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Kahraman Gürcan and Shawn A. Mehlenbacher

variation in chromosome number and genome size among species of the Betulaceae, some degree of microsatellite marker transferability is expected based on results in other plant families. Microsatellites, also known as simple sequence repeats (SSR), are

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Edward J. Boza, Juan Carlos Motamayor, Freddy M. Amores, Sergio Cedeño-Amador, Cecile L. Tondo, Donald S. Livingstone III, Raymond J. Schnell, and Osman A. Gutiérrez

markers such as simple sequence repeat (SSR) and single nucleotide polymorphism are used to study genetic identity and evolutionary relationships in germplasm collections. In cacao, these markers have been used for establishing genotype identities, genetic

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Li Li, Ling Liu, Deshuang Zhang, Ping Wu, Fenglan Zhang, and Xiulan Xu

growing tendency. Therefore, purity percentages determined by SSR methods were higher than those determined by the GOT by no more than 5.0%. Table 5. Hybrid purity percentage as determined by simple sequence repeat (SSR) markers and by the grow-out test

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Liwang Liu, Guang Liu, Yiqin Gong, Wenhao Dai, Yan Wang, Fanmin Yu, and Yunying Ren

, R.V. 2002 Assessment of purity of rice hybrids using microsatellite and STS markers Crop Sci. 42 1369 1373 Zietkiewicz, E. Rafalski, A. Labuda, D. 1994 Genome fingerprinting by simple sequence

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Marcin Nowicki, Edward E. Schilling, Sarah L. Boggess, Logan C. Houston, Matthew L. Huff, Margaret E. Staton, Jayne A. Lampley, and Robert N. Trigiano

confirmed to advance by 31 d over 30 years of studies, ranking it as the fifth most altered among the 89 plant species investigated ( Abu-Asab et al., 2001 ). Among the neutral molecular markers, the microsatellites or simple sequence repeats (SSRs) are

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Colton Ives, Vidyasagar R. Sathuvalli, Brooke C. Colburn, and Shawn A. Mehlenbacher

a Nanodrop spectrophotometer (NanoDrop, Wilmington, DE) and diluted with Tris-EDTA buffer to a concentration of 20 ng·μL −1 . Fig. 1. Pedigrees of two hazelnut progenies segregating for S-alleles, color, and simple sequence repeat markers

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Barbara Gilmore, Nahla Bassil, April Nyberg, Brian Knaus, Don Smith, Danny L. Barney, and Kim Hummer

protocol for the library preparation was performed using Qiagen (Valencia, CA) reagents ( Gilmore et al., 2011 ). Table 1. Peony ( Paeonia ) species used for DNA sequence determination and for designing simple sequence repeat (SSR) primers. z DNA sequencing

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Caihong Wang, Yike Tian, Emily J. Buck, Susan E. Gardiner, Hongyi Dai, and Yanli Jia

final extension step at 72 °C for 5 min. The products were visualized on a 1.3% agarose gel as previously described. Simple sequence repeat marker screening. A total of 51 SSR primers ( Table 1 ) was used in the BSA approach to identify SSR markers

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Min Zhang, Xiuxin Deng, Changping Qin, Chunli Chen, Hongyan Zhang, Qing Liu, Zhiyong Hu, Linlin Guo, Wenhua Song, Yong Tan, and Shengcai Liao

markers, such as simple sequence repeats (SSRs), biochemical components, and morphology etc. In fact, morphological ( Marcotrigiano and Bernatzky, 1995 ; Szymkowiak and Irish, 1999 ; Szymkowiak and Sussex, 1992 ), cytological ( Chen et al., 2006

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Enrique I. Sánchez-González, Adriana Gutiérrez-Díez, and Netzahualcóyotl Mayek-Pérez

. Table 1. Simple sequence repeat description and genetic variation parameters per locus of Mexican race avocados. ISSR markers PCR was carried out in a 25-µL volume containing molecular biology-grade water, 1× PCR buffer (Promega), 3 m m MgCl 2 , 0.25 m