and forty markers unique for Fla 4B that segregated 1:1 in the population were mapped into 29 linkage groups that cover a total genetic distance of 1288.2 cM, with a range of 1.6 to 33.9 cM between adjacent markers. The map is essentially of V. darrowi because US 75 was produced via a 2n gamete from Fla 4B and only unique markers for Fla 4B were used. Therefore, all the chromosomes of V. darrowi could be represented in the map.

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Kathleen G. Haynes

Although potato (Solanum tuberosum L.) tuber yellow flesh per se is known to be controlled by a single gene, the intensity of yellow flesh varies widely in Solanum L. species. Many diploid species have very intense yellow flesh, as compared to the commercial tetraploid yellow-flesh cultivar `Yukon Gold'. Inheritance of yellow-flesh intensity at the diploid level was investigated in a hybrid population of S. phureja ssp. phureja (Juz. & Buk.)-S. stenotomum ssp. stenotomum (Juz. & Buk.) (PHU-STN). Six randomly chosen male parents were crossed to five randomly chosen female parents in a Design II mating scheme. In 1993, ≈12 progeny (clones) from each of the 30 families were planted in a randomized complete block design with two replications in Presque Isle, Maine, and evaluated for tuber yellow-flesh intensity as measured by a reflectance colorimeter. Twenty-five tubers from each plot were scored using the YI E-313 yellow intensity scale. An average YI E-313 score was obtained for each plot. Narrow-sense heritability on a plot mean basis was estimated as 0.99 with a SE of 0.65 to 0.72. There were significant differences among clones within a family. Results suggest that rapid progress can be made in breeding for intense yellow flesh in this diploid population. Clones from this population that produce 2n gametes represent an important source of germplasm for enhancing the intensity of the yellow-flesh trait in tetraploid potatoes.

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Pablo Aleza, José Cuenca, José Juárez, José A. Pina, and Luis Navarro

et al., 2002 ; Ollitrault et al., 2008 ). One exploits natural events of polyploidization such as 2n gametes using embryo rescue and flow cytometry to select triploids in 2x × 2x crosses. Second meiotic division restitution has been proposed for

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Shujun Zhou, Guixue Zhou, and Kehu Li

., 2006 ). Triploid lilies (2 n = 3 x = 36) are also produced by diploid × tetraploid crosses or 2n gametes ( Barba-Gonzalez et al., 2004 , 2005a , 2005b ; Lim et al., 2000 ). Based on their genome composition, triploid lilies with three identical

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Shujun Zhou, Xin Tan, Liqin Fang, Jia Jian, Ping Xu, and Guoliang Yuan

of 2 n gametes for the production of sexual polyploids from sterile Oriental × Asiatic hybrids of lilies ( Lilium ) Theor. Appl. Genet. 109 1125 1132 Barba-Gonzalez, R. van Silfhout, R.A.A. Ramanna, M.S. Visser, R.G.F. van Tuyl, J.M. 2006 Progenies

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Jonathan D. Mahoney, Thao M. Hau, Bryan A. Connolly, and Mark H. Brand

produced two tetraploid offspring that could have been either self-fertilized with an unreduced 2 n gamete or out-crossed with a reduced 2 n gamete from a tetraploid Aronia spp. Similarly, UC141, UC145, UC146, UC150, and UC156 ( A. prunifolia , 3

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Yifei Wang, Stephanie K. Fong, Ajay P. Singh, Nicholi Vorsa, and Jennifer Johnson-Cicalese

tetraploid and hexaploid is facilitated by widespread 2n gamete production in diploid blueberry species ( Ortiz et al., 1991 , 1992 ; Qu and Vorsa, 1999 ). In the current study, we selected three commercial tetraploid (4 x ) northern highbush blueberry ( V

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Lisa Alexander

autopolyploids are likely the result of undetected 2 n gamete formation ( Bretangolle and Thompson, 1995 ), molecular marker analysis is necessary to verify the origin of these unexpected polyploids. Influence of ploidy on floral characteristics. A total of 86

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Mark K. Ehlenfeldt, Lisa J. Rowland, Elizabeth L. Ogden, and Bryan T. Vinyard

blueberry: Germplasm transfer and 2n gametes in blueberry Acta Hort. 346 95 102 Flinn, C.L. Ashworth, E.N. 1994 Blueberry flower-bud hardiness is not estimated by differential thermal analysis J. Amer. Soc. Hort. Sci