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Mathews L. Paret, Ryo Kubota, Daniel M. Jenkins, and Anne M. Alvarez

. None of the samples in the control pots with no Rs inoculum tested positive. The detection capability of PCR- fliC was above 80% when populations of Rs race 4 in drainage water from soil were above 3 log cfu/mL. An even more sensitive real-time PCR

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Mundaya N. Jithesh, Owen S.D. Wally, Iain Manfield, Alan T. Critchley, David Hiltz, and Balakrishnan Prithiviraj

transcript levels were assayed by real-time polymerase chain reaction (PCR) analysis using gene-specific primers on a StepOne™ Real-Time PCR System (Applied Biosystems) using SYBR gene reagent power SYBR (Applied Biosystems). Primer sequences were taken from

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Zehuang Zhang, Qihua Lin, and Qiuzhen Zhong

the two samples. Quantitative real-time reverse transcription PCR analysis. The first-strand cDNA was synthesized from 1.0 μg DNA-free RNA using the AMV first-strand cDNA Synthesis Kit (SK2445) according to the manufacturer’s protocol. The quantitative

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Monrudee Kittikorn, Katsuya Okawa, Hitoshi Ohara, Satoru Kondo, Nobuhiro Kotoda, Masato Wada, Mineyuki Yokoyama, Ohji Ifuku, Ariake Murata, and Naoharu Watanabe

. The product was identified with HPLC-MS/MS. The fragment ions of CKODA were m/z 363 [M-H] – and 345 [M-H-H 2 O] – . RNA extraction, c DNA synthesis, and quantitative real-time RT-PCR. Total RNA from apical buds was extracted following the protocol

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Shutian Tao, Danyang Wang, Cong Jin, Wei Sun, Xing Liu, Shaoling Zhang, Fuyong Gao, and Shahrokh Khanizadeh

ReverTra Ace qPCR RT Kit (Toyobo, Osaka, Japan) following the manufacturer’s recommendations to a final volume of 20 μL. Quantitative real-time RT-PCR. The primers used in the present study to investigate the C4H expression pattern in fruit tissues with

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Lili Zhuang, Mengxian Liu, Xiuyun Yuan, Zhimin Yang, and Bingru Huang

for Biotechnology Information [NCBI ( Gish and States, 1993 )]. Characterization of FaPIP2;1 expression in response to drought stress. FaPIP2;1 expression in tall fescue responding to drought stress was examined using quantitative real-time reverse

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Heidi Hargarten, Sumyya Waliullah, Lee Kalcsits, and Loren A. Honaas

Real-Time PCR Detection System (catalog no. 1855195; Bio-Rad Laboratories) using SsoAdvanced Universal SYBR ® Green Supermix (catalog no. 1725270; Bio-Rad Laboratories). The reaction volume was 10 µL, the template mass per reaction was 10 pg cDNA, and

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Tripti Vashisth, D. Scott NeSmith, and Anish Malladi

and stored at −20 °C until further analysis. Gene expression analysis was performed using quantitative real-time polymerase chain reaction (qRT-PCR). Twenty-eight genes related to cell wall and membrane metabolism, phytohormone metabolism and signaling

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David Gopaulchan, Adrian M. Lennon, and Pathmanathan Umaharan

, ANS , DFR , and F3′H genes were examined at different stages of spathe development using reverse transcription quantitative real-time PCR (RT-qPCR) in five pink-spathed cultivars, with different color shade intensities and representing known

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Nobuhito Mitani, Atsushi Kono, Masahiko Yamada, Akihiko Sato, Shozo Kobayashi, Yusuke Ban, Toshihito Ueno, Mikio Shiraishi, Shinya Kanzaki, Tomoyuki Tsujimoto, and Keizo Yonemori

, M. Tao, R. Parfitt, D.E. Yonemori, K. 2009 Quantitative real-time PCR to determine allele number for the astringency locus by analysis of a linked marker in Diospyros kaki Thunb Tree Genet. Genomes 5 483 492 Akagi, T. Takeda, Y. Yonemori, K