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Enrique I. Sánchez-González, Adriana Gutiérrez-Díez, and Netzahualcóyotl Mayek-Pérez

. Table 1. Simple sequence repeat description and genetic variation parameters per locus of Mexican race avocados. ISSR markers PCR was carried out in a 25-µL volume containing molecular biology-grade water, 1× PCR buffer (Promega), 3 m m MgCl 2 , 0.25 m

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Josh A. Honig, Stacy A. Bonos, and William A. Meyer

Heckenberger, M. Bohn, M. Melchinger, A.E. 2005a Identification of essentially derived varieties obtained from biparental crosses of homozygous lines: I. Simple sequence repeat data from maize inbreds Crop Sci. 45 1120 1131 Heckenberger, M. Bohn, M. Klein, D

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Masanori Honjo, Susumu Yui, and Miyuki Kunihisa

phase. However, Rousseau-Gueutin et al. (2008) suggested that disomic behavior is predominant in the cultivated strawberry on the basis of comprehensive mapping using AFLP and simple sequence repeat (SSR) markers. Similarly, more recent linkage

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Hatsuhiko Okada, Yoshitaka Ohashi, Mamoru Sato, Hideyuki Matsuno, Toshiya Yamamoto, Hoytaek Kim, Tatsuro Tukuni, and Sadao Komori

) following the manufacturer's instructions with slight modifications ( Yamamoto et al., 2006 ). Confirmation of anther culture-derived plantlets assessed by simple sequence repeat (SSR) markers. Thirty-five SSR markers were used for investigating the

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Dario J. Chavez, Thomas G. Beckman, and José X. Chaparro

, 2006 ). Uncorrected p distances from aligned sequences were calculated in PAUP*, version 4.0b10 and were used as input in POFAD. Results Simple sequence repeats The 41 SSR markers amplified 471 alleles in the 11 species representing outgroups and the

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Jude W. Grosser, Hyun Joo An, Milica Calovic, Dong H. Lee, Chunxian Chen, Monica Vasconcellos, and Frederick G. Gmitter Jr

sequence repeat primers and genotyping procedure for somatic hybrid and autotetraploid verification. The eight expressed sequence tag–simple sequence repeat (EST-SSR) primers, CX6F04, CX6F18, CX6F29, CX5F57, CX0010, CX0035, CX2007, and CX2021, used in

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Cecilia McGregor, Vickie Waters, Savithri Nambeesan, Dan MacLean, Byron L. Candole, and Patrick Conner

initial stepdown was carried out for six cycles followed by 33 cycles at the base annealing temperature. The amplification was followed by a final extension step of 15 min at 72 °C. Table 1. Simple sequence repeat primers used in this study. Amplifications

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Xiu Cai Fan, Hai Sheng Sun, Ying Zhang, Jian Fu Jiang, Min Li, and Chong Huai Liu

( He ) heterozygosity, and polymorphic information content (PIC) of the 11 simple sequence repeat loci analyzed. In this study, the percentage of heterozygotes of the 11 loci was high, indicating the high variability present in the samples. The He

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Li Lu, Kirk W. Pomper, Jeremiah D. Lowe, and Sheri B. Crabtree

were labeled with FAM or HEX and were made by Integrated DNA Technologies (Coralville, IA). Simple sequence repeat–polymerase chain reaction amplification. The SSR–polymerase chain reaction (PCR) amplification was performed with GoTaq Flexi DNA

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Fuad Gasi, Silvio Simon, Naris Pojskic, Mirsad Kurtovic, Ivan Pejic, Mekjell Meland, and Clive Kaiser

included in plant breeding programs established in the region. Table 1. Seventy one apple accessions (31 sampled from farms in Sarajevo and 40 from farms in eastern Bosnia) analyzed in this study using 10 simple sequence repeat markers. z Table 2. Twenty